Recovery of human gut microbiota genomes with third-generation sequencing [PDF]
Cell Death and Disease, 2021 Human gut microbiota modulates normal physiological functions, such as maintenance of barrier homeostasis and modulation of metabolism, as well as various chronic diseases including type 2 diabetes and gastrointestinal cancer. Despite decades of research,
Yanfei Li +8 more
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Application of third-generation sequencing in cancer research [PDF]
Medical Review, 2021 In the past several years, nanopore sequencing technology from Oxford Nanopore Technologies (ONT) and single-molecule real-time (SMRT) sequencing technology from Pacific BioSciences (PacBio) have become available to researchers and are currently being ...
Chen Zhiao, He Xianghuo
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Third Generation Sequencing of Epigenetic DNA.
Angewandte Chemie, 2022 The discovery of epigenetic bases has revolutionised the understanding of disease and development. Among the most studied epigenetic marks are cytosines covalently modified at the 5 position.
Bethany Searle +3 more
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MASQC: Next Generation Sequencing Assists Third Generation Sequencing for Quality Control in N6-Methyladenine DNA Identification [PDF]
Frontiers in Genetics, 2020 DNA N6-methyladenine (6mA) modification has been discovered as the most prevalent DNA modification in prokaryotes and eukaryotes, involving gene expression, DNA replication and repair, and host-pathogen interactions. Single-molecule real-time sequencing (
Siqian Yang +3 more
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3GOLD: optimized Levenshtein distance for clustering third-generation sequencing data [PDF]
BMC Bioinformatics, 2022 Background Third-generation sequencing offers some advantages over next-generation sequencing predecessors, but with the caveat of harboring a much higher error rate. Clustering-related sequences is an essential task in modern biology.
Robert Logan +6 more
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Archives of Pathology & Laboratory Medicine, 2023 CONTEXT.— Recently, new technologies, such as next-generation sequencing and third-generation sequencing, have been used in carrier screening of thalassemia. However, there is no direct comparison between the 2 methods in carrier screening of thalassemia.
Renliang Huang +10 more
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Current research status of third-generation sequencing technology in thalassemia detection [PDF]
Frontiers in PediatricsThalassemia is a hereditary hemolytic disorder primarily caused by defects in the hemoglobin genes, which impede the synthesis of hemoglobin peptide chains. This disease is mainly classified into two types: α and β.
Fenglin Zhu +3 more
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Application of third-generation sequencing technology for identifying rare α- and β-globin gene variants in a Southeast Chinese region [PDF]
BMC Medical GenomicsBackground Third-generation sequencing (TGS) based on long-read technology has been gradually used in identifying thalassemia and hemoglobin (Hb) variants.
Jianlong Zhuang +4 more
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The third generation sequencing: the advanced approach to genetic diseases
Translational Pediatrics, 2020 Genomic sequencing technologies have revolutionized mutation detection of the genetic diseases in the past few years. In recent years, the third generation sequencing (TGS) has been gaining insight into more genetic diseases owing to the single molecular
T. Xiao, Wenhao Zhou
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Diagnostic value of third-generation nanopore sequencing in extrapulmonary tuberculosis
Frontiers in Cellular and Infection MicrobiologyBackgroundThis study aimed to explore the accuracy of third-generation nanopore sequencing to diagnose extrapulmonary tuberculosis (EPTB).MethodsSamples were collected from the lesions of 67 patients with suspected EPTB admitted between April 2022 and ...
Chang Song +11 more
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