Results 171 to 180 of about 1,138 (192)
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Transgalactosylation activity of sweet almond α-galactosidase: Synthesis of saccharides
Phytochemistry, 1979Abstract Sweet almond α-galactosidase (α- d -galactoside galactohydrolase, EC 3.2.1.22) catalyses hydrolytic, synthetic ( de novo ) and transfer reactions. Transfer products were formed using p -nitrophenyl α- d -galactoside as the galactosyl donor and glucose, galactose, sucrose, maltose and lactose as acceptors; several of the products were ...
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Applied Microbiology and Biotechnology, 2002
A beta-galactosidase isoenzyme, beta-Gall, from Bifidobacterium infantis HL96, was expressed in Escherichia coli and purified to homogeneity. The molecular mass of the beta-Gall subunit was estimated to be 115 kDa by SDS-PAGE. The enzyme appeared to be a tetramer, with a molecular weight of about 470 kDa by native PAGE.
M-N, Hung, B H, Lee
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A beta-galactosidase isoenzyme, beta-Gall, from Bifidobacterium infantis HL96, was expressed in Escherichia coli and purified to homogeneity. The molecular mass of the beta-Gall subunit was estimated to be 115 kDa by SDS-PAGE. The enzyme appeared to be a tetramer, with a molecular weight of about 470 kDa by native PAGE.
M-N, Hung, B H, Lee
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Enzyme and Microbial Technology, 1990
Abstract β- d -Galactoside galactohydrolase (E.C. 3.2.1.23) was chemically modified with 1,1′-carbonyldiimidazole-activated polyethylene glycols (MW 2,000, 8,000, and 20,000). The modified β-galactosidases had over 50% of amino groups coupled to polyethylene glycol but retained over 50% of the original activity.
Jean E. Beecher +2 more
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Abstract β- d -Galactoside galactohydrolase (E.C. 3.2.1.23) was chemically modified with 1,1′-carbonyldiimidazole-activated polyethylene glycols (MW 2,000, 8,000, and 20,000). The modified β-galactosidases had over 50% of amino groups coupled to polyethylene glycol but retained over 50% of the original activity.
Jean E. Beecher +2 more
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International Journal of Biological Macromolecules
β-Galactosidases are highly desirable in various biotechnological applications. However, research on those obtained from Klebsiella strains has been noticeably restricted. The present investigation centers on the isolation, purification, and characterization of a β-galactosidase enzyme derived from Klebsiella michiganensis (GALB5582Y).
Aicha, Chenafa +5 more
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β-Galactosidases are highly desirable in various biotechnological applications. However, research on those obtained from Klebsiella strains has been noticeably restricted. The present investigation centers on the isolation, purification, and characterization of a β-galactosidase enzyme derived from Klebsiella michiganensis (GALB5582Y).
Aicha, Chenafa +5 more
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International Dairy Journal, 2018
Abstract Raw transgalactosylated oligosaccharides (raw TOS) obtained by enzymatic synthesis with Aspergillus oryzae β-galactosidase were purified by selective bioconversion with Saccharomyces cerevisiae ATCC4126 and Kluyveromyces marxianus NRLLY-1109. Monosaccharides were completely removed by S. cerevisiae and K.
Cecilia Guerrero +2 more
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Abstract Raw transgalactosylated oligosaccharides (raw TOS) obtained by enzymatic synthesis with Aspergillus oryzae β-galactosidase were purified by selective bioconversion with Saccharomyces cerevisiae ATCC4126 and Kluyveromyces marxianus NRLLY-1109. Monosaccharides were completely removed by S. cerevisiae and K.
Cecilia Guerrero +2 more
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Reversible Transgalactosylation1
Journal of the American Chemical Society, 1958John H. Pazur +2 more
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