Results 81 to 90 of about 67,710 (307)
Functional validation of cancer stem cell markers in primary human colorectal cancer and established cell lines [PDF]
, 2014 In an increasing number of cancers, CSCs have been defined on the basis of the self-renewal and tumor initiation capacity by functional assays. It has been also suggested that CSC populations might be responsible for chemo- and radio-therapy resistance ...
Muraro, Manuele Giuseppe
core +1 more source
, 2022 a) Spontaneous spheroid formation in BxPC3-derived subclones compared to PANC-1 cells. A total of 2000 cells seeded per well. Scale bar 100 μm. b) Live/dead labeling of spheroid cultures incubated with various concentrations of gemcitabine for 6 days ...
Ian I. Cardle (12646922) +7 more
core +1 more source
Advanced Healthcare Materials, EarlyView.Prostate patient‐derived organoids (PDOs) were established from TURP and biopsy specimens with >90% success and retain key features of their tissues of origin, making them functional avatars for antisense oligonucleotide (ASO) therapy . Transfecting single cells outside the Matrigel matrix and then reconstituting organoids overcomes the 3D delivery ...
Thi Thom Tran +15 more
wiley +1 more source
Advanced Healthcare Materials, EarlyView.A spatially controlled embedded bioprinting strategy enables precise engineering of prevascularized multicellular spheroids within skin‐derived dECM bioinks. Optimized spheroid size, composition, and spacing promote vascular maturation, paracrine signaling, and tissue integration, resulting in enhanced angiogenesis, blood perfusion, and functional skin
Minjun Ahn +6 more
wiley +1 more source
Advancing Human Skin Equivalents: The Crucial Role of Neurovascular Integration
Advanced Healthcare Materials, EarlyView.This review discusses the importance of integrating vascular and peripheral nerve systems into human skin equivalents (HSEs) to better recapitulate native skin physiology. Recent advances in vascularized, innervated, and neurovascularized HSEs are highlighted, together with emerging bioengineering strategies, current challenges, and future ...
Hao Wu +4 more
wiley +1 more source
Frontiers in Bioengineering and Biotechnology, 2020 Three-dimensional (3D) cell spheroids are being increasingly applied in many research fields due to their enhanced biological functions as compared to conventional two-dimensional (2D) cultures.
Silvia Lopa +10 more
doaj +1 more source
Characterization of spheroid cells.
, 2013 (A) The expression of CD133, CD166, Lgr5, ALDH1 and stem cell core genes Oct4/3, Sox2 and Nanog in spheroid cells were up-regulated as compared to DLD-1 adherent cells.
Hailin Xi (281719) +8 more
core +1 more source
Chemical Strategies for Multistimuli Responsive Dynamic Covalent Materials in Regenerative Medicine
Advanced Healthcare Materials, EarlyView.Multistimuli responsive dynamic covalent materials integrate reversible chemistry with the complex chemical, mechanical, and biochemical cues of regenerative environments. This review highlights chemical strategies based on orthogonal dynamic bonds, multicomponent networks, and hierarchical architectures to regulate mechanics, degradation, and ...
Saurabh Joshi +2 more
wiley +1 more source
Spheroid cells overexpressed stem cell genes.
, 2014 (A) Total RNA isolated from spheroid cells and parent cells were examined for their gene expression by a human stem cell markers cDNA array and stem cell-genes showed higher expression levels in spheroid cells to non-spheroid cells.
Kunle Odunsi (122690) +10 more
core +1 more source
Tumor spheroid characterization.
, 2023 (A) Brightfield image of the tumor spheroid and the same image with pathophysiological concentric zones. Scale bar = 100 μm (B) Fluorescent image of live/dead assay on a tumor spheroid using FDA (green) and PI (red)). Scale bar = 200 μm.
Hossein Abolhassani (14642506) +3 more
core +1 more source