Results 61 to 70 of about 58,017 (329)
Challenging the spliceosome machine [PDF]
Genome Biology, 2006 Abstract Background Using cDNA copies of transcripts and corresponding genomic sequences from the Berkeley Drosophila Genome Project, a set of 24,753 donor and acceptor splice sites were computed with a scanning algorithm that tested for single nucleotide insertion, deletion and substitution ...
Weir, Michael +2 more
openaire +2 more sources
Introns: the “dark matter” of the eukaryotic genome
Frontiers in Genetics, 2023 The emergence of introns was a significant evolutionary leap that is a major distinguishing feature between prokaryotic and eukaryotic genomes. While historically introns were regarded merely as the sequences that are removed to produce spliced ...
Kaitlin N. Girardini +4 more
doaj +1 more source
PRP4: a protein of the yeast U4/U6 small nuclear ribonucleoprotein particle [PDF]
, 1989 The Saccharomyces cerevisiae prp mutants (prp2 through prp11) are known to be defective in pre-mRNA splicing at nonpermissive temperatures. We have sequenced the PRP4 gene and shown that it encodes a 52-kilodalton protein.
Abelson, John N., Banroques, Josette
core +1 more source
Mechanism of protein-guided folding of the active site U2/U6 RNA during spliceosome activation
Science, 2020 Splicing machine shifts into gear Spliceosome activation involves extensive protein exchanges and RNA rearrangements that lead to the formation of a catalytically active U2/U6 RNA structure called Bact.
C. Townsend +9 more
semanticscholar +1 more source
A Stable Bioisostere of Ester-Linked Ubiquitin Chains Enables Decoding of Protein Interactors. [PDF]
ChembiochemAn ester‐to‐amide analog of an ester‐linked ubiquitin dimer is chemically synthesized through a convergent approach involving the sequential chemoselective ligation of three peptide fragments generated through Fmoc‐based solid‐phase peptide synthesis. The hydrolysis‐resistant ubiquitin dimer served as a chemical probe in the identification of potential
Taguchi Y +9 more
europepmc +2 more sources
Interaction of yeast eIF4G with spliceosome components Implications in pre-mRNA processing events [PDF]
, 2009 International audienceAs evidenced from mammalian cells the eukaryotic translation initiation factor eIF4G has a putative role in nuclear RNA metabolism. Here we investigate whether this role is conserved in the yeast Saccharomyces cerevisiae.
Barrass, J. David +6 more
core +2 more sources
Spliceosome Mutations in Uveal Melanoma [PDF]
International Journal of Molecular Sciences, 2020 Uveal melanoma (UM) is the most common primary intraocular malignancy of the eye. It has a high metastatic potential and mainly spreads to the liver. Genetics play a vital role in tumor classification and prognostication of UM metastatic disease. One of the driver genes mutated in metastasized UM is subunit 1 of splicing factor 3b (SF3B1), a component ...
Josephine Q.N. Nguyen +8 more
openaire +2 more sources
PLoS ONE, 2018 The spliceosome is a large ribonucleoprotein complex that catalyzes the removal of introns from RNA polymerase II-transcribed RNAs. Spliceosome assembly occurs in a stepwise manner through specific intermediates referred to as pre-spliceosome complexes E,
Erin J Vanzyl +4 more
doaj +1 more source
Journal of Developmental Biology, 2022 Mandibulofacial dysostosis (MFD) is a human congenital disorder characterized by hypoplastic neural-crest-derived craniofacial bones often associated with outer and middle ear defects.
Byung-Yong Park +4 more
doaj +1 more source
Characterization of a disease-associated mutation affecting a putative splicing regulatory element in intron 6b of the cystic fibrosis transmembrane conductance regulator (CFTR) gene [PDF]
, 2009 Cystic fibrosis (CF) is a common recessive disorder caused by >1600 mutations in the CF transmembrane conductance regulator (CFTR) gene. About 13% of CFTR mutations are classified as “splicing mutations,” but for almost 40% of these, their role in ...
BAFFICO AM +8 more
core +1 more source