Results 21 to 30 of about 8,066 (202)

Structural analysis and functional evaluation of the disordered ß–hexosyltransferase region from Hamamotoa (Sporobolomyces) singularis

open access: yesFrontiers in Bioengineering and Biotechnology, 2023
Hamamotoa (Sporobolomyces) singularis codes for an industrially important membrane bound ß-hexosyltransferase (BHT), (BglA, UniprotKB: Q564N5) that has applications in the production of natural fibers such as galacto-oligosaccharides (GOS) and natural ...
Suzanne F. Dagher   +6 more
doaj   +1 more source

The P134Q Sucrose Phosphorylase: Subtle Changes in the Catalytic Properties Benefit the Production of 2-O-α-Glucosyl Glycerol. [PDF]

open access: yesBiotechnol Bioeng
Production of the cosmetic ingredient 2‐α‐d‐glucosyl glycerol (2GG) by the Pro134Gln variant of sucrose phosphorylase from Bifidobacterium adolescentis is reported. The authors show with detailed characterization and hybrid kinetic modeling that the enzyme variant can improve the 2GG productivity of the biocatalytic process due to its increased ...
Sigg A   +5 more
europepmc   +2 more sources

β-Glucosidase and β-Galactosidase-Mediated Transglycosylation of Steviol Glycosides Utilizing Industrial Byproducts

open access: yesFrontiers in Bioengineering and Biotechnology, 2021
Stevia rebaudiana Bertoni is a plant cultivated worldwide due to its use as a sweetener. The sweet taste of stevia is attributed to its numerous steviol glycosides, however, their use is still limited, due to their bitter aftertaste.
Anastasia Zerva   +4 more
doaj   +1 more source

Biochemical Characterization of a Novel Endo-1,3-β-Glucanase from the Scallop Chlamys farreri

open access: yesMarine Drugs, 2020
Endo-1,3-β-glucanases derived from marine mollusks have attracted much attention in recent years because of their unique transglycosylation activity.
Zhijian Li, Weizhi Liu, Qianqian Lyu
doaj   +1 more source

Replacement of the Catalytic Nucleophile Aspartyl Residue of Dextran Glucosidase by Cysteine Sulfinate Enhances Transglycosylation Activity [PDF]

open access: yes, 2013
Dextran glucosidase from Streptococcus mutans (SmDG) catalyzes the hydrolysis of an α-1,6-glucosidic linkage at the nonreducing end of isomaltooligosaccharides and dextran.
Kimura, Atsuo   +4 more
core   +1 more source

Thermodynamic reaction control of nucleoside phosphorolysis [PDF]

open access: yes, 2020
Nucleoside analogs represent a class of important drugs for cancer and antiviral treatments. Nucleoside phosphorylases (NPases) catalyze the phosphorolysis of nucleosides and are widely employed for the synthesis of pentose‐1‐phosphates and nucleoside ...
Giessmann, Robert T.   +4 more
core   +1 more source

Organosolv pretreatment of Sitka spruce wood: conversion of hemicelluloses to ethyl glycosides [PDF]

open access: yes, 2014
A range of organosolv pretreatments, using ethanol:water mixtures with dilute sulphuric acid, were applied to Sitka spruce sawdust with the aim of generating useful co-products as well as improving saccharification yield.
Bouxin, Florent P.   +2 more
core   +1 more source

Biotransformation of halogenated 2′-deoxyribosides by immobilized lactic acid bacteria [PDF]

open access: yes, 2012
An efficient and green bioprocess is herein reported to obtain halogenated nucleosides by transglycosylation using immobilized lactic acid bacteria (LAB).
Britos, Claudia Noelia   +5 more
core   +1 more source

Fluorescent turn-on probes for wash-free mRNA imaging via covalent site-specific enzymatic labeling. [PDF]

open access: yes, 2017
Investigating the many roles RNA plays in cellular regulation and function has increased demand for tools to explore RNA tracking and localization within cells.
Alexander, Seth C   +2 more
core   +2 more sources

Comparing Hydrolysis and Transglycosylation Reactions Catalyzed by Thermus thermophilus β-Glycosidase. A Combined MD and QM/MM Study

open access: yesFrontiers in Chemistry, 2019
The synthesis of oligosaccharides and other carbohydrate derivatives is of relevance for the advancement of glycosciences both at the fundamental and applied level. For many years, glycosyl hydrolases (GHs) have been explored to catalyze the synthesis of
Sonia Romero-Téllez   +7 more
doaj   +1 more source

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