Results 121 to 130 of about 34,718 (253)

Quantitative analysis of DNA‐GATA1 binding alterations linked to hematopoietic disorders

The FEBS Journal, EarlyView.
Native holdup allows the quantitative determination of affinities between full‐length transcription factors and DNA. Mutations in either the protein or the DNA can modulate binding strength, which can be precisely quantified using this approach. Applied to GATA1, it revealed mutations that alter DNA binding.
Boglarka Zambo, Bastien Morlet, Luc Negroni, Orsolya Mózner, Balázs Sarkadi, Gilles Travé, Gergo Gogl   +6 more
wiley   +1 more source

Mechanistic and structural studies of spliceosome active site formation [PDF]

, 2018
The spliceosome is a eukaryotic molecular machine composed of uridine-rich small nuclear RNAs (U snRNA) and dozens of proteins that facilitate an RNA processing event known as pre-RNA splicing.
Macrae, Andrew James
core  

The spliceosome as target for anticancer treatment

, 2009
The spliceosome is a ribonucleoprotein complex involved in RNA splicing, that is, the removal of non-coding introns from precursor messenger RNA. (Alternative) Splicing events may play an essential role in tumourigenesis.
van Alphen, RJ (Robbert), Wiemer, Erik; id_orcid, H Burger, Alphen, R.J. (Robbert) van, Eskens, Ferry, R J van Alphen, E A C Wiemer, Burger, Herman, Wiemer, E.A.C. (Erik), F A L M Eskens, Wiemer, Erik, Eskens, F.A.L.M. (Ferry), Burger, H. (Herman)   +12 more
core   +1 more source

Pentatricopeptide repeat proteins in crops: Advances in functional mechanisms and breeding applications

Journal of Integrative Plant Biology, EarlyView.
Pentatricopeptide repeat proteins coordinate nucleus‐organelle communication by modulating RNA metabolism within chloroplasts and mitochondria. This review highlights how they control critical processes like photosynthesis, seed development, fertility restoration, and stress survival in crops, and explores their potential as programmable tools for RNA ...
Mingming Wu, Maohong Cai, Rongrong Zhai, Jing Ye, Guofu Zhu, Faming Yu, Shenghai Ye, Xiaoming Zhang   +7 more
wiley   +1 more source

Tagging a Spliceosome Protein and Inhibiting Splicing in Cells [PDF]

, 2022
The spliceosome is an important molecular complex that converts pre-mRNA to mRNA. It removes non-coding regions of RNA known as introns and connects the exons through a two-step chemical reaction. The spliceosome is made up of five snRNPs and dozens of
Bredeson, Sean
core  

Modeling craniofacial spliceosomopathies: a pathway toward deciphering disease mechanisms

Frontiers in Cell and Developmental Biology
Craniofacial spliceosomopathies are syndromes resulting from mutations in components of the spliceosome, presenting with facial dysostosis in combination with other phenotypes.
Casey Griffin
doaj   +1 more source

Pangenome analysis reveals the genetic mechanism underlying high‐altitude adaptation in Qinghai–Xizang (Tibet) Plateau Rhododendron

Journal of Integrative Plant Biology, EarlyView.
Pan‐genome analysis reveals that high‐altitude Rhododendron species resist alpine cold stress by rapidly sensing and engaging the chilling response pathway and genes that directly and indirectly protect the plant from UV radiation. Heritable genomic features such as long terminal repeats contribute to the adaptive diversification of Rhododendron ...
Haoyang Zhou, Zhongping Xu, Fanhuang Zeng, Haiyu Sang, Zhenhua Liu, Miao Sun, Qiang Fu, Kaige Zhao, Daming Tan, Manzhu Bao, Shuangxia Jin, Xiuqun Liu   +11 more
wiley   +1 more source

Optimized TadA‐derived base editors efficiently manipulate mRNA splicing by A‐to‐G and C‐to‐K editing in potato

Journal of Integrative Plant Biology, EarlyView.
Two optimized TadA‐derived base editors efficiently generate diverse splicing variants by targeting specific splice sites in potato. ABSTRACT Pre‐messenger RNA (pre‐mRNA) splicing is a critical mechanism for post‐transcriptional regulation in plants. Through alternative splicing, plants produce diverse transcriptomes and proteomes that finely regulate ...
Kaiyuan Chen, Yan Zhang, Jiuzhou Deng, Lumin Zhang, Huiying Zhou, Yaxin Duan, Enle Xiao, Guangtao Zhu, Chunzhi Zhang   +8 more
wiley   +1 more source

Evaluating the Combined Effects of Elevated [CO2] and Accelerated NPQ Relaxation on Yield, Physiology and Transcription in Soybean

Plant, Cell &Environment, EarlyView.
ABSTRACT Improving photosynthesis is proposed as a means of enhancing crop productivity. To assess whether simultaneous increases in light use‐efficiency and carbon assimilation are additive, we investigated the impact of elevated CO2 on the performance of a transgenic soybean line engineered for faster relaxation of photoprotection by overexpression ...
Dilkaran Singh, Amanda P. De Souza, Lynn Doran, Atinder Singh, Jeffrey Hansen, Laurie Beth Leonelli, Steven J. Burgess   +6 more
wiley   +1 more source

Chemical treatment rescues reduced growth of the autoimmune mutant chs3‐2D without compromising its immune responses

Plant Biology, EarlyView.
We generated a compound by chemical modification that can efficiently rescue growth without decreasing the immune response of the chs3‐2D autoimmune mutants, thus chemically uncoupling its growth–defence trade‐off. Abstract Plant development in many species including Arabidopsis relies on the accurate balance between growth and defence.
M. Keijzer, M. Liebers, J. Seufer, L. Voll, C. Meier, S. Hoth   +5 more
wiley   +1 more source