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Effects of Human Defensin-α1 on Trypanosoma cruzi Trypomastigotes in Vitro [PDF]
Bulletin of Experimental Biology and Medicine, 2010 Human defensin-α(1)is a biologically active peptide exhibiting a dose-dependent trypanocidal effect in vitro against trypomastigotes and amastigotes of Trypanosoma cruzi line Tulahuen. This effect is determined by fragmentation of parasite DNA reducing the capacity of passaged T. cruzi to invade HeLa cells.
Fernando Villalta
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Isolation of Bloodstream Trypomastigotes of Trypanosoma cruzi by a Gradient of Metrizamide
The Journal of Parasitology, 1980Several methods have been used to isolate bloodstream forms (trypomastigotes) of trypanosomes from other blood components. Four of them have been widely applied: (1) defibrination of the blood by incubation in a medium containing calcium chloride and shaking with glass beads followed by passage of the parasites through a sintered glass funnel (Fulton ...
M A, Loures, P F, Pimenta, W, de Souza
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Fibronectin receptors on Trypanosoma cruzi trypomastigotes and their biological function
Nature, 1984Successful invasion of mammalian cells by pathogenic parasites is generally considered, from circumstantial evidence, to be a consequence of specific mechanisms of recognition of cell surface components--this has stimulated investigations of the biochemical characterization of such molecules. Several studied of trypanosomiasis have examined the ability
M A, Ouaissi +3 more
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New Methods for the Isolation of Bloodstream Trypomastigotes of Trypanosoma cruzi
The Journal of Parasitology, 1981fact, Cressey and Lachner (1970, loc. cit.) observed that remoras' stomachs usually did not contain copepod species which normally infested sharks' fins. Furthermore, clustering might also assist hydrodynamically in copepod attachment. As water passes over the cluster, the posterior of each individual should transfer a downward force to the underlying ...
K D, Hungerer +2 more
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In vitro Production of Metacyclic Trypomastigotes of Trypanosoma cruzi
The Journal of Parasitology, 1980Bloodstream-forms of Trypanosoma cruzi ingested by reduviid bugs multiply and differentiate through a series of developmental stages into infective, metacyclic, trypomastigote forms. The intestinal tracts of these bugs provide a suitable physical and chemical environment for the complex developmental cycle of this stercorarian parasite.
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Transactions of the Royal Society of Tropical Medicine and Hygiene, 1979
Groups of CD-1 mice were immunized with vaccines prepared from freeze-thawed or ultra-sonicated epimastigotes, blood trypomastigotes, or "plasma antigen", of Trypanosoma cruzi strains Y, M1 and Tulahuen. The mice were challenged by the injection of blood stream trypomastigotes obtained from mice, or of metacyclic trypomastigotes harvested from the ...
N, McHardy, R A, Neal
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Groups of CD-1 mice were immunized with vaccines prepared from freeze-thawed or ultra-sonicated epimastigotes, blood trypomastigotes, or "plasma antigen", of Trypanosoma cruzi strains Y, M1 and Tulahuen. The mice were challenged by the injection of blood stream trypomastigotes obtained from mice, or of metacyclic trypomastigotes harvested from the ...
N, McHardy, R A, Neal
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Comprehensive glycoprofiling of the epimastigote and trypomastigote stages of Trypanosoma cruzi
Journal of Proteomics, 2017Trypanosoma cruzi, the protozoan that causes Chagas disease, has a complex life cycle involving insect and mammalian hosts and distinct developmental stages. During T. cruzi developmental stages, glycoproteins play important role in the host-parasite interaction, such as cellular recognition, host cell invasion and adhesion, and immune evasion. In this
Maria Julia Manso Alves +7 more
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Temperature acclimation of Trypanosoma cruzi epimastigote and metacyclic trypomastigote lipids
Molecular and Biochemical Parasitology, 1997This study examines the changes in cellular lipids that take place when Trypanosoma cruzi epimastigotes and metacyclic trypomastigotes are transferred from 28 to 37 degrees C. We found a rise in the sterol to phospholipid ratio, as well as in the triacylglycerol and steryl ester cellular content in T. cruzi epimastigotes.
Florin-Christensen, M. +6 more
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Glycophosphatidylinositol-anchored proteins in metacyclic trypomastigotes of Trypanosoma cruzi
Molecular and Biochemical Parasitology, 1988We searched for the presence of glycophosphatidylinositol (GPI)-anchored proteins in epimastigotes and metacyclic trypomastigotes of Trypanosoma cruzi, by treatment of parasite lysates with the GPI-specific phospholipase C of Trypanosoma brucei. Upon treatment, several proteins (70-90 kDa) in metacyclics, but none in epimastigotes, reacted with ...
Schenkman, S. +2 more
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Trypanosoma rhodesiense: Mitochondrial proteins of bloodstream and procyclic trypomastigotes
Experimental Parasitology, 1986One- and two-dimensional gel electrophoresis of the solubilized mitochondrial proteins of bloodstream and procyclic trypomastigote Trypanosoma brucei rhodesiense and radiolabeling of proteins in the presence of cycloheximide were used to identify proteins synthesized in the trypanosome mitochondrion.
R B, Le Febvre, G C, Hill
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