Results 91 to 100 of about 53,984 (298)

FM1-43 reveals membrane recycling in adult inner hair cells of the mammalian cochlea [PDF]

, 2002
Neural transmission of complex sounds demands fast and sustained rates of synaptic release from the primary cochlear receptors, the inner hair cells (IHCs). The cells therefore require efficient membrane recycling.
Ashmore, JF, Griesinger, CB, Richards, CD   +2 more
core   +1 more source

Compound vesicle fusion increases quantal size and potentiates synaptic transmission [PDF]

Nature, 2009
Exocytosis at synapses involves fusion between vesicles and the plasma membrane. Although compound fusion between vesicles was proposed to occur at ribbon-type synapses, whether it exists, how it is mediated, and what role it plays at conventional synapses remain unclear.
Liming, He, Lei, Xue, Jianhua, Xu, Benjamin D, McNeil, Li, Bai, Ernestina, Melicoff, Roberto, Adachi, Ling-Gang, Wu   +7 more
openaire   +2 more sources

Nanoscale Mapping of the Subcellular Glycosylation Landscape

Advanced Science, EarlyView.
Using multiplexed super‐resolution imaging with fluorophore‐labeled lectins, this study reports intracellular glycosylation at the nanoscale across organelles and synaptic specializations. Extending glycan analysis beyond the cell surface, Glyco‐STORM reveals distinct glycosylation nanodomains in the ER, Golgi, lysosomes, and synaptic sites.
Helene Gregoria Schroeter, Steffen Sass, Mike Heilemann, Thomas Kuner, Maja Klevanski   +4 more
wiley   +1 more source

Differential sensitivity of brainstem vs cortical astrocytes to changes in pH reveals functional regional specialization of astroglia [PDF]

, 2013
Astrocytes might function as brain interoceptors capable of detecting different (chemo)sensory modalities and transmitting sensory information to the relevant neural networks controlling vital functions.
Castaldo, C, Gourine, A, Kasimov, V, Kasparov, Sergey, Larina, O, Marina, N, Patrushev, M   +6 more
core   +2 more sources

Fusion of synaptic vesicle membranes with planar bilayer membranes

Biophysical Journal, 1989
The interaction of synaptic vesicles with horizontal bilayer lipid membranes (BLMs) was investigated as a model system for neurotransmitter release. High concentrations (200 mM) of the fluorescent dye, calcein, were trapped within synaptic vesicles by freezing and thawing.
Perin, M.S., MacDonald, R.C.
openaire   +2 more sources

Spatiotemporal Regulation of Synaptic Vesicle Fusion Sites in Central Synapses [PDF]

Neuron, 2017
The number and availability of vesicle release sites at the synaptic active zone (AZ) are critical factors governing neurotransmitter release; yet, these fundamental synaptic parameters have remained undetermined. Moreover, how neural activity regulates the spatiotemporal properties of the release sites within individual central synapses is unknown ...
Dario, Maschi, Vitaly A, Klyachko
openaire   +2 more sources

Stochastic Nanoscale Biophysical Cues as a Basis for the Induction of Glioblastoma‐Like Transcriptional Programs in Astrocytes

Advanced Science, EarlyView.
Stochastic nanoscale physical cues induce glioblastoma (GBM)‐associated transcriptional traits in naïve astrocytes leading to spontaneous formation of spheroids. Cells within spheroids express activated‐MMP2 and a differential gene expression pattern involving P53 and NOTCH3, providing evidence for a role for changes in brain topography, as observed in
Laurent Starck, Tobias Butelmann, Sabrina Hogan, Melika Sarem, Bernd Heimrich, Ritwick Sawarkar, Marie‐Françoise Ritz, Gregor Hutter, V. Prasad Shastri   +8 more
wiley   +1 more source

Enzymatic Remodelling of Tumour Microenvironment Enhances Anti‐CEACAM5 CAR T‐Cell Efficacy Against Colorectal Cancer

Advanced Science, EarlyView.
This study shows anti‐CEACAM5 CAR T‐cells are ineffective against colorectal cancer (CRC) because of CEACAM5 sequestration at intercellular junctions and the thick tumour cell glycocalyx. Enzymatic treatments of CRC cell monolayer/tissue section with trypsin or hyaluronidase restore CEACAM5 availability, enhance CAR T‐cell activation, increase ...
Debasis Banik, Christopher J. Ward, Soura Chakraborty, Ziwei Zhang, Daniel Heraghty, Prasanna Suresh, Bing Li, Shekhar Kedia, Jhuma Pramanik, Simon J. Davis, James P. Roy, Michael A. Chapman, Bidesh Mahata, David Klenerman   +13 more
wiley   +1 more source

SNAP25 undergoes phase separation to facilitate the assembly of the synaptic vesicle fusion machinery

Cell Reports
Summary: The assembly of the SNARE complexes drives synaptic exocytosis in neurotransmission. SNAP25 is a key SNARE protein that pairs with syntaxin-1 and VAMP2 in synaptic vesicle fusion.
Min Zhu, Yulei Jin, Jintong Liu, Xiaoxu Kong, Yinghui Liu, Haijia Yu   +5 more
doaj   +1 more source

Nanoscale-targeted patch-clamp recordings of functional presynaptic ion channels [PDF]

, 2013
Important modulatory roles have been attributed to presynaptic NMDA receptors (NMDARs) located on cerebellar interneuron terminals. Evidence supporting a presynaptic location includes an increase in the frequency of mini events following the application ...
Benton, DCH, Caldwell, M, Moss, GWJ, Robertson, AD, Smart, TG   +4 more
core   +1 more source