Results 21 to 30 of about 5,670,610 (287)

Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing. [PDF]

Cell, 2021
SUMMARY A general approach for heritably altering gene expression has the potential to enable many discovery and therapeutic efforts. Here, we present CRISPRoff—a programmable epigenetic memory writer consisting of a single dead Cas9 fusion protein that ...
Nuñez JK, Chen J, Pommier GC, Cogan JZ, Replogle JM, Adriaens C, Ramadoss GN, Shi Q, Hung KL, Samelson AJ, Pogson AN, Kim JYS, Chung A, Leonetti MD, Chang HY, Kampmann M, Bernstein BE, Hovestadt V, Gilbert LA, Weissman JS.   +19 more
europepmc   +2 more sources

Matrix mechano-sensing at the invasive front induces a cytoskeletal and transcriptional memory supporting metastasis. [PDF]

Nat Commun
The extracellular matrix (ECM) controls tumour dissemination. We characterise ECM organization in human and mouse tumours, identifying three regions: tumour body, proximal invasive front and distal invasive front.
Maiques O, Sallan MC, Laddach R, Pandya P, Varela A, Crosas-Molist E, Barcelo J, Courbot O, Liu Y, Graziani V, Arafat Y, Sewell J, Rodriguez-Hernandez I, Fanshawe B, Jung-Garcia Y, Imbert PR, Grasset EM, Albrengues J, Santacana M, Macià A, Tarragona J, Matias-Guiu X, Marti RM, Tsoka S, Gaggioli C, Orgaz JL, Fruhwirth GO, Wallberg F, Betteridge K, Reyes-Aldasoro CC, Haider S, Braun A, Karagiannis SN, Elosegui-Artola A, Sanz-Moreno V.   +34 more
europepmc   +2 more sources

CRISPR-dCas13-tracing reveals transcriptional memory and limited mRNA export in developing zebrafish embryos. [PDF]

Genome Biol, 2023
Background Understanding gene transcription and mRNA-protein (mRNP) dynamics in single cells in a multicellular organism has been challenging. The catalytically dead CRISPR-Cas13 (dCas13) system has been used to visualize RNAs in live cells without ...
Huang Y, Gao BQ, Meng Q, Yang LZ, Ma XK, Wu H, Pan YH, Yang L, Li D, Chen LL.   +9 more
europepmc   +2 more sources

Transcriptional memory of gene expression across generations participates in transgenerational plasticity of field pennycress in response to cadmium stress [PDF]

Frontiers in Plant Science, 2022
Transgenerational plasticity (TGP) occurs when maternal environments influence the expression of traits in offspring, and in some cases may increase fitness of offspring and have evolutionary significance.
Gengyun Li, Gengyun Li, Yuewan Zhao, Fei Liu, Minnuo Shi, Yabin Guan, Ticao Zhang, Fangqing Zhao, Fangqing Zhao, Qin Qiao, Qin Qiao, Yupeng Geng   +11 more
doaj   +2 more sources

Transcriptional memory and response to adverse temperatures in plants. [PDF]

J Zhejiang Univ Sci B, 2021
Temperature is one of the major environmental signals controlling plant development, geographical distribution, and seasonal behavior. Plants perceive adverse temperatures, such as high, low, and freezing temperatures, as stressful signals that can cause
Xie W, Tang Q, Yan F, Tao Z.
europepmc   +2 more sources

Heteromeric HSFA2/HSFA3 complexes drive transcriptional memory after heat stress in Arabidopsis. [PDF]

Nat Commun, 2021
Adaptive plasticity in stress responses is a key element of plant survival strategies. For instance, moderate heat stress (HS) primes a plant to acquire thermotolerance, which allows subsequent survival of more severe HS conditions.
Friedrich T, Oberkofler V, Trindade I, Altmann S, Brzezinka K, Lämke J, Gorka M, Kappel C, Sokolowska E, Skirycz A, Graf A, Bäurle I.   +11 more
europepmc   +2 more sources

Transcriptional Memory in the Drosophila Embryo [PDF]

Current Biology, 2016
International audienceTransmission of active transcriptional states from mother to daughter cells has the potential to foster precision in the gene expression programs underlying development.
Lagha, Mounia, Esposito, Emilia, Coppey, Mathieu, Walczak, Aleksandra M, Mancini, Laure, Levine, Michael, Ferraro, Teresa, Ng, Sam, Lucas, Tanguy, Dostatni, Nathalie, Walczak, Aleksandra m.   +10 more
core   +4 more sources

INO1 transcriptional memory leads to DNA zip code-dependent interchromosomal clustering

Microbial Cell, 2015
Many genes localize at the nuclear periphery through physical interaction with the nuclear pore complex (NPC). We have found that the yeast INO1 gene is targeted to the NPC both upon activation and for several generations after repression, a phenomenon ...
Donna Garvey Brickner, Robert Coukos, Jason H. Brickner   +2 more
doaj   +2 more sources

Oncogenic enhancers prime quiescent metastatic cells to escape NK immune surveillance by eliciting transcriptional memory. [PDF]

Nat Commun
Metastasis arises from disseminated tumour cells (DTCs) that are characterized by intrinsic phenotypic plasticity and the capability of seeding to secondary organs. DTCs can remain latent for years before giving rise to symptomatic overt metastasis.
Michelatti D, Beyes S, Bernardis C, Negri ML, Morelli L, Bediaga NG, Poli V, Fagnocchi L, Lago S, D'Annunzio S, Cona N, Gaspardo I, Bianchi A, Jovetic J, Gianesello M, Turdo A, D'Accardo C, Gaggianesi M, Dori M, Forcato M, Crispatzu G, Rada-Iglesias A, Sosa MS, Timmers HTM, Bicciato S, Todaro M, Tiberi L, Zippo A.   +27 more
europepmc   +2 more sources

Transcriptional Memory in Taraxacum mongolicum in Response to Long-Term Different Grazing Intensities [PDF]

Plants, 2022
Grazing, as an important land use method in grassland, has a significant impact on the morphological and physiological traits of plants. However, little is known about how the molecular mechanism of plant responds to different grazing intensities.
Yalin Wang, Wenyan Zhu, Fei Ren, Na Zhao, Shixiao Xu, Ping Sun   +5 more
doaj   +2 more sources