Results 251 to 260 of about 155,734 (309)

Subcellular fractionation

1992
Abstract Subcellular fractionation has been crucial to the development of modern cellular and molecular biology. In recent years, the major driving force behind the isolation of organelles has been the requirement to study their function in vitro.
Alois Hodel, J Michael Edwardson
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Subcellular Fractionation of Tissue Culture Cells

Current Protocols in Immunology, 2003
AbstractDevelopment of cell fractionation techniques over the last few decades has provided the means to analyze the composition and properties of purified cellular elements. In particular, subcellular fractionation is essential for the development of cell‐free assays that reconstitute complicated cellular processes.
Fernando, Aniento, Jean, Gruenberg
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Subcellular fractionation of secretory organelles

2002
Publisher Summary This chapter discusses the subcellular fractionation of secretory organelles along with a set of cell fractionation protocols used to explore whether a protein might be associated with a membrane compartment and to localize those proteins that are associated with organelles.
Chris A, Kaiser, Esther J, Chen, Sascha, Losko   +2 more
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Subcellular fractionation of cultured glial cells

Journal of Neurobiology, 1971
AbstractWe have studied the subcellular fractionation of a clonal line of glial cells from rat brain. In comparison to rat brain, more of the total protein is collected in the crude nuclear fraction following differential centrifugation. When a crude mitochondrial fraction is prepared and resolved on a density gradient, we find that like brain the ...
C, Cotman, H, Herschman, D, Taylor
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Subcellular fractionation of tissue culture cells

Trends in Biochemical Sciences, 1989
Subcellular fractionation has two major steps, (1) the homogenization of the cells and (2) the subsequent separation of the organelles. The homogenization step is discussed with reference to the problems encountered using tissue culture cells. Promising techniques for the isolation of specific compartments are illustrated using the isolation of the ...
K E, Howell, E, Devaney, J, Gruenberg
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Lymphocyte Stimulation by Soluble Subcellular Fractions

Tissue Antigens, 1976
Nuclear material can produce inhibition or stimulation of healthy leucocytes under different experimental conditions. Reactivity could not be produced in cultures using intact nuclei and allogeneic lymphocytes. The effect of nuclear and cytoplasm fractions was compared with that of whole cells on intact healthy lymphocytes.
G D, Pegrum, E A, Thompson, C M, Lewis, V A, Grant   +3 more
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Steroid dehydrations in porcine subcellular fractions

Steroids, 1970
Abstract Ultracentrifugation separation of porcine ovary and testis into subcellular fractions have shown that a dehydratase enzyme system is localized in the soluble cell fraction (105,000 x g.). It was further demonstrated that ovarian tissue preparations without corpora lutea were enzymatically more active than preparations from corpora lutea ...
A A, Armstrong, B, Kadis
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Subcellular Fractionation of Rough Microsomes

Cold Spring Harbor Protocols, 2014
When eukaryotic cells are homogenized, the rough endoplasmic reticula are converted into small vesicles, called rough microsomes. Strategies for the isolation of rough microsomes are introduced here, as are methods for evaluating the purity and intactness of an isolated rough microsomal fraction.
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Generation of proteoliposomes from subcellular fractions

ELECTROPHORESIS, 1997
AbstractIntracellular membranes are highly dynamic, yet they retain their identity and functional characteristics. Integral membrane proteins, which must confer this specific membrane identity, remain poorly characterized at the biochemical level, largely because detergent‐mediated solubilization is required for purification and analysis, and several ...
Rojo Ortiz De Lanzagorta, Manuel, Budin, Nicolas Philippe, Kellner, Roland, Gruenberg, Jean   +3 more
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Subcellular fractionation of mitochondria

1997
Abstract Mitochondria are ubiquitous organelles which are present in the cytoplasm of the vast majority of animal and plant cells. They are also one of the best characterized organelles and indeed were the first membrane-bound particles to be isolated in an intact functional state from living tissues. Together with chloroplasts, they are
J E Rice, J G Lindsay
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