Results 51 to 60 of about 9,721 (212)

Antibody Response to Shiga Toxins Stx2 and Stx1 in Children with Enteropathic Hemolytic-Uremic Syndrome [PDF]

Journal of Clinical Microbiology, 2001
ABSTRACT A Western blot (immunoblot) assay (WBA) for the detection of immunoglobulin G antibodies to Shiga toxins Stx2 and Stx1 in sera from 110 patients with enteropathic hemolytic-uremic syndrome (53 culture confirmed to have Shiga toxin-producing Escherichia coli [STEC] infection) and 110 age-matched ...
K, Ludwig, M A, Karmali, V, Sarkim, C, Bobrowski, M, Petric, H, Karch, D E, Müller-Wiefel   +6 more
openaire   +2 more sources

Detection of shiga toxin strains of Escherichia coli non O157 in different soft cheese by polymerase chain reaction [PDF]

Benha Veterinary Medical Journal, 2019
The objectives of the current study were detection and characterization of the isolated Escherichia coli non O157 using PCR assay. A total of 90 cheese samples high salt soft cheese, Kareish cheese and Tallaga cheese were examined for E.
Nahed Wahba, Hamdi Mohammed, Ekbal Ibrahim, Hend El barbary, Naglaa Taha   +4 more
doaj   +1 more source

Detection and Inhalation Hazard Assessment of Wear Particles Arising From Abrasion of Market‐relevant Graphene Reinforced Composites

Small, EarlyView.
Abrasion of few‐layer graphene (FLG)–epoxy composites generated respirable particles in which FLG remained fully embedded, as confirmed by correlative HR‑TEM, Raman, and ToF‑SIMS analyses. Advanced lung cell models revealed no cytotoxic or inflammatory responses, and only high‑dose epoxy particles reduced mucociliary clearance.
Govind Gupta, Jimmy Vernaz, Antje Vennemann, Maike Stange, Asmus Meyer‐Plath, Servane Contal, Sarah Zehnder, Ziting Wang, Aline Chary, Stephanie Eitner, Philipp Mueller, Vera M. Kissling, Samuel Constant, Lucie Chupin, Daniel Breitenstein, Birgit Hagenhoff, Tommaso Serchi, Bernadett Boda, Martin Wiemann, Peter Wick, Tina Buerki‐Thurnherr   +20 more
wiley   +1 more source

Structure of Shiga Toxin Type 2 (Stx2) from Escherichia coli O157:H7 [PDF]

Journal of Biological Chemistry, 2004
Several serotypes of Escherichia coli produce protein toxins closely related to Shiga toxin (Stx) from Shigella dysenteriae serotype 1. These Stx-producing E. coli cause outbreaks of hemorrhagic colitis and hemolytic uremic syndrome in humans, with the latter being more likely if the E. coli produce Stx2 than if they only produce Stx1.
Fraser, Marie E., Fujinaga, Masao, Cherney, Maia M., Melton-Celsa, Angela R., Twiddy, Edda M., O'Brien, Alison D., James, Michael N. G.   +6 more
openaire   +2 more sources

Integrated Genotypic and Phenotypic Determinants Underpin the Environmental Persistence of Escherichia coli in Treated Sewage in Japan

Environmental Microbiology Reports, Volume 18, Issue 6, December 2026.
This study integrated genotypic and phenotypic analyses to investigate the persistence of Escherichia coli in treated sewage in Japan. Virulence‐positive isolates were dominated by phylogroups D and B1, with astA and blaTEM as the predominant virulence and resistance determinants. Among accessory siderophore genes, fecA and chuA were the most prevalent.
Ida Munfarida, Jant Cres Caigoy, Christian Xedzro, Toshi Shimamoto, Tadashi Shimamoto   +4 more
wiley   +1 more source

Microvesicles containing Stx2 affected the viability and inhibited protein synthesis in CiGEnC.

, 2015
The effect of Stx2-containing microvesicles on cell viability was examined by use of a crystal violet assay (A). Data are expressed as percentage of control cell viability defined as 100%.
Ida Arvidsson (302742), Matthias Mörgelin (109607), Anne-lie Ståhl (349291), Karl E. Johansson (696336), Diana Karpman (67915), Zivile D. Békássy (370589), Milan Chromek (302739), Ann-Charlotte Kristoffersson (349289), Johan Rebetz (255622), Sebastian Loos (696337)   +9 more
core   +1 more source

Modeling Catheter‐Associated Bladder Mucosal Adhesion and Microtrauma Using a Human Urothelial Microtissue Model

Advanced NanoBiomed Research, Volume 6, Issue 10, October 2026.
Intermittent catheter use can damage the urinary tract and predispose to infection. We deployed a complex human urothelial model to investigate. Catheter contact induced compressive and shear stress causing umbrella cell disruption, reduced barrier function, inflammation and increased cell adhesion to the catheter surface.
Nazila V. Jafari, Jennifer L. Rohn
wiley   +1 more source

Evolution of the Stx2-Encoding Prophage in Persistent Bovine Escherichia coli O157:H7 Strains [PDF]

Applied and Environmental Microbiology, 2013
ABSTRACT Escherichia coli O157:H7 is a human pathogen that resides asymptomatically in its bovine host. The level of Shiga toxin (Stx) produced is variable in bovine-derived strains in contrast to human isolates that mostly produce high levels of Stx.
Dongjin, Park, Eliot, Stanton, Kristin, Ciezki, Daniel, Parrell, Matthew, Bozile, Daniel, Pike, Steven A, Forst, Kwang Cheol, Jeong, Renata, Ivanek, Dörte, Döpfer, Charles W, Kaspar   +10 more
openaire   +2 more sources

Lipopolysaccharide renders transgenic mice expressing human serum amyloid P component sensitive to Shiga toxin 2. [PDF]

PLoS ONE, 2011
Transgenic C57BL/6 mice expressing human serum amyloid P component (HuSAP) are resistant to Shiga toxin 2 (Stx2) at dosages that are lethal in HuSAP-negative wild-type mice.
Thomas P Griener, Jonathan G Strecker, Romney M Humphries, George L Mulvey, Carmen Fuentealba, Robert E W Hancock, Glen D Armstrong   +6 more
doaj   +1 more source

Characterization of six Shiga toxin-producing Escherichia coli (STEC) strains carrying Stx2-phages from Colombia

Universitas Scientiarum, 2022
Shiga toxin producing Escherichia coli (STEC) is a bacterial pathogen that causes diarrhea and severe human diseases like HUS; its main virulence factor are the Shiga toxins (Stx1 and Stx2). Some Stx2 subtypes have been identified and associated with the
Brayan Stiven Arango, Sebastián Peña Buitrago, Jhon Carlos Castaño Osorio, Claudia Viviana Granobles Velandia   +3 more
doaj   +1 more source