Results 111 to 120 of about 34,718 (253)

Identification of novel interacting partners of the pre-mRNA processing factor 31

, 2010
Mutations in PRPF31 (RP11 locus) cause autosomal dominant retinitis pigmentosa (adRP), an inherited disorder of the retina characterised by degeneration of rod photoreceptors. PRPF31 is ubiquitously expressed and encodes a splicing factor involved in the
Fiocco, F.
core  

Perturbation of chromatin structure globally affects localization and recruitment of splicing factors [PDF]

, 2012
Chromatin structure is an important factor in the functional coupling between transcription and mRNA processing, not only by regulating alternative splicing events, but also by contributing to exon recognition during constitutive splicing.
Risso, Guillermo, J, Risso Guillermo J., Lleres, D, Jernej Ule, Ignacio E. Schor (120406), Llères, David, Llères David, Pawellek, A., Ule, J., Kornblihtt Alberto R., Schor, Ignacio, E, Risso, GJ, Ule, Jernej, Lamond, Angus I.; id_orcid, Lamond, A.I., Alberto R. Kornblihtt (120413), Lamond, Angus, I, Kornblihtt, Alberto, R, Pawellek, Andrea, Andrea Pawellek, Alberto R Kornblihtt, David Llères (68840), Guillermo J. Risso (120408), Andrea Pawellek (120410), Risso, Guillermo J., Schor*, I.E., Schor, I.E., Lamond Angus I., Schor, Ignacio E., Ule, J, Ule Jernej, Risso, G.J., David Llères, Kornblihtt, A.R., Ignacio E Schor, Pawellek Andrea, Lleres*, D., Lamond, AI, Guillermo J Risso, Angus I Lamond, Kornblihtt, Alberto R., Llères, D., Schor Ignacio E., Pawellek, A, Schor, IE, Angus I. Lamond (13065), Kornblihtt, AR, Jernej Ule (115588)   +47 more
core   +1 more source

The spliceosome-activating complex: molecular mechanisms underlying the function of a pleiotropic regulator

Frontiers in Plant Science, 2012
Correct interpretation of the coding capacity of RNA polymerase II transcribed eukaryotic genes is determined by the recognition and removal of intronic sequences of pre-mRNAs by the spliceosome.
Csaba eKoncz, Femke edeJong, Femke edeJong, Nicolas eVillacorta, Dora eSzakonyi, Dora eSzakonyi, Zsuzsa eKoncz   +6 more
doaj   +1 more source

Proteomic Characterization of AS1411 Reveals ATP6AP1 as a Mediator of Triple‐Negative Breast Cancer Progression

PROTEOMICS, EarlyView.
ABSTRACT Triple‐negative breast cancer (TNBC) is an aggressive subtype of breast cancer characterized by high metastatic potential, poor prognosis, and limited effective therapeutic options. In this study, we investigated the molecular mechanisms underlying the anticancer effects of the nucleolin (NCL)‐targeting DNA aptamer AS1411 using label‐free ...
Ha‐Song Bae, Yun‐Jeong Seo, Eun‐Bi Kim, Hyoung‐Min Park, I‐Hsuan Liu, J. Eugene Lee, Jae‐Young Kim   +6 more
wiley   +1 more source

Postcatalytic spliceosome structure reveals mechanism of 3′–splice site selection

, 2017
Understanding splicing from the 3′ end The spliceosome removes introns from eukaryotic mRNA precursors and yields mature transcripts by joining exons.
Sebastian M. Fica, Max E. Wilkinson, Christine M. Norman, Andrew J. Newman, Kiyoshi Nagai, Wojciech P. Galej   +5 more
core   +1 more source

MTAP Deficiency as a Metabolic Vulnerability in Cancer: Implications for Synthetic Lethal Therapy

Cancer Science, EarlyView.
MTAP deletion creates a therapeutically actionable metabolic vulnerability through MTA accumulation and PRMT5 dependency. This review summarizes the biochemical basis of MTAP‐directed synthetic lethality, emerging PRMT5/MAT2A inhibitors, clinicogenomic features of MTAP‐deleted tumors, and future strategies for precision oncology.
Hiroaki Ikushima, Hidenori Kage
wiley   +1 more source

Structural studies of spliceosome assembly and catalysis

, 2019
Eukaryotic genes contain non-coding introns, removal of which during gene expression is a pre-requisite for gene function. Removal of introns and ligation of coding exons ––a process called splicing––is catalysed by a dynamic macromolecular machine ...

core   +1 more source

Disclosing the Impact of Carcinogenic SF3b Mutations on Pre-mRNA Recognition Via All-Atom Simulations

Biomolecules, 2019
The spliceosome accurately promotes precursor messenger-RNA splicing by recognizing specific noncoding intronic tracts including the branch point sequence (BPS) and the 3’-splice-site (3’SS).
Jure Borišek, Andrea Saltalamacchia, Anna Gallì, Giulia Palermo, Elisabetta Molteni, Luca Malcovati, Alessandra Magistrato   +6 more
doaj   +1 more source

Targeting the microbiota‐miRNA‐protease axis: A new therapeutic avenue in melanoma

The FEBS Journal, EarlyView.
Modulation of extracellular matrix (ECM) turnover is central to melanoma progression and metastasis, driven largely by ECM proteases. This review highlights the epigenetic regulation of ECM proteases by microRNAs and their roles in melanoma growth, invasion, and immune modulation.
Elias N. Katsoulieris, Paraskevi Ioannou, Nikolaos A. Afratis   +2 more
wiley   +1 more source

Purification of spliceosome complexes associated with the U2 snRNP.

, 2016
(A) Detection by western blot of indicated splicing factors in nuclear extracts from HeLa S3 cells either WT (NE) or stably transduced with FV5-U2-B (NEB”). (B) Comparison of the kinetic and quality of spliceosome assembly on a radiolabeled AdML pre-mRNA
Michael P. Myers (31172), Annick Harel-Bellan (448989), Eric Allemand (31170), Jose Garcia-Bernardo (3135009), Christian Muchardt (136210), Adrian R. Krainer (31173)   +5 more
core   +1 more source