The FEBS Journal, EarlyView.The role of PARs in platelet function is fairly known; however, the underlying mechanisms are not fully understood. We report that thrombin myristoylates PAR4 in mouse platelets and PAR1 and PAR4 in human platelets, facilitating their membrane transport and interaction, leading to PAR3/4 heterodimers in mice and PAR1/4 heterodimers in humans.
Suresh Govatati +4 more
wiley +1 more source
Impact of blood centrifugation on the parameters of thrombin generation assay revisited to look for possible revision of the current guidance. [PDF]
Pract Lab MedScalambrino E +3 more
europepmc +1 more source
The FEBS Journal, EarlyView.The genes PH0137, PH0138, and PH0140 form a cluster in the genome of Pyrococcus horikoshii. The PH0140 gene encodes a transcription factor identified as a d‐amino acid‐responsive regulatory protein (DARP). In feast mode, l‐Ile‐bound DARP binds to the promoter and represses transcription of PH0137 and PH0138. In famine mode, DARP binds to d‐allo‐Ile and
Ryushi Kawakami +6 more
wiley +1 more source
The tryptophan prenyltransferase ComQ from Bacillus subtilis 168 can prenylate daptomycin at Trp1
The FEBS Journal, EarlyView.ComQ168 from Bacillus subtilis 168 catalyzes C‐terminal tryptophan farnesylation of ComX to generate a competence‐inducing pheromone. Recombinant ComQ168 also modifies cyclic peptides, including Daptomycin, likely at the N‐terminal tryptophan. Structural modeling highlights a C‐terminal binding region, supporting substrate promiscuity and establishing ...
Yanli Xu +2 more
wiley +1 more source
Thrombin Generation Assay to Support Hematologists in the Era of New Hemophilia Therapies. [PDF]
Int J Lab HematolJosset L, Rezigue H, Dargaud Y.
europepmc +1 more source
RNF13 regulates the endolysosomal pathway through interaction with the small GTPase Arl8B
The FEBS Journal, EarlyView.The E3 ubiquitin ligase RNF13 functions as a regulatory scaffold, organizing Arl8B‐dependent complexes and tuning downstream signaling that controls endolysosomal organization and trafficking. In this model, wild‐type RNF13 associates with Arl8B on endolysosomes, whereas Arl8B (E22D/F55A) or RNF13 (L244A) variants weaken complex assembly, potentially ...
Audrey M. Sénécal +4 more
wiley +1 more source
The impact of protein S and tissue factor pathway inhibitor on coagulation, assessed with thrombin generation, in women starting combined oral contraceptives. [PDF]
Res Pract Thromb HaemostStrandberg J +3 more
europepmc +1 more source
ALOX15 links lipid metabolism to receptor trafficking in platelet activation
The FEBS Journal, EarlyView.This schematic summarizes known and newly identified mechanisms of thrombin‐mediated platelet activation. In addition to classical thrombin‐induced PAR4 signaling pathways, thrombin activates an ALOX15–HETE–PKCθ–NMT1 signaling cascade that promotes protein myristoylation, facilitates receptor trafficking, and enhances PAR4 surface expression on ...
Yosef Eshetie Amare +2 more
wiley +1 more source
No correlation between thrombin generation and emicizumab levels: implications for monitoring emicizumab therapy. [PDF]
Res Pract Thromb Haemostvan der Zwet K +6 more
europepmc +1 more source
Combating ageing beyond the cell: Emerging roles of extracellular proteostasis
The FEBS Journal, EarlyView.Ageing challenges the body's ability to maintain a stable and functional proteome, leading to protein damage and aggregation both inside and outside cells. This review focuses on the less well understood mechanisms of extracellular protein quality control and how they become disrupted in ageing, particularly in neurodegenerative diseases.
Siddharth R. Venkatesh +7 more
wiley +1 more source