Results 181 to 190 of about 50,586 (204)

Characterisation of pentamidine-resistant Trypanosoma brucei brucei

Molecular and Biochemical Parasitology, 1995
Following selection in vitro by exposure to increasing concentrations of the aromatic diamidine pentamidine, a Trypanosoma brucei brucei clone has been characterised in vivo and in vitro. The resistant clone, designated T.b. brucei S427/118/PR32.6, was found to be less virulent than the parental clone T.b.
Berger, Bradley J., Carter, Nicola S., Fairlamb, Alan H.   +2 more
openaire   +3 more sources

Differential Effects of Trypanosoma brucei gambiense and Trypanosoma brucei brucei on Rat Macrophages

Journal of Parasitology, 2011
Mammalian immune responses to Trypanosoma brucei infection are important to control of the disease. In rats infected with T. brucei gambiense (Wellcome strain; WS) or T. brucei brucei (interleukin-tat 1.4 strain [ILS]), a marked increase in the number of macrophages in the spleen can be observed.
Kazuhiko, Nishimura, Haruna, Nakaya, Hiroshi, Nakagawa, Saburo, Matsuo, Yoshihiro, Ohnishi, Shinji, Yamasaki   +5 more
openaire   +2 more sources

Biosynthesis of Trypanothione in Trypanosoma brucei brucei

Biological Chemistry, 2003
Trypanothione [T(SH)2], the major redox mediator in pathogenic trypanosomatids, is synthetized stepwise by two distinct enzymes in Crithidia fasciculata, while in Trypanosoma cruzi a single enzyme catalyzes both steps. A full-length reading frame presumed to encode trypanothione synthetase (TryS) was obtained by PCR using DNA of T.
Marcelo, Comini, Ulrich, Menge, Leopold, Flohé   +2 more
openaire   +2 more sources

Transketolase in Trypanosoma brucei

Molecular and Biochemical Parasitology, 2011
A single copy gene, encoding a protein highly similar to transketolase from other systems, was identified in the Trypanosoma brucei genome. The gene was expressed in E. coli and the purified protein demonstrated transketolase activity with K(m) values of 0.2mM and 0.8mM respectively for xylulose 5-phosphate and ribose 5-phosphate.
Stoffel, Sabine A., Alibu, Vincent P., Hubert, Jane, Ebikeme, Charles, Portais, Jean-Charles, Bringaud, Frédéric, Schweingruber, M. Ernst, Barrett, Michael P.   +7 more
openaire   +3 more sources

Kinetics of Methionine Transport and Metabolism by Trypanosoma brucei brucei and Trypanosoma brucei rhodesiense

Archives of Biochemistry and Biophysics, 2000
Methionine is an essential amino acid for both prokaryotic and eukaryotic organisms; however, little is known concerning its utilization in African trypanosomes, protozoa of the Trypanosoma brucei group. This study explored the Michaelis-Menten kinetic constants for transport and pool formation as well as metabolic utilization of methionine by two ...
B, Goldberg, D, Rattendi, D, Lloyd, N, Yarlett, C J, Bacchi   +4 more
openaire   +2 more sources

Porcine cerebralTrypanosoma brucei brucei trypanosomiasis

Tropical Animal Health and Production, 1991
This paper describes the investigation of a disease outbreak among 10 adult pigs in Nsukka, Anambra State, Nigeria. Prior to the investigation one sow died of the disease. Trypanosomes were later detected in the blood of two of the nine pigs subsequently investigated.
D N, Onah, M, Uzoukwu
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Characterization of spermidine synthase from Trypanosoma brucei brucei

Molecular and Biochemical Parasitology, 1984
Spermidine synthase from Trypanosoma brucei brucei was characterized and found to be similar to spermidine synthase from other sources. The Km for putrescine was found to be 0.2 mM and the Km for decarboxylated S-adenosylmethionine 0.1 microM. The approximate molecular weight of the enzyme was 74 000 as determined by a combination of molecular sieve ...
A J, Bitonti, S E, Kelly, P P, McCann
openaire   +2 more sources

The Ubiquitin-Ligase System in Trypanosoma Brucei Brucei

The American Journal of Tropical Medicine and Hygiene, 1993
The question of whether the African trypanosome Trypanosoma brucei brucei utilizes a ubiquitin-ligase system to conjugate the 8,500-dalton protein ubiquitin to other proteins has not been investigated. Using 125I-labeled ubiquitin and gel electrophoresis (sodium dodecyl sulfate and acetic acid, urea, Triton X-100), we looked for the incorporation of ...
D J, Lowrie, B F, Giffin, R M, Ventullo
openaire   +2 more sources

Rodent-free cyclical transmission of Trypanosoma brucei brucei

Molecular and Biochemical Parasitology, 2017
We provide a simple protocol enabling cyclical transmission of Trypanosoma brucei brucei to be performed without the need for mammals. These procedures have two advantages: they are in line with 3R principles of animal use - replace, refine, reduce - and may enable more laboratories to study the complete life cycle.
Arunasalam, Naguleswaran, Isabel, Roditi
openaire   +2 more sources

Characterization of different proteolytic activities in Trypanosoma brucei brucei

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1992
The variant surface glycoprotein of African trypanosomes is released after overnight incubation of parasites at 4 degrees C in pH 5.5 phosphate glucose buffer and may be purified by Concanavalin A Sepharose affinity chromatography. The addition of proteinase inhibitors during the parasite incubation is necessary to prevent the proteolysis of the ...
G, Huet, C, Richet, D, Demeyer, H, Bisiau, B, Soudan, D, Tetaert, K K, Han, P, Degand   +7 more
openaire   +2 more sources