Regulation of the Stability and Localization of Post-synaptic Membrane Proteins by Liquid-Liquid Phase Separation [PDF]
Frontiers in Physiology, 2021 Synaptic plasticity is a cellular mechanism of learning and memory. The synaptic strength can be persistently upregulated or downregulated to update the information sent to the neuronal network and form a memory engram.
Tomohisa Hosokawa, Pin-Wu Liu
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Site-specific phosphorylation of PSD-95 dynamically regulates the postsynaptic density as observed by phase separation [PDF]
iScience, 2021 Summary: Postsynaptic density protein 95 is a key scaffolding protein in the postsynaptic density of excitatory glutamatergic neurons, organizing signaling complexes primarily via its three PSD-95/Discs-large/Zona occludens domains.
Maria Vistrup-Parry +10 more
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The Impact of Glutamatergic Synapse Dysfunction in the Corticothalamocortical Network on Absence Seizure Generation [PDF]
Frontiers in Molecular Neuroscience, 2022 Childhood absence epilepsy (CAE) is the most common pediatric epilepsy affecting 10–18% of all children with epilepsy. It is genetic in origin and the result of dysfunction within the corticothalamocortical (CTC) circuitry.
Beulah Leitch
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CaMKII Triggers the Diffusional Trapping of Surface AMPARs through Phosphorylation of Stargazin [PDF]
Neuron, 2010 The Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) is critically required for the synaptic recruitment of AMPA-type glutamate receptors (AMPARs) during both development and plasticity. However, the underlying mechanism is unknown. Using single-particle tracking of AMPARs, we show that CaMKII activation and postsynaptic translocation induce the ...
Patricio Opazo +6 more
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Stargazin attenuates intracellular polyamine block of calcium-permeable AMPA receptors [PDF]
Nature Neuroscience, 2007 Endogenous polyamines profoundly affect the activity of various ion channels, including that of calcium-permeable AMPA-type glutamate receptors (CP-AMPARs). Here we show that stargazin, a transmembrane AMPAR regulatory protein (TARP) known to influence transport, gating and desensitization of AMPARs, greatly reduces block of CP-AMPARs by intracellular ...
David Soto +4 more
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Slow AMPAR Synaptic Transmission Is Determined by Stargazin and Glutamate Transporters [PDF]
Neuron, 2017 AMPARs mediate the briefest synaptic currents in the brain by virtue of their rapid gating kinetics. However, at the mossy fiber-to-unipolar brush cell synapse in the cerebellum, AMPAR-mediated EPSCs last for hundreds of milliseconds, and it has been proposed that this time course reflects slow diffusion from a complex synaptic space. We show that upon
Hsin-Wei Lu +3 more
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Bidirectional Synaptic Plasticity Regulated by Phosphorylation of Stargazin-like TARPs [PDF]
Neuron, 2005 Synaptic plasticity involves protein phosphorylation cascades that alter the density of AMPA-type glutamate receptors at excitatory synapses; however, the crucial phosphorylated substrates remain uncertain. Here, we show that the AMPA receptor-associated protein stargazin is quantitatively phosphorylated and that stargazin phosphorylation promotes ...
Susumu Tomita +4 more
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Molecular Mechanism of AMPA Receptor Modulation by TARP/Stargazin [PDF]
Neuron, 2017 AMPA receptors (AMPARs) mediate the majority of fast excitatory transmission in the brain and critically contribute to synaptic plasticity and pathology. AMPAR trafficking and gating are tightly controlled by auxiliary transmembrane AMPAR regulatory proteins (TARPs).
Anat Ben-Yaacov +5 more
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Different Domains of the AMPA Receptor Direct Stargazin-mediated Trafficking and Stargazin-mediated Modulation of Kinetics [PDF]
Journal of Biological Chemistry, 2006 Stargazin is an accessory protein of AMPA receptors that enhances surface expression and also affects the biophysical properties of the receptor. AMPA receptor domains necessary for either of these two processes have not yet been identified. Here, we used confocal imaging and electrophysiology of heterologously expressed, fluorophore-tagged GluR1 ...
Matthew A. Bedoukian +2 more
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Stargazin controls the pharmacology of AMPA receptor potentiators [PDF]
Proceedings of the National Academy of Sciences, 2006 Glutamate is the major excitatory neurotransmitter in brain, and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors (AMPARs) mediate the majority of postsynaptic depolarization. AMPAR ion channels display rapid gating, and their deactivation and desensitization determine the timing of synaptic transmission.
Susumu Tomita +4 more
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