Results 31 to 40 of about 9,721 (212)

Amplification-free, highly sensitive electrochemical DNA-based sensor for simultaneous detection of stx1 and stx2 genes of Shiga toxin-producing E. coli (STEC) [PDF]

, 2022
Shiga toxin-producing E. coli (STEC) is a food-borne pathogen of significant public health concern, due to the severity of the illness it can cause and a low infection dose. The key targets in molecular-based assays to detect STEC are stx1 or stx2 genes,
Sofia Rodrigues , Teixeira, Catherine M., Burgess, Luiza, Wasiewska, Alan, O\u27Riordan, Fernando Garrido, Diaz, Geraldine, Duffy   +5 more
core   +2 more sources

High Stability of Stx2 Phage in Food and under Food-Processing Conditions [PDF]

Applied and Environmental Microbiology, 2011
ABSTRACT Bacteriophages (phages) carrying Shiga toxin genes constitute a major virulence attribute in enterohemorrhagic Escherichia coli (EHEC). Several EHEC outbreaks have been linked to food. The survival of such strains in different foods has received much attention, while the fate of the mobile Shiga ...
Tone Mari, Rode, Lars, Axelsson, Per Einar, Granum, Even, Heir, Askild, Holck, Trine M, L'abée-Lund   +5 more
openaire   +2 more sources

Virulence and extended-spectrum β-lactamase encoding genes in Escherichia coli recovered from chicken meat intended for hospitalized human consumption [PDF]

Veterinary World, 2017
Aim: This study describes the prevalence of Escherichia coli in frozen chicken meat intended for human consumption with emphasis on their virulence determinants through detection of the virulence genes and recognition of the extended-spectrum β-lactamase
Gamal A. Younis, Rasha M. Elkenany, Mohamed A. Fouda, Noura F. Mostafa   +3 more
doaj   +1 more source

Farm animal contact is associated with progression to Hemolytic uremic syndrome in patients with Shiga toxin-producing Escherichia coli — Indiana, 2012–2018

One Health, 2020
Background: Hemolytic uremic syndrome (HUS) is a life-threatening complication of Shiga toxin-producing Escherichia coli (STEC) infection. The relationship between STEC exposure and severity of clinical outcomes is not well documented.
Madhura S. Vachon, Myda Khalid, Gillian A.M. Tarr, Craig Hedberg, Jennifer A. Brown   +4 more
doaj   +1 more source

Chronological set of E. coli O157:H7 bovine strains establishes a role for repeat sequences and mobile genetic elements in genome diversification

BMC Genomics, 2020
Background Enterohemorrhagic Escherichia coli O157:H7 (EHEC) is a significant foodborne pathogen that resides asymptomatically within cattle and other ruminants.
Eliot Stanton, Taylor A. Wahlig, Dongjin Park, Charles W. Kaspar   +3 more
doaj   +1 more source

An Improved Method for the Sensitive Detection of Shiga Toxin 2 in Human Serum

Toxins, 2018
Shiga toxins (Stx) released by Stx-producing E. coli (STEC) are virulence factors that are most closely 3associated with hemolytic uremic syndrome (HUS), a life-threatening complication of intestinal infections by STEC.
Xiaohua He, Gianluigi Ardissino, Stephanie Patfield, Luisa W. Cheng, Christopher J. Silva, Maurizio Brigotti   +5 more
doaj   +1 more source

Detection of hybrid Stx1/Stx2.

, 2014
A. Using a Stx1 capture antibody and a Stx2 detection antibody, hybrid Stx1/2 molecules were detected by ELISA. Cell-free medium from mitomycin-induced STEC strains containing Stx1, Stx2, both Stx1 and Stx2, or neither was applied to the ELISA at 1/20 ...
Xiaohua He (361530), Pina Fratamico (575764), Stephanie Patfield (459595), Larry H. Stanker (37623), Craig Skinner (394507)   +4 more
core   +1 more source

Natural killer T (NKT) cells accelerate Shiga toxin type 2 (Stx2) pathology in mice

Frontiers in Microbiology, 2015
Shiga toxin-producing Escherichia coli (STEC) is a leading cause of childhood renal disease He-molytic Uremic Syndrome (HUS). The involvement of renal cytokines and chemokines is sus-pected to play a critical role in disease progression.
Fumiko eObata, Fumiko eObata, Priyanka B Subrahmanyam, Aimee E Vozenilek, Lauren M Hippler, Tynae eJeffers, Methinee eTongsuk, Irina eTiper, Progyaparamita eSaha, Dakshina M Jandhyala, Glynis L Kolling, Olga eLatinovic, Olga eLatinovic, Tonya J Webb   +13 more
doaj   +1 more source

Detection of Stx2 in culture supernatants of STEC strains.

, 2013
aStx2 subtype was determined by PCR method described previously [24]; b Signal to noise; c Results were considered Stx2-positive when signal to noise ratio >3 and Stx2-negative when signal to noise ratio ≤3; ND indicates not determined; SD indicates ...
Xiaohua He (361530), Robert E. Mandrell (37624), Reuven Rasooly (394508), Stephanie Patfield (459595), Robert Hnasko (374544)   +4 more
core   +1 more source

A challenging STEC strain isolation from patients’ stools: an O166:H15 STEC strain with the stx2 gene

Microbiology Spectrum
Two patients with acute gastroenteritis tested positive for Shiga toxin-producing Escherichia coli (STEC) by polymerase chain reaction (PCR), and both strains carried the Shiga toxin 2 encoding gene.
Surangi H. Thilakarathna, Vincent Li, Linda Chui   +2 more
doaj   +1 more source