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Trypanosoma cruzi: polypeptide markers of epimastigotes and trypomastigotes

Molecular and Biochemical Parasitology, 1986
We compared the major polypeptides of epimastigotes and trypomastigotes of T. cruzi, by submitting total parasite lysates to electrophoresis in polyacrylamide gels (SDS-PAGE), protein staining with Coomassie brilliant blue, laser densitometry, or immunoblotting with sera derived from infected individuals (Chagas' disease).
Rafael Rangel-Aldao   +2 more
exaly   +3 more sources

Steroidogenic capacity of Trypanosoma cruzi trypomastigotes

The Journal of Steroid Biochemistry and Molecular Biology, 2008
American Trypanosomiasis is caused by the hemoflagellate Trypanosoma cruzi (T. cruzi) and affects millions of persons causing variable degrees of digestive and heart disturbances. As far as we concerned, T. cruzi capacity to synthesize steroid hormones has not been investigated.
P, Vacchina   +4 more
openaire   +2 more sources

Comparison of infectivity of "Trypanosoma cruzi" blood stream trypomastigotes and metacyclic trypomastigotes from "Rhodnius prolixus"

Acta tropica, 1977
Four strains of Trypanosoma cruzi (Y, BG, M and Peru) retain their ability to infect Rhodnius prolixus and to produce virulent infections in mice for from 12 to 39 years. About 60 or more metacyclic trypomastigotes were consistently lethal to mice. The mean number of metacyclics per bug ranged from 1.2 to 17.3 x 10(3).
R A, Neal, N, McHardy
openaire   +2 more sources

Effect of Gossypol on Trypomastigotes and Amastigotes of Trypanosoma cruzi

The Journal of Protozoology, 1990
ABSTRACTBloodstream Trypanosoma cruzi trypomastigotes isolated from infected mice undergo reduction of motility and structural damages after 5 to 45 min exposure to gossypol at concentrations ranging from 5 to 50 μM. When 1% serum albumin is added to the incubation medium, no alterations of parasites are observed, even with 100 μM gossypol ...
L E, Rovai   +3 more
openaire   +2 more sources

Trypanosoma cruzi: metabolic labeling of trypomastigote sialoglycolipids

Experientia, 1985
Trypomastigote forms (infective) of Trypanosoma cruzi incorporate (3H)-palmitic acid and D-(3H)-galactose into glycolipids. Palmitic acid-labeled acidic glycolipids were partially hydrolyzed with neuraminidase. The labeling of these compounds when the intact cell surface was labeled with galactose oxidase plus NaB3H4 indicates the membrane location of ...
A S, Couto   +3 more
openaire   +2 more sources

Characterization of inositolphospholipids in Trypanosoma cruzi trypomastigote forms

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1996
In vivo labeling experiments with [3H]palmitic acid, [3H]inositol, and [3H]glucose allowed the identification of two main classes of inositolphospholipids (IPLs) from the trypomastigote stage of Trypanosoma cruzi. Purification of these compounds was achieved by ion-exchange chromatography, high performance liquid chromatography and thin layer ...
M L, Uhrig   +3 more
openaire   +2 more sources

Effects on Trypanosoma brucei Differentiating Bloodstream Trypomastigotes and Established Procyclic Trypomastigotes When Grown in the Presence of Respiratory Inhibitors

The Journal of Parasitology, 1986
Il semble au bout de cette etude que ni la differenciation des trypomastigotes des formes sanguines ni les trypomastigotes procycliques ne sont capables de survivre par un mouvement rapide a une voie respiratoire alternative lorsque l'oxydase primaire est ...
R B, Le Febvre, G C, Hill
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Localization of hydrolases in cultured procyclic trypomastigotes of Trypanosoma brucei

Molecular and Biochemical Parasitology, 1981
Procyclic culture forms of Trypanosoma brucei were examined for enzymes that could serve as markers for the plasma membrane, flagellar pocket, lysosomes, endoplasmic reticulum and Golgi apparatus in order to study the subcellular organization of the digestive system of the parasite.
F R, Opperdoes, R F, Steiger
openaire   +2 more sources

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