Thick filament‐associated myosin undergoes frequent replacement at the tip of the thick filament [PDF]
FEBS Open Bio, 2022 Myosin plays a fundamental role in muscle contraction. Approximately 300 myosins form a bipolar thick filament, in which myosin is continuously replaced by protein turnover.
Emi Ichimura +4 more
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Thick filament mechano-sensing is a calcium-independent regulatory mechanism in skeletal muscle [PDF]
Nature Communications, 2016 Recent data suggest that muscle contraction is regulated by thick filament mechano-sensing in addition to the well-known thin filament-mediated calcium signalling pathway. Here the authors provide direct evidence that myosin activation in skeletal muscle
L. Fusi, E. Brunello, Z. Yan, M. Irving
doaj +3 more sources
Titin-mediated thick filament activation stabilizes myofibrils on the descending limb of their force–length relationship [PDF]
Journal of Sport and Health Science, 2018 Purpose: The aim of this study was to extend current half-sarcomere models by involving a recently found force-mediated activation of the thick filament and analyze the effect of this mechanosensing regulation on the length stability of half-sarcomeres ...
Gudrun Schappacher-Tilp
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Thick Filament Mechano-Sensing in Skeletal and Cardiac Muscles: A Common Mechanism Able to Adapt the Energetic Cost of the Contraction to the Task [PDF]
Frontiers in Physiology, 2018 A dual regulation of contraction operates in both skeletal and cardiac muscles. The first mechanism, based on Ca2+-dependent structural changes of the regulatory proteins in the thin filament, makes the actin sites available for binding of the myosin ...
Gabriella Piazzesi +4 more
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F-actin architecture determines constraints on myosin thick filament motion [PDF]
Nature Communications, 2022 While the impact of F-actin architecture on stress transmission is well studied, the role of architecture on stress generation remains unclear. Here authors use in vitro model and show that distinct organizations constrain myosin motion.
Camelia G. Muresan +7 more
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Contiguity and Structural Impacts of a Non-Myosin Protein within the Thick Filament Myosin Layers [PDF]
Biology, 2021 Myosin dimers arranged in layers and interspersed with non-myosin densities have been described by cryo-EM 3D reconstruction of the thick filament in Lethocerus at 5.5 Å resolution.
Lynda M. Menard +2 more
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Thick-Filament-Based Regulation and the Determinants of Force Generation [PDF]
BiomedicinesBackground/Objectives: Thick-filament-based regulation in muscle is generally conceived as processes that modulate the number of myosin heads capable of force generation.
Vivek P. Jani, Weikang Ma
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The Effects of Hsp90α1 Mutations on Myosin Thick Filament Organization. [PDF]
PLoS ONE, 2015 Heat shock protein 90α plays a key role in myosin folding and thick filament assembly in muscle cells. To assess the structure and function of Hsp90α and its potential regulation by post-translational modification, we developed a combined knockdown and ...
Qiuxia He +3 more
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Thick-Filament Extensibility in Intact Skeletal Muscle. [PDF]
Biophys J, 2018 Myofilament extensibility is a key structural parameter for interpreting myosin cross-bridge kinetics in striated muscle. Previous studies reported much higher thick-filament extensibility at low tension than the better-known and commonly used values at high tension, but in interpreting mechanical studies of muscle, a single value for thick-filament ...
Ma W +5 more
europepmc +4 more sources
Titin-mediated thick filament activation, through a mechanosensing mechanism, introduces sarcomere-length dependencies in mathematical models of rat trabecula and whole ventricle [PDF]
Scientific Reports, 2017 Recent experimental evidence in skeletal muscle demonstrated the existence of a thick-filament mechanosensing mechanism, acting as a second regulatory system for muscle contraction, in addition to calcium-mediated thin filament regulation.
Lorenzo Marcucci +2 more
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