Ca2+-induced PRE-NMR changes in the troponin complex reveal the possessive nature of the cardiac isoform for its regulatory switch. [PDF]
PLoS ONE, 2014 The interaction between myosin and actin in cardiac muscle, modulated by the calcium (Ca2+) sensor Troponin complex (Tn), is a complex process which is yet to be fully resolved at the molecular level. Our understanding of how the binding of Ca2+ triggers
Nicole M Cordina +7 more
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Development of a specific troponin I detection system with enhanced immune sensitivity using a single monoclonal antibody [PDF]
Royal Society Open Science, 2020 Using an immunoassay in combination with surface plasmon fluorescence spectroscopy (SPFS), we report the rapid detection of troponin I, a valuable biomarker for diagnosis of myocardial infarction.
Anıl Bozdogan +5 more
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Computational Studies of Cardiac and Skeletal Troponin
Frontiers in Molecular Biosciences, 2019 Troponin is a key regulatory protein in muscle contraction, consisting of three subunits troponin C (TnC), troponin I (TnI), and troponin T (TnT). Calcium association to TnC initiates contraction by causing a series of dynamic and conformational changes ...
Jacob D. Bowman, Steffen Lindert
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Journal of Health Sciences, 2011 Introduction: The aim of this study was determination of troponin I at serum using Architect (Abbott) and AxSYM System (Abbott). Troponin is regulatory subunit of the troponin complex associate with actin filament within muscle cells and it is a marker ...
Nafija Serdarević
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Distance distributions and anisotropy decays of troponin C and its complex with troponin I [PDF]
Biochemistry, 1991 We used frequency domain measurements of fluorescence resonance energy transfer to recover the distribution of distances between Met 25 and Cys 98 in rabbit skeletal troponin C. These residues were labeled with dansylaziridine as energy donor and 5-(iodoacetamido)eosin as acceptor and are located on the N- and C-terminal lobes of the two-domain protein,
Wiesław Wiczk +6 more
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A Case Series of Disproportionate Elevations of Cardiac Troponin and Macrotroponin in Fabry Disease [PDF]
Journal of Inborn Errors of Metabolism and ScreeningFabry disease is a rare X-linked lysosomal storage disorder that causes progressive cellular accumulation of glycosphingolipids, leading to various end-organ manifestations such as chronic kidney disease and cardiomyopathy.
Yvonne Moussa +5 more
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Effects of troponin T cardiomyopathy mutations on the calcium sensitivity of the regulated thin filament and the actomyosin cross-bridge kinetics of human β-cardiac myosin. [PDF]
PLoS ONE, 2013 Hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM) lead to significant cardiovascular morbidity and mortality worldwide. Mutations in the genes encoding the sarcomere, the force-generating unit in the cardiomyocyte, cause familial forms ...
Ruth F Sommese +5 more
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The calcium‐induced switch in the troponin complex probed by fluorescent mutants of troponin I [PDF]
European Journal of Biochemistry, 2003 The Ca2+‐induced transition in the troponin complex (Tn) regulates vertebrate striated muscle contraction. Tn was reconstituted with recombinant forms of troponin I (TnI) containing a single intrinsic 5‐hydroxytryptophan (5HW). Fluorescence analysis of these mutants of TnI demonstrate that the regions in TnI that respond to Ca2+ binding to the ...
Deodoro Camargo Silva Gonçalves de Oliveira +1 more
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Bovine cardiac troponin subunits: binary complexes and reconstitution of whole troponin
FEBS Letters, 1982 The reconstitution of bovine cardiac troponin from its subunits has been investigated using hydrodynamic techniques. Gel filtration (Sephacryl S‐300) and sedimentation velocity experiments indicate that troponin‐C and troponin‐I from a stable binary complex (1:1 mole ratio) with an apparent Stokes' radius of 36 Å (frictional ratio = 1.6).
David M. Byers, Cyril M. Kay
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Calcium induced regulation of skeletal troponin--computational insights from molecular dynamics simulations. [PDF]
PLoS ONE, 2013 The interaction between calcium and the regulatory site(s) of striated muscle regulatory protein troponin switches on and off muscle contraction. In skeletal troponin binding of calcium to sites I and II of the TnC subunit results in a set of structural ...
Georgi Z Genchev +2 more
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