Functional roles of protein splicing factors [PDF]
Bioscience Reports, 2012 RNA splicing is one of the fundamental processes in gene expression in eukaryotes. Splicing of pre-mRNA is catalysed by a large ribonucleoprotein complex called the spliceosome, which consists of five small nuclear RNAs and numerous protein factors. The spliceosome is a highly dynamic structure, assembled by sequential binding and release of the small ...
Chen, Hsin-Chou, Cheng, Soo-Chen
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PLoS Genetics, 2019 Gene expression dynamics can be measured in single living cells. Using a detectable transcriptionally active gene in living cells, we previously found that an mRNA undergoing several splicing events was retained at this gene after transcription until ...
Hodaya Hochberg-Laufer +5 more
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Alternative splicing regulation during C. elegans development: splicing factors as regulated targets. [PDF]
PLoS Genetics, 2008 Alternative splicing generates protein diversity and allows for post-transcriptional gene regulation. Estimates suggest that 10% of the genes in Caenorhabditis elegans undergo alternative splicing. We constructed a splicing-sensitive microarray to detect
Sergio Barberan-Soler, Alan M Zahler
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Splicing factor ESRP1 controls ER-positive breast cancer by altering metabolic pathways [PDF]
, 2019 The epithelial splicing regulatory proteins 1 and 2 (ESRP1 and ESRP2) control the epithelial-to-mesenchymal transition (EMT) splicing program in cancer. However, their role in breast cancer recurrence is unclear. In this study, we report that high levels
Badve, Sunil S. +9 more
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Splicing factor Prp18p promotes genome-wide fidelity of consensus 3′-splice sites [PDF]
Nucleic Acids Research, 2023 Abstract The fidelity of splice site selection is critical for proper gene expression. In particular, proper recognition of 3′-splice site (3′SS) sequences by the spliceosome is challenging considering the low complexity of the 3′SS consensus sequence YAG. Here, we show that absence of the Prp18p splicing factor results in genome-wide
Kevin R Roy +7 more
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A splicing-dependent transcriptional checkpoint associated with prespliceosome formation [PDF]
, 2014 There is good evidence for functional interactions between splicing and transcription in eukaryotes, but how and why these processes are coupled remain unknown.
Ahn +62 more
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Alternative Splicing Events and Splicing Factors Are Prognostic in Adrenocortical Carcinoma [PDF]
Frontiers in Genetics, 2020 Alternative splicing is involved in the pathogenesis of human diseases, including cancer. Here, we investigated the potential application of alternative splicing events (ASEs) and splicing factors (SFs) in the prognosis of adrenocortical carcinoma (ACC).
Jian Lv +5 more
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Human complement factor H. Two factor H proteins are derived from alternatively spliced transcripts [PDF]
, 1991 The human complement factor H is an important component in the control of the alternative pathway of complement activation. We have previously shown that at least three factor H homologous mRNA species of 4.3 kb, 1.8 kb and 1.4 kb in length are ...
Alsenz +23 more
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Regulation of splicing factors by alternative splicing and NMD is conserved between kingdoms yet evolutionarily flexible. [PDF]
, 2015 Ultraconserved elements, unusually long regions of perfect sequence identity, are found in genes encoding numerous RNA-binding proteins including arginine-serine rich (SR) splicing factors.
Brenner, Steven, Lareau, Liana
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Control of pre-mRNA splicing by the general splicing factors PUF60 and U2AF(65). [PDF]
PLoS ONE, 2007 Pre-mRNA splicing is a crucial step in gene expression, and accurate recognition of splice sites is an essential part of this process. Splice sites with weak matches to the consensus sequences are common, though it is not clear how such sites are ...
Michelle L Hastings +4 more
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