Bub3p facilitates spindle checkpoint silencing in fission yeast [PDF]
, 2009 Although critical for spindle checkpoint signaling, the role kinetochores play in anaphase promoting complex (APC) inhibition remains unclear. Here we show that spindle checkpoint proteins are severely depleted from unattached kinetochores in fission ...
Hardwick, Kevin G. +13 more
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Aurora B suppresses microtubule dynamics and limits central spindle size by locally activating KIF4A [PDF]
, 2013 Anaphase central spindle formation is controlled by the microtubule-stabilizing factor PRC1 and the kinesin KIF4A. We show that an MKlp2-dependent pool of Aurora B at the central spindle, rather than global Aurora B activity, regulates KIF4A accumulation
Erich A. Nigg +16 more
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Dissecting the function and assembly of acentriolar microtubule organizing centers in Drosophila cells in vivo. [PDF]
PLoS Genetics, 2015 Acentriolar microtubule organizing centers (aMTOCs) are formed during meiosis and mitosis in several cell types, but their function and assembly mechanism is unclear.
Janina Baumbach +3 more
doaj +1 more source
The mechanism of spindle assembly [PDF]
The Journal of Cell Biology, 2004 Recent work has provided new insights into the mechanism of spindle assembly. Growing evidence supports a model in which the small GTPase Ran plays a central role in this process. Here, we examine the evidence for the existence of a RanGTP gradient around mitotic chromosomes and some controversial data on the role that chromosomes play in spindle ...
Oliver J. Gruss, Isabelle Vernos
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The A78V mutation in the Mad3-like domain of Schizosaccharomyces pombe Bub1p perturbs nuclear accumulation and kinetochore targeting of Bub1p, Bub3p, and Mad3p and spindle assembly checkpoint function [PDF]
, 2005 During mitosis, the spindle assembly checkpoint (SAC) responds to faulty attachments between kinetochores and the mitotic spindle by imposing a metaphase arrest until the defect is corrected, thereby preventing chromosome missegregation. A genetic screen
Vanoosthuyse, V. +6 more
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Latrunculin A delays anaphase onset in fission yeast by disrupting an ase1-independent pathway controlling mitotic spindle stability [PDF]
, 2008 It has been proposed previously that latrunculin A, an inhibitor of actin polymerization, delays the onset of anaphase by causing spindle misorientation in fission yeast.
Meadows, John C., Millar, Jonathan B. A.
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Cell & Bioscience, 2022 Background ADD1 (adducin-1) and TPX2 (targeting protein for Xklp2) are centrosomal proteins and regulate mitotic spindle assembly. Mammalian oocytes that segregate homologous chromosomes in Meiosis I and sister chromatids in Meiosis II with a spindle ...
Ying Zhang +8 more
doaj +1 more source
Spindle Assembly: Two Spindles for Two Genomes in a Mammalian Zygote [PDF]
Current Biology, 2018 A single bipolar spindle was thought to form around both parental genomes in zygotes initiating the first division. A recent study challenges this predominant view by showing that two independent spindles assemble to prevent parental genome mixing in mouse zygotes.
Al Jord, Adel, Verlhac, Marie-Hélène
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Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly [PDF]
, 2009 Mitotic spindle formation and chromosome segregation depend critically on kinetochore-microtubule (KT-MT) interactions. A new protein, termed Spindly in Drosophila and SPDL-1 in C.
Erich A. Nigg +15 more
core +1 more source
Attachment and tension in the spindle assembly checkpoint [PDF]
Journal of Cell Science, 2002 Faithful transmission of chromosomes during mitosis is ensured by the spindle assembly checkpoint. This molecular safeguard examines whether prerequisites for chromosome segregation have been satisfied and thereby determines whether to execute or to delay chromosome segregation.
Jun, Zhou, Joyce, Yao, Harish C, Joshi
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