Engineering and application of LacI mutants with stringent expressions [PDF]
Microbial BiotechnologyOptimal transcriptional regulatory circuits are expected to exhibit stringent control, maintaining silence in the absence of inducers while exhibiting a broad induction dynamic range upon the addition of effectors.
Jieyuan Wu +9 more
doaj +3 more sources
One-Pot Enzymatic Bioconversion of Native Whey for the Simultaneous Production of Galacto-Oligosaccharides and Antioxidant Peptides [PDF]
FoodsThe integrated valorization of whey into multifunctional food ingredients is constrained by sequential processing routes and the need for purified lactose and protein fractions.
Andrés Córdova-Suárez +11 more
doaj +2 more sources
A New β-Galactosidase from the Antarctic Bacterium Alteromonas sp. ANT48 and Its Potential in Formation of Prebiotic Galacto-Oligosaccharides [PDF]
Marine Drugs, 2019 As an important medical enzyme, β-galactosidases catalyze transgalactosylation to form prebiotic Galacto-Oligosaccharides (GOS) that assist in improving the effect of intestinal flora on human health.
Shangyong Li, Xiangjie Zhu, Mengxin Xing
doaj +2 more sources
Galacto-oligosaccharide production by Lactobacillus delbrueckii ssp. bulgaricus whole cells and lysates [PDF]
JDS Communicationsβ-Galactosidase is currently applied in foods for reduction of lactose but can also be used for its transgalactosylation activity to synthesize galacto-oligosaccharides (GOS) as prebiotics.
Giselle K.P. Guron +4 more
doaj +2 more sources
Microwave-Assisted Synthesis of Glycoconjugates by Transgalactosylation with Recombinant Thermostable β-Glycosidase from Pyrococcus [PDF]
International Journal of Molecular Sciences, 2016 The potential of the hyperthermophilic β-glycosidase from Pyrococcus woesei (DSM 3773) for the synthesis of glycosides under microwave irradiation (MWI) at low temperatures was investigated. Transgalactosylation reactions with β-N-acetyl-d-glucosamine as acceptor substrate (GlcNAc-linker-tBoc) under thermal heating (TH, 85 °C) and under MWI at 100 and ...
Dorothee Merker, Lothar Elling
exaly +4 more sources
Study of the transgalactosylation activity of ß-galactosidase from a new strain Kluyveromyces lactis 3 [PDF]
Journal of BioScience and Biotechnology, 2012 Beta-galactosidase (EC.3.2.1.23) is an important enzyme industrially used for the hydrolysis of lactose from milk and milk whey for several applications.
ILIA ILIEV, TONKA VASILEVA
doaj +2 more sources
Chemical Engineering Transactions, 2014 A small-scale EMR system was developed with a reliable control design giving the possibility for continuous operation at constant flux (constant hydraulic residence time, tHRT).
A.B. Sitanggang, A. Drews, M. Kraume
doaj +2 more sources
Enzymatic Building-Block Synthesis for Solid-Phase Automated Glycan Assembly. [PDF]
Angew Chem Int Ed Engl, 2020 In a chemoenzymatic approach to oligosaccharide synthesis, the scalability potential of enzymatic synthesis was exploited for the preparative synthesis of a relevant N‐acetyllactosamine disaccharide building block. This disaccharide was then applied successfully to the automated synthesis of a target oligosaccharide using the glycan synthesizer ...
Marchesi A +7 more
europepmc +3 more sources
A GH42 β‑Galactosidase from the Human Isolate Bifidobacterium breve DSM 20213: Biochemical and Transgalactosylation Properties Reveal the Potential for Galacto-oligosaccharides Synthesis [PDF]
ACS OmegaKhanh-Trang Vu-Le +9 more
doaj +2 more sources
Journal of Dairy Science, 2022 : β-Galactosidase is one of the most important enzymes used in dairy processing. It converts lactose into glucose and galactose, and also catalyzes galactose to form galactooligosaccharides (GOS), so-called prebiotics.
J.C. Zhao +5 more
doaj +1 more source