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Detection of cardiac O-GlcNAcylation via subcellular fractionation and dual antibody analysis in pressure overload cardiac hypertrophy. [PDF]

Glycobiology
Ledee D, Zhu WZ, Olson AK.
europepmc   +1 more source

SUMOylation Protects Endothelial Cell-Expressed Leukocyte-Specific Protein 1 from Ubiquitination-Mediated Proteasomal Degradation and Facilitates Its Nuclear Export. [PDF]

Int J Mol Sci
Hossain M, Huang J, Su Y, Islam MR, Rahman MA, Cayabyab FS, Liu L.   +6 more
europepmc   +1 more source

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Subcellular fractionation of pig platelets

Biochimica et Biophysica Acta (BBA) - General Subjects, 1975
Subcellular components were obtained from pig platelets, disrupted by means of a French press and separated into 4 primary fractions. The granule fraction (10 000 g) was subjected to a sucrose gradient fractionation. Primary fractions and the granule subfractions were studied electron microscopically and biochemically by following the distribution of ...
L, Salganicoff, P A, Hebda, J, Yandrasitz, M H, Fukami   +3 more
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Subcellular fractions in rubella immunoassays

European Journal of Clinical Microbiology, 1984
Rubella virus-infected cells were fractionated by differential and sucrose gradient centrifugations. Rubella virus antigens distributed into all fractions but particulate material in the 100,000 x g pellet was shown to be enriched about two-fold for rubella virus antigen.
H, Tuokko, V, Toivonen, A, Salmi
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Subcellular Fractionation

2006
The successful combination of highly sensitive mass spectrometry and pre-fractionation techniques has provided a powerful tool to detect dynamic changes in low abundant regulatory proteins at the organelle level. Subcellular fractionation, being flexible, adjustable (both in cell and tissues), and allowing the analysis of proteins in their physiologic ...
Mariana Eça Guimarães, de Araújo, Lukas Alfons, Huber   +1 more
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Sterols in yeast subcellular fractions

Lipids, 1978
AbstractYeast is the most primitive organism synthesizing substantial amounts of sterols. Because of this eucaryotic organism's versatility in growth conditions, ease of culture, well‐defined genetic mechanism, and characteristic subcellar architecture, it is readily applied to studies of the role of sterols in the general economy of the cell.
Parks, L W, McLean, Bowen C, Taylor, F R, Hough, S   +3 more
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Subcellular fractionation of human liver

Biochimica et Biophysica Acta (BBA) - General Subjects, 1965
Abstract Human liver biopsies, obtained by a safe technique during abdominal surgery, were homogenized and subjected to subcellular fractionation by a centrifugal technique. Fractions were identified by determining appropriate tracer enzymes and other substances as well as by morphological techniques.
P, Björntorp, S, Björkerud, T, Scherstén   +2 more
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Fractionation of Subcellular Organelles

Current Protocols in Cell Biology, 2015
AbstractThis unit provides both a theoretical and a practical background to all the techniques associated with the application of differential and density gradient centrifugation for the analysis of subcellular membranes. The density gradient information focuses on the use of the modern gradient solute iodixanol, chosen for its ease of use, versatility,
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