Results 71 to 80 of about 60,705 (304)

Pharmacological inhibition of the PERK pathway modulates hepatocellular carcinoma growth and immune signaling

FEBS Open Bio, EarlyView.
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero, Maria Kopsida, Nathalie Arendt, Hans Lennernäs, Markus Sjöblom, Femke Heindryckx   +5 more
wiley   +1 more source

Synthesis of Two-Photon Active Tricomponent Fluorescent Probe for Distinguishment of Biotin Receptor Positive and Negative Cells and Imaging 3D-Spheroid

, 2018
A fluorescence microscopy-based distinguishment between biotin receptor (BiR) positive and negative cell lines via receptor-mediated endocytosis has been demonstrated.
Aman Sharma, Aman Sharma (565568), Kaushik Pal (2315152), Kaushik Pal, Apurba L. Koner, Apurba L. Koner (1293309)   +5 more
core   +1 more source

Spheroid formation.

, 2022
a) Spontaneous spheroid formation in BxPC3-derived subclones compared to PANC-1 cells. A total of 2000 cells seeded per well. Scale bar 100 μm. b) Live/dead labeling of spheroid cultures incubated with various concentrations of gemcitabine for 6 days ...
Ian I. Cardle (12646922), Brian J. Kirby (167953), Fredrik I. Thege (12646919), Conor N. Gruber (12646925), Sophie Cong (12646931), Megan J. Siemann (12646928), Katharina Wittmann (11990029), Justin Love (12646934)   +7 more
core   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source

Crizotinib Inhibits Viability, Migration, and Invasion by Suppressing the c-Met/PI3K/Akt Pathway in the Three-Dimensional Bladder Cancer Spheroid Model

Current Oncology
We aimed to evaluate the therapeutic potential of crizotinib, a broad-spectrum tyrosine kinase inhibitor against bladder cancer (BC) cells, based on a three-dimensional (3D) cell culture system.
Byeongdo Song, Danhyo Kim, Jin-Nyoung Ho, Van-Hung Le, Sangchul Lee   +4 more
doaj   +1 more source

Hydrostatic pressure activates HIF‐1α via β‐catenin to promote stemness in breast cancer cells

FEBS Open Bio, EarlyView.
To mimic the elevated intestinal fluid pressure in breast cancers, we loaded human breast cancer cells (MCF‐7, MDA‐MB‐453, and BT‐474) to 50 mmHg hydrostatic pressure. Hydrostatic pressure exposure upregulated HIF‐1α and induced stemness in MCF‐7 and BT‐474 cells.
Da Zhai, Yong Xu, Jingjing Ma, Ratchanee Duangrat, Chen Yan, Jingyan Liang, Hua Wei, Bin Jiang, Tao‐Sheng Li   +8 more
wiley   +1 more source

Microfluidic Biofabrication of 3D Multicellular Spheroids by Modulation of Non-geometrical Parameters

Frontiers in Bioengineering and Biotechnology, 2020
Three-dimensional (3D) cell spheroids are being increasingly applied in many research fields due to their enhanced biological functions as compared to conventional two-dimensional (2D) cultures.
Silvia Lopa, Francesco Piraino, Giuseppe Talò, Valerio Luca Mainardi, Valerio Luca Mainardi, Simone Bersini, Margherita Pierro, Luigi Zagra, Marco Rasponi, Matteo Moretti, Matteo Moretti   +10 more
doaj   +1 more source

Spheroid-3D and Monolayer-2D Intestinal Electrochemical Biosensor for Toxicity/Viability Testing: Applications in Drug Screening, Food Safety, and Environmental Pollutant Analysis

, 2019
The rise of three-dimensional cell culture systems that provide in vivo-like environments for pharmaco-toxicological models has prompted the need for simple and robust viability assays suitable for complex cell architectural structures.
Flampouri, Evangelia, O\u27Connell, Kieran, Imar, Shahzad, Evangelia Flampouri (6376373), Kieran OConnell (6376376), Shahzad Imar (1646887), Baljit Singh (40367), Singh, Baljit   +7 more
core   +1 more source

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

FEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão, Diogo M. Engrácia, Graça Soveral, Filipa Mendes   +3 more
wiley   +1 more source

Nanofabrication of Nonfouling Surfaces for Micropatterning of Cell and Microtissue

Molecules, 2010
Surface engineering techniques for cellular micropatterning are emerging as important tools to clarify the effects of the microenvironment on cellular behavior, as cells usually integrate and respond the microscale environment, such as chemical and ...
Hidenori Otsuka
doaj   +1 more source