Results 101 to 110 of about 53,406 (189)

The type 2C Ser/Thr phosphatase PP2C gamma is a pre-mRNA splicing factor [PDF]

, 1999
To identify activities involved in human pre-mRNA splicing, we have developed a procedure to separate HeLa cell nuclear extract into five complementing fractions.
Kobayashi, R., Krainer, A. R., Murray, M. V.   +2 more
core   +1 more source

CryoEM structure of the spliceosome immediately after branching

Nature, 2016
Precursor mRNA (pre-mRNA) splicing proceeds by two consecutive transesterification reactions via a lariat–intron intermediate. Here we present the 3.8 Å cryo-electron microscopy structure of the spliceosome immediately after lariat formation.
W. P. Galej, M. E. Wilkinson, S. Fica, C. Oubridge, A. Newman, K. Nagai   +5 more
semanticscholar   +1 more source

Global spliceosome activity regulates entry into cellular senescence

The FASEB Journal, 2020
Cellular senescence is a state of permanent growth arrest that can ultimately contribute to aging. Senescence can be induced by various stressors and is associated with a myriad of cellular functions and phenotypic markers.
S. Kwon, Seongki Min, Un-woo Jeoun, Minji Sim, G. Jung, Sun-Mi Hong, Byul A. Jee, H. Woo, Changhan Lee, G. Yoon   +9 more
semanticscholar   +1 more source

Clinical impact of splicing in neurodevelopmental disorders. [PDF]

, 2020
Clinical exome sequencing is frequently used to identify gene-disrupting variants in individuals with neurodevelopmental disorders. While splice-disrupting variants are known to contribute to these disorders, clinical interpretation of cryptic splice ...
Farh, Kyle Kai-How, Sanders, Stephan J, Schwartz, Grace B   +2 more
core   +1 more source

Identification of Five Putative Yeast RNA Helicase Genes [PDF]

, 1990
The RNA helicase gene family encodes a group of eight homologous proteins that share regions of sequence similarity. This group of evolutionarily conserved proteins presumably all utilize ATP (or some other nucleoside triphosphate) as an energy source ...
Abelson, John, Arenas, Jaime, Chang, Tien-Hsien   +2 more
core   +1 more source

Thermoconditional modulation of the pleiotropic sensitivity phenotype by the Saccharomyces cerevisiae PRP19 mutant allele pso4-1 [PDF]

, 2002
The conditionally-lethal pso4-1 mutant allele of the spliceosomal-associated PRP19 gene allowed us to study this gene’s influence on pre-mRNA processing, DNA repair and sporulation.
Bonatto, Diego, Brendel, Martin (Prof. Dr.), Cardone, Jacqueline Moraes, Feldmann, H., Grey, Martin, Henriques, Joao A. P., Revers, L. F, Saffi, Jenifer   +7 more
core  

Myelodysplastic syndrome-associated spliceosome gene mutations enhance innate immune signaling

Haematologica, 2019
Genes encoding spliceosome components including SF3B1, U2AF1 , and SRSF2 , are frequently somatically mutated in myelodysplastic syndromes (MDS), other hematologic malignancies, and solid tumors.[1][1] Typically these are mutually exclusive, heterozygous,
D. Pollyea, Chelsea Harris, Jennifer L. Rabe, Brenna R. Hedin, Lesly De Arras, Sigrid Katz, Emily C. Wheeler, R. Bejar, M. Walter, C. Jordan, E. Pietras, S. Alper   +11 more
semanticscholar   +1 more source

The nuclear GYF protein CD2BP2/U5–52K is required for T cell homeostasis

Frontiers in Immunology
The question whether interference with the ubiquitous splicing machinery can lead to cell-type specific perturbation of cellular function is addressed here by T cell specific ablation of the general U5 snRNP assembly factor CD2BP2/U5–52K.
Miriam Bertazzon, Almudena Hurtado-Pico, Carlos Plaza-Sirvent, Marc Schuster, Marco Preußner, Benno Kuropka, Fan Liu, Andor Zenon Amandus Kirsten, Xiao Jakob Schmitt, Benjamin König, Miguel Álvaro-Benito, Miguel Álvaro-Benito, Esam T. Abualrous, Esam T. Abualrous, Esam T. Abualrous, Gesa I. Albert, Stefanie Kliche, Florian Heyd, Ingo Schmitz, Christian Freund   +19 more
doaj   +1 more source

Interaction of eukaryotic translation initiation factor 4G with the nuclear cap-binding complex provides a link between nuclear and cytoplasmic functions of the m7 guanosine cap [PDF]

, 2001
In eukaryotes the majority of mRNAs have an m7G cap that is added cotranscriptionally and that plays an important role in many aspects of mRNA metabolism.
Afonina E., Boisvert F. M., Bushell M., Carmo-Fonseca M., Cho E. J., Dostie J., Dostie J., Ferreira J. A., Flaherty S. M., Fornerod M., Fortes P., Fortes P., Fraser C. S., Fromont-Racine M., Fu X. D., Gingras A. C., Gorlich D., Hentze M., Ho C. K., Izaurralde E., Izaurralde E., Jarmolowski A., Kataoka N., Kim C. Y., Kudo N., Le Hir H., Le Hir H., Lewis J. D., Lewis J. D., Lewis J. D., Lewis J. D., Martins L. M., McCracken S., Milligan J. F., Morley S. J., Morley S. J., Neugebauer K. M., Neugebauer K. M., Ohno M., Pettersson I., Piron M., Ponting C. P., Preiss T., Rozen F., Salditt-Georgieff M., Schaegger H., Shatkin A. J., Stade K., Visa N., Watanabe M., Zhou Z.   +50 more
core   +2 more sources

Spliceosome-targeted therapies trigger an antiviral immune response in triple-negative breast cancer

Cell, 2021
Elizabeth A. Bowling, Jarey H. Wang, Fade Gong, William Wu, N. Neill, I. Kim, S. Tyagi, Mayra Orellana, S. Kurley, Rocio Dominguez Vidana, Hsiang-Ching Chung, T. Hsu, Julien Dubrulle, Alexander B. Saltzman, Heyuan Li, Jitendra Kumar Meena, Gino M. Canlas, S. Chamakuri, Swarnima Singh, L. Simon, C. Olson, L. Dobrolecki, M. Lewis, Bing Zhang, I. Golding, J. Rosen, D. Young, A. Malovannaya, F. Stossi, George Miles, M. Ellis, Lihua Yu, S. Buonamici, Charles Y. Lin, Kristen L. Karlin, X. Zhang, Thomas F. Westbrook   +36 more
semanticscholar   +1 more source