Results 11 to 20 of about 43,375 (307)
Dynamics of a Troponin Chimera Reproduce the Effects of Calcium on the Troponin Complex [PDF]
Biophysical Journal, 2012 In striated muscle, contraction is regulated in a Ca2+-dependent manner by the three subunits of the troponin complex. Troponin I (TnI) inhibits actomyosin ATPase in the absence of calcium; Ca2+ binding to troponin C (TnC) causes conformational changes that alter the interaction between TnI and TnC, removing the inhibition. These conformational changes
Olivier Julien +5 more
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NMR analysis of cardiac troponin C‐troponin I complexes: effects of phosphorylation [PDF]
FEBS Letters, 1999 Phosphorylation of the cardiac specific amino‐terminus of troponin I has been demonstrated to reduce the Ca2+ affinity of the cardiac troponin C regulatory site. Recombinant N‐terminal cardiac troponin I proteins, cardiac troponin I(33–80), cardiac troponin I(1–80), cardiac troponin I(1–80)DD and cardiac troponin I(1–80)pp, phosphorylated by protein ...
Natosha L. Finley +10 more
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Order-Disorder Transitions in the Cardiac Troponin Complex. [PDF]
J Mol Biol, 2016 The troponin complex is a molecular switch that ties shifting intracellular calcium concentration to association and dissociation of actin and myosin, effectively allowing excitation-contraction coupling in striated muscle. Although there is a long history of muscle biophysics and structural biology, many of the mechanistic details that enable troponin'
Metskas LA, Rhoades E.
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Journal of Biological Chemistry, 1974 Abstract By means of new or modified procedures troponin-tropomyosin complex was fractionated into three components plus tropomyosin, and then reconstituted in the absence of urea. Tropomyosin was separated from troponin by hydroxyapatite column chromatography, a method giving sharper separation than the previously used technique of isoelectric ...
Evan Eisenberg, W. Wayne Kielley
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Structure of the troponin complex. Implications of photocross-linking of troponin I to troponin C thiol mutants. [PDF]
Journal of Biological Chemistry, 1994 Ca2+ regulation of vertebrate-striated muscle contraction is initiated by conformational changes in the Ca(2+)-binding protein troponin C (TnC) and subsequent changes in the interaction of TnC with the inhibitory protein TnI. We have constructed mutants of rabbit skeletal muscle TnC in which natural Cys-98 was replaced by Leu, and a single Cys residue ...
Tetsuo Kobayashi +3 more
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Phosphorylation of the ‘37000 component’ of the troponin complex (troponin-T) [PDF]
Biochemical Journal, 1973 Most of the phosphorus present in the troponin complex, which on average contains 1 mol of P/80000g, is associated with the ‘37000 component’ (0.6mol of P/mol). The inhibitory protein and particularly the ‘37000 component’, but not the calcium-binding protein, were phosphorylated when incubated with phosphorylase b kinase and [γ-32P]ATP.
H. A. Cole, S. V. Perry
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Interaction of bepridil with the cardiac troponin C/troponin I complex [PDF]
FEBS Letters, 2001 We have investigated the binding of bepridil to calcium‐saturated cardiac troponin C in a cardiac troponin C/troponin I complex. Nuclear magnetic resonance spectroscopy and [15N,2H]cardiac troponin C permitted the mapping of bepridil‐induced amide proton chemical shifts. A single bepridil‐binding site in the regulatory domain was found with an affinity
Ekram Abusamhadneh +5 more
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Calcium-dependent Changes in the Flexibility of the Regulatory Domain of Troponin C in the Troponin Complex [PDF]
Journal of Biological Chemistry, 2005 With the recent advances in structure determination of the troponin complex, it becomes even more important to understand the dynamics of its components and how they are affected by the presence or absence of Ca(2+). We used NMR techniques to study the backbone dynamics of skeletal troponin C (TnC) in the complex.
Tharin M. A. Blumenschein +4 more
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Anti–Cardiac Troponin Autoantibodies Are Specific to the Conformational Epitopes Formed by Cardiac Troponin I and Troponin T in the Ternary Troponin Complex [PDF]
Clinical Chemistry, 2016 Abstract BACKGROUND Autoantibodies to cardiac troponins (TnAAbs) could negatively affect cardiac troponin I (cTnI) measurements by TnAAbs-sensitive immunoassays. We investigated the epitope specificity of TnAAbs and its influence on cTnI immunodetection in patients with acute myocardial infarction ...
Alexandra V Vylegzhanina +7 more
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Frontiers in Medicine, 2022 BackgroundChronic kidney disease has emerged as a significant independent risk factor for cardiovascular disease. Cardiovascular calcification is an active process involving a complex interaction of inducers and inhibitors.
Kamal M. Okasha +17 more
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