Results 41 to 50 of about 9,721 (212)
Neutralization of the Stx2 cytotoxic activity.
, 2013 Vero cells were incubated with a 1∶1600 dilution of cellular extracts (Panel A) or a 1∶400 dilution of culture supernatants (Panel B) derived from Vero cells transfected with pGEM-T (pGEM-T) or pStx2 (pStx2). As positive and negative controls, Vero cells
Martín Isturiz (381377) +9 more
core +1 more source
Phylogenetic analysis of Shiga toxin 1 and Shiga toxin 2 genes associated with disease outbreaks
BMC Microbiology, 2007 Background Shiga toxins 1 and 2 (Stx1 and Stx2) are bacteriophage-encoded proteins that have been associated with hemorrhagic colitis, hemolytic uremic syndrome and other severe disease conditions.
Farrell Larry D +5 more
doaj +1 more source
Immunofluorescence assay of Vero cells incubated with Stx2.
, 2013 Cells were incubated with purified Stx2 (100 CD50) or transfected with the pStx2 plasmid and stained with propidium iodide. Presence of Stx2 was detected with polyclonal anti-Stx2 serum and FITC-conjugated goat anti-mouse antibody, and analyzed by ...
Martín Isturiz (381377) +9 more
core +1 more source
Stx2 induction in SS17 and Sakai strains.
, 2015 The levels of Stx2 produced by SS17 were unaffected by induction and showed higher levels of Stx2 in uninduced cultures when compared to Sakai.
Robab Katani (690930) +12 more
core +1 more source
Characterization and zoonotic impact of Shiga toxin producing Escherichia coli in some wild bird species [PDF]
Veterinary World, 2017 Aim: Wild birds are considered silent vectors of some zoonotic water and food borne pathogens of public health significance. Owing to the importance of Shiga toxin producing Escherichia coli (STEC) as the most pathogenic among the emerging diarrheagenic ...
Hanaa Mohamed Fadel +2 more
doaj +1 more source
Advanced Science, EarlyView.A rare de novo IFT122‐A773E variant is identified in idiopathic pediatric uveitis and shown to exacerbate retinal inflammation and barrier dysfunction. Mechanistically, the variant enhances IFT43 interaction, elevates calcium signaling, and activates the MEK/ERK/FRA1 axis, revealing a previously unrecognized cilia‐associated pathway that may increase ...
Qian Zhou +18 more
wiley +1 more source
Binding of chimeric toxins of Stx2e and Stx2 to TAR.
, 2013 A) Diagram of genes encoding chimeric toxins. B) Binding of Stx2e, Stx2 and chimeric toxins to TAR (upper panel). A total of 10 μl of each TAR-binding product was loaded onto the 15% (w/v) polyacrylamide gel.
Hideyuki Arimitsu (495423) +4 more
core +1 more source
Detection of Shiga toxin produced by Escherichia coli in poultry and meat in Luxor city using multiplex PCR [PDF]
Benha Veterinary Medical Journal, 2016 Shiga toxins were widely spread in the meat especially in minced meat. These toxins have an important significance tohuman health because it is a major cause of food poisoning.
Reham Ahmed +3 more
doaj +1 more source
Advanced Science, EarlyView.Cryo‐electron microscopy structures reveal two distinct neutralization mechanisms of fully human monoclonal antibodies targeting the Shiga toxin 1a B subunit. RDS059 occludes the Gb3 receptor‐binding site, whereas RDS045 engages a lateral quaternary epitope to disrupt holotoxin assembly.
Jianting Ke +10 more
wiley +1 more source
New Stx2e Monoclonal Antibodies for Immunological Detection and Distinction of Stx2 Subtypes
PLOS ONE, 2015 Stx2e is a primary virulence factor in STEC strains that cause edema disease in neonatal piglets. Though Stx2a and Stx2e are similar, many antibody-based Stx detection kits are designed to detect Stx2a and do not recognize the Stx2e subtype.Four monoclonal antibodies against Stx2e were developed and characterized.
Craig Skinner +3 more
openaire +4 more sources