Thick filament‐associated myosin undergoes frequent replacement at the tip of the thick filament [PDF]
FEBS Open Bio, 2022 Myosin plays a fundamental role in muscle contraction. Approximately 300 myosins form a bipolar thick filament, in which myosin is continuously replaced by protein turnover.
Emi Ichimura +4 more
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Thick-Filament-Based Regulation and the Determinants of Force Generation
BiomedicinesBackground/Objectives: Thick-filament-based regulation in muscle is generally conceived as processes that modulate the number of myosin heads capable of force generation.
Vivek P. Jani, Weikang Ma
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The Effects of Hsp90α1 Mutations on Myosin Thick Filament Organization. [PDF]
PLoS ONE, 2015 Heat shock protein 90α plays a key role in myosin folding and thick filament assembly in muscle cells. To assess the structure and function of Hsp90α and its potential regulation by post-translational modification, we developed a combined knockdown and ...
Qiuxia He +3 more
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Contiguity and Structural Impacts of a Non-Myosin Protein within the Thick Filament Myosin Layers
Biology, 2021 Myosin dimers arranged in layers and interspersed with non-myosin densities have been described by cryo-EM 3D reconstruction of the thick filament in Lethocerus at 5.5 Å resolution.
Lynda M. Menard +2 more
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Frontiers in Physiology, 2018 A dual regulation of contraction operates in both skeletal and cardiac muscles. The first mechanism, based on Ca2+-dependent structural changes of the regulatory proteins in the thin filament, makes the actin sites available for binding of the myosin ...
Gabriella Piazzesi +4 more
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Thin filament cardiomyopathies: A review of genetics, disease mechanisms, and emerging therapeutics
Frontiers in Cardiovascular Medicine, 2022 All muscle contraction occurs due to the cyclical interaction between sarcomeric thin and thick filament proteins within the myocyte. The thin filament consists of the proteins actin, tropomyosin, Troponin C, Troponin I, and Troponin T.
Lucas K. Keyt +7 more
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Sarcomere lattice geometry influences cooperative myosin binding in muscle. [PDF]
PLoS Computational Biology, 2007 In muscle, force emerges from myosin binding with actin (forming a cross-bridge). This actomyosin binding depends upon myofilament geometry, kinetics of thin-filament Ca(2+) activation, and kinetics of cross-bridge cycling.
Bertrand C W Tanner +2 more
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Myosin-based regulation of twitch and tetanic contractions in mammalian skeletal muscle
eLife, 2021 Time-resolved X-ray diffraction of isolated fast-twitch muscles of mice was used to show how structural changes in the myosin-containing thick filaments contribute to the regulation of muscle contraction, extending the previous focus on regulation by the
Cameron Hill +4 more
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Head-head interactions of resting myosin crossbridges in intact frog skeletal muscles, revealed by synchrotron x-ray fiber diffraction. [PDF]
PLoS ONE, 2012 The intensities of the myosin-based layer lines in the x-ray diffraction patterns from live resting frog skeletal muscles with full thick-thin filament overlap from which partial lattice sampling effects had been removed were analyzed to elucidate the ...
Kanji Oshima +3 more
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Force Measurements From Myofibril to Filament
Frontiers in Physiology, 2022 Contractility, the generation of force and movement by molecular motors, is the hallmark of all muscles, including striated muscle. Contractility can be studied at every level of organization from a whole animal to single molecules.
Steven Marston
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