Results 81 to 90 of about 120,068 (304)

Clinical Grade Treg: GMP Isolation, Improvement of Purity by CD127pos Depletion, Treg Expansion, and Treg Cryopreservation

PLoS ONE, 2008
Treg based immunotherapy is of great interest to facilitate tolerance in autoimmunity and transplantation. For clinical trials, it is essential to have a clinical grade Treg isolation protocol in accordance with Good Manufacturing Practice (GMP) guidelines.
Peters, J.H., Preijers, F.W.M.B., Woestenenk, R.M., Hilbrands, L.B., Koenen, H.J.P.M., Joosten, I.   +5 more
openaire   +5 more sources

Aptamer‐Engineered Liposomal Platform Enables in Situ cDC1 Vaccination to Potentiate Immunotherapy in Prostate Cancer

Advanced Science, EarlyView.
Prostate cancer is immunologically ‘cold’, with scarce, dysfunctional type 1 conventional dendritic cells (cDC1s) that limit T cell priming. We introduce an aptamer‐targeted liposomedelivering FMS‐like tyrosine kinase 3 ligand (Flt3L) and chlorin e6 (Ce6). Ultrasound induces antigen release and cDC1s recruitment, creating an in situ cDC1 vaccine.
Jiayi Wang, Xuan Wang, Xinfeng Dai, Linxin Tian, Wencheng Shen, Xueliang Liu, Wei Xue, Jiahua Pan, Yu Yang   +8 more
wiley   +1 more source

Engineering Approaches to Modify Immunomodulatory Functions of Mesenchymal Stromal Cells (MSCs): Tissue Regeneration and Clinical Application

Advanced Science, EarlyView.
Mesenchymal stromal cells (MSCs) show promise for treating immune‐related disorders through immunomodulation and tissue regeneration. This review gives a brief overview of current clinical approval of MSC therapies. It also discussed how bioengineering, including genetic modification, biomaterial delivery, extracellular vesicles, and iPSC‐derived MSCs,
Sichen Yang, Kejia Li, Ning Wang, Zhen Li, Zhiyong Zhang, Rocky S. Tuan, Yangzi Jiang   +6 more
wiley   +1 more source

Relative and absolute number of circulating Tregs.

, 2013
Within the CD3+CD4+ lymphogate, Tregs were defined as either being (A) CD25+FoxP3+ or being (B) CD25+CD127−. (C) The percentages of Tregs within the CD4+ T cell compartment. (D) Absolute number of circulating Tregs. The lines indicate the median values.
Joost Smolders (235367), Mariëlle Thewissen (235374), Evelyn Peelen (235371), Raymond Hupperts (235385), Paul Menheere (235380), Jan Damoiseaux (235391), Jan Willem Cohen Tervaert (235377)   +6 more
core   +1 more source

Integrative Multi‐Omics Analysis Reveals a Mitochondrial–Immune Axis Associated With Neoadjuvant Chemotherapy Response in High‐Grade Serous Ovarian Cancer

Advanced Science, EarlyView.
Integrative multi‐omics analysis delineates a mitochondrial–immune axis governing neoadjuvant chemotherapy response in high‐grade serous ovarian cancer. Immune‐active tumors exhibit enhanced B‐cell infiltration and favorable sensitivity, whereas metabolically rewired tumors display oxidative phosphorylation dependency and resistance.
Wei Jiang, Yi Wang, Xiaohang Lu, Qianlan Yao, Libing Xiang, Xu Cai, Xiaoyu Tu, Shaoxian Tang, Ying Xu, Yufan Cheng, Liming Shen, Huijuan Yang   +11 more
wiley   +1 more source

Comprehensive Explorations of CCL28 in Lung Adenocarcinoma Immunotherapy and Experimental Validation

Journal of Inflammation Research, 2023
Xiangyu Su,1,2 Guoqing Wang,3 Shiya Zheng,2 Chang Ge,4 Fei Kong,4 Cailian Wang1,2 1School of Medicine, Southeast University, Nanjing, 210009, People’s Republic of China; 2Department of Oncology, Zhongda Hospital, School of Medicine, Southeast University,
Su X, Wang G, Zheng S, Ge C, Kong F, Wang C   +5 more
doaj  

Phenotyping of expanded Tregs by flow cytometry.

, 2014
A. Representative examples of gating strategy used for CD25+FOXP3+ staining by flow-cytometry of ex vivo PBMC (upper panel) isolated from a HIV-1 controller and matched expanded Tregs (lower panel) at day 7 of expansion. B.
Melanie King (519982), Ildiko Toth (235721), Philip J. Goulder (519985), Douglas S. Kwon (517284), Alicja Piechocka-Trocha (151537), Ashley Rezai (519984), Marylyn M. Addo (379046), Andrew T. Chan (108141), Eshia S. Moodley (373514), Siddhartha M. Sharma (519983), Paul L. Klarenbeek (519981), Thumbi Ndung'u (104433), Mathieu Angin (519980)   +12 more
core   +1 more source

ROS Self‐Supply Nanoplatform Based on Fenton Catalyst for Chemodynamic and Immunotherapy: Reprogramming Cold Tumor Into Hot Tumor in Cancer Treatment

Advanced Science, EarlyView.
A multifunctional HA‐conjugated nanoplatform (HA‐PGMC) integrates CuO2, glucose oxidase, and mil‐100 to enable cascade catalytic ROS generation in tumor microenvironments. This self‐supplying ROS strategy induces immunogenic cell death, reprograms “cold” tumors into “hot” ones, and synergizes with PD‐L1 blockade, achieving potent chemodynamic ...
Man Lung Lee, Jack Chun Hin Chen, Wai Wing Cheng, Kwan Yee Lau, Hiu Yee Kwan, Ngar‐Yun Ellen Poon, Hung‐Wing Li   +6 more
wiley   +1 more source

Differential Protein Synthesis Rates in Tregs, conventional T cells, and ex-Tregs following LPS Stimulation 2332109

Introduction Protein synthesis is a metabolically demanding process that differs among T cell subsets based on their functional states and suppressive capacity.
Hayden Harach, Alexandra Staiculescu, Gabriela Ladino, Arit Ghosh   +3 more
core   +1 more source

Lactiplantibacillus Plantarum GUANKE Alleviates Allergic Asthma by Restricting Monocyte‐to‐DC Differentiation Via Microbiota‐Associated IAA

Advanced Science, EarlyView.
Lactiplantibacillus plantarum GUANKE remodels the indigenous gut microbiota and is associated with microbiota‐dependent restoration of circulating IAA. IAA treatment attenuates JAK3‐STAT3 and Jag1‐Notch4‐Hey1 signaling and reduces CX3CR1+Ly6C− monocytic precursor generation and pulmonary CD11b+ DC accumulation, together with reduced Th2 inflammation ...
Yujia He, Jielan Mi, Zhiping Zhao, Xiao Zhang, Zhihan Yang, Kun Yue, Yuanming Huang, Liqiong Song, Xiaoting Wen, Jianguo Xu, Zhihong Ren   +10 more
wiley   +1 more source