Recombinant microbial systems for improved β-galactosidase production and biotechnological applications [PDF]
, 2011 β-Galactosidases (EC 3.2.1.23) constitute a large family of proteins that are known to catalyze both hydrolytic and transgalactosylation reactions. The hydrolytic activity has been applied in the food industry for decades for reducing the lactose content
Domingues, Lucília +2 more
core +1 more source
Annals of Microbiology, 2021 Purpose Gracilibacillus dipsosauri strain DD1 is a salt-tolerant Gram-positive bacterium that can hydrolyze the synthetic substrates o-nitrophenyl-β-d-galactopyranoside (β-ONP-galactose) and p-nitrophenyl-α-d-galactopyranoside (α-PNP-galactose).
Charles E. Deutch +2 more
doaj +1 more source
Biocatalytic approaches using lactulose: end product compared with substrate [PDF]
, 2016 Lactulose is a lactose-based carbohydrate with well-known prebiotic effect and recognized medical applications. Currently, the commercially available lactulose is chemically synthesized. Nevertheless, the process leads to low yields and high levels of by-
Macedo, Eugénia A. +3 more
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Biochemical characterization of three Aspergillus niger β-galactosidases
Electronic Journal of Biotechnology, 2017 Background: β-Galactosidases catalyze both hydrolytic and transgalactosylation reactions and therefore have many applications in food, medical, and biotechnological fields.
Dandan Niu +7 more
doaj +1 more source
Continuous Packed Bed Reactor with Immobilized β-Galactosidase for Production of Galactooligosaccharides (GOS) [PDF]
, 2017 The β-galactosidase from Bacillus circulans was covalently attached to aldehyde-activated (glyoxal) agarose beads and assayed for the continuous production of galactooligosaccharides (GOS) in a packed-bed reactor (PBR).
Ballesteros Olmo, Antonio +4 more
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Galactooligosaccharides production by β-galactosidase immobilized onto magnetic polysiloxane–polyaniline particles [PDF]
, 2009 Magnetized polysiloxane coated with polyaniline (mPOS–PANI) was used as a support for β-galactosidase immobilization via glutaraldehyde. The galactooligosaccharides (GOS) production by this derivative was investigated under different initial lactose ...
Balcão, V. M. +5 more
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UV spectrophotometry method for the monitoring of galacto-oligosaccharides production [PDF]
, 2009 Monitoring the industrial production of galacto-oligosaccharides (GOS) requires a fast and accurate methodology able to quantify, in real time, the substrate level and the product yield.
Correia, Daniela M. +7 more
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Galactooligosaccharides: production, health benefits, application to foods and perspectives
Scientia Agropecuaria, 2015 Synthesized from lactose transgalactosylation, galactooligosaccharides are non-digestible carbohydrates classified as prebiotic ingredients of high added value.
Ana Elizabeth Cavalcante Fai +1 more
doaj +1 more source
Galacto-oligosaccharides production during lactose hydrolysis by free Aspergillus oryzae β-galactosidase and immobilized on magnetic polysiloxane-polyvinyl alcohol [PDF]
, 2009 The synthesis of galacto-oligosaccharides (GOS) by the action of Aspergillus oryzae β-galactosidase free and immobilized on magnetic polysiloxane-polyvinyl alcohol (mPOS-PVA) was studied.
Balcão, V. M. +8 more
core +1 more source
Transgalactosylation activity of ebg beta-galactosidase synthesizes allolactose from lactose [PDF]
Journal of Bacteriology, 1982 ebg enzyme, the second beta-galactosidase of Escherichia coli, does not normally convert lactose into an inducer of the lac operon. We previously reported the existence of a mutant ebg enzyme that does make such an inducer in vivo (Rolseth et al., J. Bacteriol. 142:1036-1039, 1980).
openaire +2 more sources