Results 261 to 270 of about 155,734 (309)
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BIOPOLYMERIC ASSOCIATIONS IN BACTERIAL SUBCELLULAR FRACTIONS
Canadian Journal of Microbiology, 1967Greater amounts of intracellularly bound glucans were present in Escherichia coli, strain B/r cells, in the late lag and logarithmic growth phases than in cells in the early lag and stationary growth phases. Bound alkali-stable and alkali-labile glucose-containing polymers— as well as unbound glucans—were further characterized by their susceptibility ...
E E, Woodside, C A, Frick, C W, Fishel
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Isolation of subcellular fractions
1997Abstract The aim of subcellular fractionation is to separate cell organelles with as little damage as possible. The first thing to remember is that it will never be possible to separate organelles completely undamaged. In the living cell, most of the cell organelles are attached to cytoskeletal elements and are surrounded by cytosol ...
Richard H Hinton, Barbara M Mullock
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1997
Abstract Many investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers all the necessary aspects, from the homogenization of cells via a variety of separation techniques, to the isolation and characterization of peroxisomes ...
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Abstract Many investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers all the necessary aspects, from the homogenization of cells via a variety of separation techniques, to the isolation and characterization of peroxisomes ...
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Subcellular Fractionation of Human Neutrophils and Analysis of Subcellular Markers
2007The neutrophil has long been recognized for its impressive number of cytoplasmic granules that harbor proteins indispensable for innate immunity. Analysis of isolated granules has provided important information on how the neutrophil grades its response to match the challenges it meets on its passage from blood to tissues.
Lene, Udby, Niels, Borregaard
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Smooth Muscle Subcellular Fractionation
1985For a long time, anatomists recognized the existence of welldefined boundaries to cells and organelles within cells, but the techniques for subcellular fractionation for biochemical studies were not well developed until Hogeboom et al. (11, 12) published the liver cell subcellular fractionation.
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Subcellular Fractionation of Human Neutrophils and Analysis of Subcellular Markers
2014The neutrophil has long been recognized for its impressive number of cytoplasmic granules that harbor proteins indispensable for innate immunity. Analysis of isolated granules has provided important information on how the neutrophil grades its response to match the challenges it meets on its passage from blood to tissues.
Clemmensen, Stine Novrup +2 more
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Subcellular fractionation of brown adipose tissue
Journal of Supramolecular Structure, 1979AbstractThe present study proposes a technique, using Metrizamide, which permits the preparation of brown adipose tissue plasma membranes from the crude mitochondria as well as from the crude microsome fraction. These plasma membranes have high relative specific activities of their marker enzyme, 5′‐nucleotidase (15 ± 3 and 14 ± 2 respectively) and ...
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Subcellular Fractionation for DIGE-Based Proteomics
2017Mass spectrometry-based protein methodologies have revolutionized the field of analytical biochemistry and enable the identification of hundreds to thousands of proteins in biological fluids, cell lines, and tissue. This methodology requires the initial separation of a protein constellation, and this has been successfully achieved using gel-based ...
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Melanoma development from subcellular fractions.
Surgery, gynecology & obstetrics, 1979Amelanotic melanoma cells, RPMI-1846, were disrupted by sonic energy which caused cell membrane disruption, thereby allowing liberation of viable cellular organelles. These subcellular particles were injected into syngeneic Syrian and nonsyngeneic hamsters in six separate experiments to determine their possible growth potential. A sizable number of the
H S, Goldsmith, L, Stettiner
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SUBCELLULAR FRACTIONS AS TRANSPLANTATION ANTIGENS*
Annals of the New York Academy of Sciences, 1964S, AL-ASKARI +3 more
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