Results 11 to 20 of about 357,564 (302)
Therapeutic potential of siRNA-mediated transcriptional gene silencing
BioTechniques, 2006 RNA interference (RNAi) and specifically the use of small interfering RNAs (siRNAs) represents a potentially new paradigm in gene knockout technology. Clearly siRNAs can be used to knockdown the expression of a targeted transcript in what has been termed
Kevin V. Morris
doaj +3 more sources
Post-transcriptional gene silencing, transcriptional gene silencing and human immunodeficiency virus. [PDF]
World J Virol, 2015 While human immunodeficiency virus 1 (HIV-1) infection is controlled through continuous, life-long use of a combination of drugs targeting different steps of the virus cycle, HIV-1 is never completely eradicated from the body. Despite decades of research there is still no effective vaccine to prevent HIV-1 infection.
Méndez C, Ahlenstiel CL, Kelleher AD.
europepmc +4 more sources
Transcriptional gene silencing in humans. [PDF]
Nucleic Acids Res, 2016 It has been over a decade since the first observation that small non-coding RNAs can functionally modulate epigenetic states in human cells to achieve functional transcriptional gene silencing (TGS). TGS is mechanistically distinct from the RNA interference (RNAi) gene-silencing pathway.
Weinberg MS, Morris KV.
europepmc +5 more sources
Antisense transcription licenses nascent transcripts to mediate transcriptional gene silencing. [PDF]
Genes Dev, 2016 In eukaryotes, antisense transcription can regulate sense transcription by induction of epigenetic modifications. We showed previously that antisense transcription triggers Dicer-independent siRNA (disiRNA) production and disiRNA locus DNA methylation (DLDM) in Neurospora crassa.
Dang Y, Cheng J, Sun X, Zhou Z, Liu Y.
europepmc +4 more sources
Virus‐Induced Gene Silencing, a Post Transcriptional Gene Silencing Method [PDF]
International Journal of Plant Genomics, 2009 Virus‐induced gene silencing (VIGS) is one of the reverse genetics tools for analysis of gene function that uses viral vectors carrying a target gene fragment to produce dsRNA which trigger RNA‐mediated gene silencing. There are a number of viruses which have been modified to silence the gene of interest effectively with a sequence‐specific manner ...
Unver, Turgay, Budak, Hikmet
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Characteristics of post‐transcriptional gene silencing [PDF]
EMBO reports, 2001 A number of gene silencing phenomena that inactivate genes at the post‐transcriptional level have been identified. Due to its potential for studying gene function, post‐transcriptional gene silencing (PTGS) has become an intense area of research. In this review we describe the different means of inducing PTGS and discuss the possible biological roles ...
A. Chicas, MACINO, Giuseppe
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Assessment of RNAi-induced silencing in banana (Musa spp.) [PDF]
, 2015 In plants, RNA- based gene silencing mediated by small RNAs functions at the transcriptional or post-transcriptional level to negatively regulate target genes, repetitive sequences, viral RNAs and/or transposon elements.
Cammue, B.P.A. +6 more
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Epigenetic silencing in transgenic plants
Frontiers in Plant Science, 2015 Epigenetic silencing is a natural phenomenon in which the expression of gene is regulated through modifications of DNA, RNA or histone proteins. It is a mechanism for defending host genomes against the effects of transposable element, viral infection and
Sarma eRajeev Kumar +2 more
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Traffic into silence: endomembranes and post-transcriptional RNA silencing. [PDF]
, 2014 microRNAs (miRNAs) and small interfering RNAs (siRNAs) are small RNAs that repress gene expression at the post-transcriptional level in plants and animals. Small RNAs guide Argonaute-containing RNA-induced silencing complexes to target RNAs in a sequence-
Chen, Xuemei +2 more
core +3 more sources
Internuclear gene silencing in Phytophthora infestans is established through chromatin remodelling [PDF]
, 2008 In the plant pathogen Phytophthora infestans, nuclear integration of inf1 transgenic DNA sequences results in internuclear gene silencing of inf1. Although silencing is regulated at the transcriptional level, it also affects transcription from other ...
Appiah, A.A. +7 more
core +3 more sources