Results 51 to 60 of about 1,138 (192)

Production of high‐purity galacto‐oligosaccharides by depleting glucose and lactose from galacto‐oligosaccharide syrup with yeasts

Yeast, Volume 37, Issue 9-10, Page 515-530, September-October 2020., 2020
Abstract Galacto‐oligosaccharides (GOS) are prebiotic compounds, widely used as ingredients in various food, nutraceutical and pharmaceutical products. Enzymatic synthesis of GOS results in low‐purity products that contain high amounts of glucose and lactose beside the valuable GOS.
Melinda Pázmándi, Zoltán Kovács, Edina Balga, Mónika Kovács, Anna Maráz   +4 more
wiley   +1 more source

Isolation and selection of producing fungi β-galactosidase [PDF]

Evidência, 2012
The micro-organisms represent the greatest biological diversity, chemistry, molecular and physiological ofliving and isolation from natural resources such as soil, is of great importance for obtaining new strains.The objective of this study was to ...
Lilian Cristina Lopes Schuber et al.
doaj  

Heterologous Expression of a Thermostable β-1,3-Galactosidase and Its Potential in Synthesis of Galactooligosaccharides

Marine Drugs, 2018
A thermostable β-1,3-galactosidase from Marinomonas sp. BSi20414 was successfully heterologously expressed in Escherichia coli BL21 (DE3), with optimum over-expression conditions as follows: the recombinant cells were induced by adding 0.1 mM of ...
Haitao Ding, Lili Zhou, Qian Zeng, Yong Yu, Bo Chen   +4 more
doaj   +1 more source

Two β-galactosidases from the human isolate Bifidobacterium breve DSM 20213: molecular cloning and expression, biochemical characterization and synthesis of galacto-oligosaccharides.

PLoS ONE, 2014
Two β-galactosidases, β-gal I and β-gal II, from Bifidobacterium breve DSM 20213, which was isolated from the intestine of an infant, were overexpressed in Escherichia coli with co-expression of the chaperones GroEL/GroES, purified to electrophoretic ...
Sheryl Lozel Arreola, Montira Intanon, Jasmina Suljic, Roman Kittl, Ngoc Hung Pham, Paul Kosma, Dietmar Haltrich, Thu-Ha Nguyen   +7 more
doaj   +1 more source

Síntese de galacto-oligossacarídeos a partir de lactose usando β-galactosidase comercial de Kluyveromyces lactis Synthesis of galactooligosaccharides from lactose using commercial β-galactosidase from Kluyveromyces lactis

Brazilian Journal of Food Technology, 2012
O objetivo deste trabalho foi estudar a influência de parâmetros reacionais na obtenção via enzimática de galacto-oligossacarídeos (GOS), utilizando-se a enzima comercial β-galactosidase de Kluyveromyces lactis (Lactozym® 3000 L), tendo como ...
Cristiane Reinaldo Lisboa, Fátima Aparecida de Almeida Costa, Janaína Fernandes de Medeiros Burkert, Carlos André Veiga Burkert   +3 more
doaj   +1 more source

Optimal Production of β-Galactosidase from Lactobacillus fermentum for the Synthesis of Prebiotic Galactooligosaccharides (Gos)

Journal of Pure and Applied Microbiology, 2020
The enzyme β-galactosidase (β-gal) has extensively used for improvement of lactose intolerance condition. Present study, was designed to assess the potential of β-gal enzyme produced by Lactobacillus fermentum, a kefir isolate, as a biocatalyst for the ...
Shilpa Mahadevaiah, Renuka Basavaiah, Manmohan Parida, Harsh Vardhan Batra   +3 more
doaj   +1 more source

Stability of oligosaccharides derived from lactulose during the processing of milk and apple juice [PDF]

, 1994
The scientific evidence on the bioactivity of oligosaccharides from lactulose has encouraged us to study their physicochemical modifications during the processing of milk and apple juice.
A Aho, A Chandra, AL Buchsbaum, D Jacobs, F Afrati, F Bancilhon, F Bancilhon, F Bry, G Dong, G Dong, G Dong, GF Italiano, GG Hillerbrand, H Jakobsson, J. W. Lloyd, JM Nicolas, JW Lloyd, KR Apt, KR Apt, M Vardi, MH Emden, S Abiteboul, T Ibaraki, X Qian, Y Ioannidis   +24 more
core   +1 more source

New substrates for reliable enzymes: enzymatic modification of polymers [PDF]

, 2003
Recent studies clearly indicate that the modification of synthetic and natural polymers with enzymes is an environmentally friendly alternative to chemical methods using harsh conditions.
Gübitz, Georg M., Paulo, Artur Cavaco
core   +1 more source

The evaluation of in vitro cariogenicity of transgalactosylated oligosaccharide.

JOURNAL OF DENTAL HEALTH, 1990
In vitrocariogenicity of transgalactosylated oligosaccharide (TOS) was examined using oral streptococci. The results were as follows:1. Purified TOS (TOS-P) was not utilized for growth and production of lactic acid by oral streptococci. The utilization value of TOS-P was lower than that of neosugar-P.2. Association of TOS-P and TOS-50 with adherence in
Hisanori FUKUSHIMA, Yukari TOYOSHIMA, Jun-ichi INOUE, Hidenori MOROI, Yasuyuki TERAOKA, Takatoshi ONOE, Hirosuke SAGAWA   +6 more
openaire   +2 more sources

Evolved β-Galactosidases from Geobacillus stearothermophilus with Improved Transgalactosylation Yield for Galacto-Oligosaccharide Production [PDF]

Applied and Environmental Microbiology, 2009
ABSTRACT A mutagenesis approach was applied to the β-galactosidase BgaB from Geobacillus stearothermophilus KVE39 in order to improve its enzymatic transglycosylation of lactose into oligosaccharides.
Placier, G., Watzlawick, H., Rabiller, Claude, Mattes, R.   +3 more
openaire   +3 more sources