Results 271 to 280 of about 95,299 (308)

Subcellular Fractionation of Tissue Culture Cells

Current Protocols in Immunology, 2003
AbstractDevelopment of cell fractionation techniques over the last few decades has provided the means to analyze the composition and properties of purified cellular elements. In particular, subcellular fractionation is essential for the development of cell‐free assays that reconstitute complicated cellular processes.
Aniento, Fernando, Gruenberg, Jean
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BIOPOLYMERIC ASSOCIATIONS IN BACTERIAL SUBCELLULAR FRACTIONS

Canadian Journal of Microbiology, 1967
Greater amounts of intracellularly bound glucans were present in Escherichia coli, strain B/r cells, in the late lag and logarithmic growth phases than in cells in the early lag and stationary growth phases. Bound alkali-stable and alkali-labile glucose-containing polymers— as well as unbound glucans—were further characterized by their susceptibility ...
E E, Woodside, C A, Frick, C W, Fishel
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Fractionation of cells and subcellular particles with Percoll

Journal of Biochemical and Biophysical Methods, 2000
At present, centrifugation is the most common method for separation and isolation of cells and subcellular particles. The technique can be used for a wide range of applications. During latter years it has become obvious what a powerful method density gradient centrifugation is, especially when used in conjunction with sensitive assays or clinical ...
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Subcellular fractionation of brown adipose tissue

Journal of Supramolecular Structure, 1979
AbstractThe present study proposes a technique, using Metrizamide, which permits the preparation of brown adipose tissue plasma membranes from the crude mitochondria as well as from the crude microsome fraction. These plasma membranes have high relative specific activities of their marker enzyme, 5′‐nucleotidase (15 ± 3 and 14 ± 2 respectively) and ...
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Subcellular Fractionation for DIGE-Based Proteomics

2017
Mass spectrometry-based protein methodologies have revolutionized the field of analytical biochemistry and enable the identification of hundreds to thousands of proteins in biological fluids, cell lines, and tissue. This methodology requires the initial separation of a protein constellation, and this has been successfully achieved using gel-based ...
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SUBCELLULAR FRACTIONS AS TRANSPLANTATION ANTIGENS*

Annals of the New York Academy of Sciences, 1964
S, AL-ASKARI, D C, DUMONDE, H S, LAWRENCE, L, THOMAS   +3 more
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Subcellular Fractionation

2003
GEORGINA RODRÍGUEZ DE LORES ARNAIZ, AMANDA PELLEGRINO DE IRALDI   +1 more
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Damages to biological macromolecules in gonadal subcellular fractions of scallop Chlamys farreri following benzo[a]pyrene exposure: Contribution to inhibiting gonadal development and reducing fertility

Environmental Pollution, 2021
Yingying Yang, Luqing Pan, Yueyao Zhou   +2 more
exaly  

Smooth Muscle Subcellular Fractionation

1985
For a long time, anatomists recognized the existence of welldefined boundaries to cells and organelles within cells, but the techniques for subcellular fractionation for biochemical studies were not well developed until Hogeboom et al. (11, 12) published the liver cell subcellular fractionation.
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Subcellular Fractionation

Materials and Methods, 2013
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