Results 21 to 30 of about 1,124,626 (332)

Bidirectional transcription directs both transcriptional gene activation and suppression in human cells. [PDF]

open access: yesPLoS Genetics, 2008
Small RNAs targeted to gene promoters in human cells have been shown to modulate both transcriptional gene suppression and activation. However, the mechanism involved in transcriptional activation has remained poorly defined, and an endogenous RNA ...
Kevin V Morris   +4 more
doaj   +1 more source

Virus‐Induced Gene Silencing, a Post Transcriptional Gene Silencing Method [PDF]

open access: yesInternational Journal of Plant Genomics, 2009
Virus‐induced gene silencing (VIGS) is one of the reverse genetics tools for analysis of gene function that uses viral vectors carrying a target gene fragment to produce dsRNA which trigger RNA‐mediated gene silencing. There are a number of viruses which have been modified to silence the gene of interest effectively with a sequence‐specific manner ...
Unver, Turgay, Budak, Hikmet
openaire   +4 more sources

Transgene-induced gene silencing is not affected by a change in ploidy level. [PDF]

open access: yesPLoS ONE, 2008
BACKGROUND: Whole genome duplication, which results in polyploidy, is a common feature of plant populations and a recurring event in the evolution of flowering plants.
Daniela Pignatta   +4 more
doaj   +1 more source

Silencing of a plant gene by transcriptional interference [PDF]

open access: yesNucleic Acids Research, 2009
Integration of foreign DNA into eukaryotic genomes results frequently in a total or partial loss of gene function, caused by the interruption of indispensable structures of the gene itself. Using T-DNA insertions in Arabidopsis we screened for mutants with deregulated chlorophyll precursor accumulation in etiolated seedlings.
Hedtke, Boris, Grimm, Bernhard
openaire   +2 more sources

Global Analysis of RNA-Dependent RNA Polymerase-Dependent Small RNAs Reveals New Substrates and Functions for These Proteins and SGS3 in Arabidopsis

open access: yesNon-Coding RNA, 2021
RNA silencing pathways control eukaryotic gene expression transcriptionally or posttranscriptionally in a sequence-specific manner. In RNA silencing, the production of double-stranded RNA (dsRNA) gives rise to various classes of 20–24 nucleotide (nt ...
Xia Hua   +5 more
doaj   +1 more source

Endogenous Targets of Transcriptional Gene Silencing in Arabidopsis [PDF]

open access: yesThe Plant Cell, 2000
Transcriptional gene silencing (TGS) frequently inactivates foreign genes integrated into plant genomes but very likely also suppresses an unknown subset of chromosomal information. Accordingly, RNA analysis of mutants impaired in silencing should uncover endogenous targets of this epigenetic regulation.
Steimer, A.   +5 more
openaire   +5 more sources

Transcriptional Silencing of Geminiviral Promoter-Driven Transgenes Following Homologous Virus Infection

open access: yesMolecular Plant-Microbe Interactions, 2003
Promoters isolated from the Tomato leaf curl virus (TLCV) drive both constitutive and tissue-specific expression in transgenic tobacco. Following systemic TLCV infection of plants stably expressing TLCV promoter:GUS transgenes, transgene expression ...
Mark Seemanpillai   +3 more
doaj   +1 more source

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