Results 1 to 10 of about 53,406 (189)

Srsf10 and the minor spliceosome control tissue-specific and dynamic SR protein expression

eLife, 2020
Minor and major spliceosomes control splicing of distinct intron types and are thought to act largely independent of one another. SR proteins are essential splicing regulators mostly connected to the major spliceosome.
Stefan Meinke, Gesine Goldammer, A Ioana Weber, Victor Tarabykin, Alexander Neumann, Marco Preussner, Florian Heyd   +6 more
doaj   +2 more sources

Control of 3′ splice site selection by the yeast splicing factor Fyv6 [PDF]

eLife
Pre-mRNA splicing is catalyzed in two steps: 5ʹ splice site (SS) cleavage and exon ligation. A number of proteins transiently associate with spliceosomes to specifically impact these steps (first and second step factors).
Katherine A Senn, Karli A Lipinski, Natalie J Zeps, Amory F Griffin, Max E Wilkinson, Aaron A Hoskins   +5 more
doaj   +2 more sources

Non-canonical functions of spliceosome components in cancer progression

Cell Death and Disease, 2023
Dysregulation of pre-mRNA splicing is a common hallmark of cancer cells and it is associated with altered expression, localization, and mutations of the components of the splicing machinery.
O. Ivanova, Ksenia S. Anufrieva, Anastasia N. Kazakova, I. K. Malyants, P. Shnaider, M. Lukina, V. O. Shender   +6 more
semanticscholar   +1 more source

Spliceosome assembly and regulation: insights from analysis of highly reduced spliceosomes

RNA: A publication of the RNA Society, 2023
Premessenger RNA splicing is catalyzed by the spliceosome, a multimegadalton RNA–protein complex that assembles in a highly regulated process on each intronic substrate.
C. Black, T. A. Whelan, E. Garside, A. MacMillan, N. M. Fast, S. Rader   +5 more
semanticscholar   +1 more source

psiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation

Nature Communications, 2021
ATP-dependent helicases remodel the spliceosome and proofread splice site recognition. A new method – Purified Spliceosome iCLIP (psiCLIP) – probes protein-RNA interactions in defined spliceosome complexes to reveal how the helicases Prp16 and Prp22 ...
Lisa M. Strittmatter, Charlotte Capitanchik, Andrew J. Newman, Martina Hallegger, Christine M. Norman, Sebastian M. Fica, Chris Oubridge, Nicholas M. Luscombe, Jernej Ule, Kiyoshi Nagai   +9 more
doaj   +1 more source

Mutations in the splicing regulator Prp31 lead to retinal degeneration in Drosophila

Biology Open, 2021
Retinitis pigmentosa (RP) is a clinically heterogeneous disease affecting 1.6 million people worldwide. The second-largest group of genes causing autosomal dominant RP in human encodes regulators of the splicing machinery.
Sarita Hebbar, Malte Lehmann, Sarah Behrens, Catrin Hälsig, Weihua Leng, Michaela Yuan, Sylke Winkler, Elisabeth Knust   +7 more
doaj   +1 more source

Emerging roles of spliceosome in cancer and immunity

Protein & Cell, 2021
Precursor messenger RNA (pre-mRNA) splicing is catalyzed by an intricate ribonucleoprotein complex called the spliceosome. Although the spliceosome is considered to be general cell “housekeeping” machinery, mutations in core components of the spliceosome
Hui Yang, B. Beutler, Duanwu Zhang
semanticscholar   +1 more source

Targeted high throughput mutagenesis of the human spliceosome reveals its in vivo operating principles

bioRxiv, 2022
The spliceosome is a staggeringly complex machine comprising, in humans, 5 snRNAs and >150 proteins. We scaled haploid CRISPR-Cas9 base editing to target the entire human spliceosome and interrogated the mutants using the U2 snRNP/SF3b inhibitor ...
Irene Beusch, B. Rao, M. Studer, T. Luhovska, V. Šukytė, Susan Lei, J. Oses-Prieto, Em SeGraves, A. Burlingame, S. Jonas, H. Madhani   +10 more
semanticscholar   +1 more source

Elevated Small Nuclear Ribonucleoprotein Polypeptide an Expression Correlated With Poor Prognosis and Immune Infiltrates in Patients With Hepatocellular Carcinoma

Frontiers in Oncology, 2022
BackgroundElevated Small Nuclear Ribonucleoprotein Polypeptide A (SNRPA) can enhance tumor cell growth and proliferation in various cancers. However, rarely studies focus on the comprehensive analysis of SNRPA in hepatocellular carcinoma (HCC ...
Youfu Zhang, Xuyang Wang, Huaxiang Wang, Huaxiang Wang, Yi Jiang, Yi Jiang, Zhidan Xu, Laibang Luo   +7 more
doaj   +1 more source

Mechanisms of action of intranuclear microRNAs. Part 2. MicroRNA-mediated regulation of ncRNA transcriptome and alternative splicing

Zdorovʹe Rebenka, 2022
The scientific review presents the mechanisms of action of intranuclear miRNAs, namely microRNA-mediated regulation of the non-coding RNA (ncRNA) transcriptome and alternative splicing.
A.E. Abaturov, V.L. Babуch
doaj   +1 more source