Results 251 to 260 of about 95,299 (308)

Sterols in yeast subcellular fractions

Lipids, 1978
AbstractYeast is the most primitive organism synthesizing substantial amounts of sterols. Because of this eucaryotic organism's versatility in growth conditions, ease of culture, well‐defined genetic mechanism, and characteristic subcellar architecture, it is readily applied to studies of the role of sterols in the general economy of the cell.
Parks, L W, McLean, Bowen C, Taylor, F R, Hough, S   +3 more
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Fractionation of Subcellular Organelles

Current Protocols in Cell Biology, 2015
AbstractThis unit provides both a theoretical and a practical background to all the techniques associated with the application of differential and density gradient centrifugation for the analysis of subcellular membranes. The density gradient information focuses on the use of the modern gradient solute iodixanol, chosen for its ease of use, versatility,
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Subcellular fractionation

1992
Abstract Subcellular fractionation has been crucial to the development of modern cellular and molecular biology. In recent years, the major driving force behind the isolation of organelles has been the requirement to study their function in vitro.
Alois Hodel, J Michael Edwardson
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Subcellular fractionation of secretory organelles

2002
Publisher Summary This chapter discusses the subcellular fractionation of secretory organelles along with a set of cell fractionation protocols used to explore whether a protein might be associated with a membrane compartment and to localize those proteins that are associated with organelles.
Chris A, Kaiser, Esther J, Chen, Sascha, Losko   +2 more
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Subcellular Fractionation of Human Neutrophils and Analysis of Subcellular Markers

2007
The neutrophil has long been recognized for its impressive number of cytoplasmic granules that harbor proteins indispensable for innate immunity. Analysis of isolated granules has provided important information on how the neutrophil grades its response to match the challenges it meets on its passage from blood to tissues.
Lene, Udby, Niels, Borregaard
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Subcellular Fractionation of Rough Microsomes

Cold Spring Harbor Protocols, 2014
When eukaryotic cells are homogenized, the rough endoplasmic reticula are converted into small vesicles, called rough microsomes. Strategies for the isolation of rough microsomes are introduced here, as are methods for evaluating the purity and intactness of an isolated rough microsomal fraction.
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Subcellular Fractionation of Human Neutrophils and Analysis of Subcellular Markers

2014
The neutrophil has long been recognized for its impressive number of cytoplasmic granules that harbor proteins indispensable for innate immunity. Analysis of isolated granules has provided important information on how the neutrophil grades its response to match the challenges it meets on its passage from blood to tissues.
Clemmensen, Stine Novrup, Udby, Lene, Borregaard, Niels   +2 more
openaire   +3 more sources

Subcellular fractionation and subcellular localization of aminopeptidase N in the rabbit enterocytes

The Journal of Membrane Biology, 1986
A fast and easy procedure is proposed for preparing concomitantly from the same sample of intestinal mucosa of A+ rabbits, four fractions high enriched in the brush-border and basolateral plasma membrane domains, rough endoplasmic reticulum, and smooth endoplasmic reticulum plus Golgi apparatus membranes, respectively.
S, Moktari, H, Feracci, J P, Gorvel, Z, Mishal, A, Rigal, S, Maroux   +5 more
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Subcellular fractionation of tissue culture cells

Trends in Biochemical Sciences, 1989
Subcellular fractionation has two major steps, (1) the homogenization of the cells and (2) the subsequent separation of the organelles. The homogenization step is discussed with reference to the problems encountered using tissue culture cells. Promising techniques for the isolation of specific compartments are illustrated using the isolation of the ...
K E, Howell, E, Devaney, J, Gruenberg
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Subcellular fractionation methods and strategies for proteomics

PROTEOMICS, 2010
Abstract Developments in subcellular fractionation strategies have provided the means to profile and analyze the protein composition of organelles and cellular structures by proteomics. Here, we review the application of classical ( e.g . density gradient centrifugation) and
Yie Hou, Lee, Hwee Tong, Tan, Maxey C M, Chung   +2 more
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