An orchestrated intron retention program in meiosis controls timely usage of transcripts during germ cell differentiation [PDF]
, 2017 Global transcriptome reprogramming during sper-matogenesis ensures timely expression of factors in each phase of male germ cell differentiation. Sper-matocytes and spermatids require particularly exten-sive reprogramming of gene expression to switch from
Alberdi, Antonio J. +9 more
core +2 more sources
Modifications in small nuclear RNAs and their roles in spliceosome assembly and function
Biological chemistry, 2018 Modifications in cellular RNAs have emerged as key regulators of all aspects of gene expression, including pre-mRNA splicing. During spliceosome assembly and function, the small nuclear RNAs (snRNAs) form numerous dynamic RNA-RNA and RNA-protein ...
M. Bohnsack, K. Sloan
semanticscholar +1 more source
Identification of phenothiazine derivatives as UHM-binding inhibitors of early spliceosome assembly
Nature Communications, 2020 Interactions between U2AF homology motifs (UHMs) and U2AF ligand motifs (ULMs) play a crucial role in early spliceosome assembly in eukaryotic gene regulation.
P. Jagtap +14 more
semanticscholar +1 more source
Mutant U2AF1-expressing cells are sensitive to pharmacological modulation of the spliceosome
Nature Communications, 2017 Spliceosome mutations occur in approximately 50% of patients with myelodysplastic syndromes. Here, the authors show that tumour cells harbouring theS34F mutation in the U2AFspliceosome gene is sensitive to compounds that further perturb the spliceosome.
Cara Lunn Shirai +16 more
doaj +1 more source
Biomolecules, 2019 The spliceosome accurately promotes precursor messenger-RNA splicing by recognizing specific noncoding intronic tracts including the branch point sequence (BPS) and the 3’-splice-site (3’SS).
Jure Borišek +6 more
doaj +1 more source
The spliceosome as a new therapeutic vulnerability in aggressive prostate cancer
Molecular & Cellular Oncology, 2020 Alternative splicing (AS) analysis across the entire spectrum of human prostate cancer evolution reveals the unexpected findings that intron retention is a hallmark of stemness and tumor aggressiveness, and androgen receptor controls a splicing program ...
Cheng Zou, Dingxiao Zhang
doaj +1 more source
A bioinformatic analysis identifies circadian expression of splicing factors and time-dependent alternative splicing events in the HD-MY-Z cell line [PDF]
, 2019 The circadian clock regulates key cellular processes and its dysregulation is associated to several pathologies including cancer. Although the transcriptional regulation of gene expression by the clock machinery is well described, the role of the clock ...
Abreu, Mónica +4 more
core +1 more source
The metabolic reprogramming and vulnerability of SF3B1 mutations
Molecular & Cellular Oncology, 2020 Mutations in the splicing factor 3b subunit 1 (SF3B1) gene create a neomorphic protein that disrupts RNA splicing, but the downstream consequences of this missplicing are unclear.
W. Brian Dalton
doaj +1 more source
CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors
Genome Biology, 2019 Increasing genetic diversity via directed evolution holds great promise to accelerate trait development and crop improvement. We developed a CRISPR/Cas-based directed evolution platform in plants to evolve the rice (Oryza sativa) SF3B1 spliceosomal ...
Haroon Butt +6 more
semanticscholar +1 more source
Identification of spliceosome components pivotal to breast cancer survival
RNA Biology, 2020 Cancer cells employ alternative splicing (AS) to acquire splicing isoforms favouring their survival. However, the causes of aberrant AS in breast cancer are poorly understood. In this study, the METABRIC (Molecular Taxonomy of Breast Cancer International
Jing An +5 more
semanticscholar +1 more source