Topological patterns in two-dimensional gel electrophoresis of DNA knots [PDF]
Gel electrophoresis is a powerful experimental method to probe the topology of DNA and other biopolymers. While there is a large body of experimental work which allows us to accurately separate different topoisomers of a molecule, a full theoretical ...
Marenduzzo , Davide +2 more
core +4 more sources
Development of high-performance two-dimensional gel electrophoresis for human hair shaft proteome. [PDF]
The primary components of human hair shaft-keratin and keratin-associated proteins (KAPs), together with their cross-linked networks-are the underlying reason for its rigid structure.
Sing Ying Wong +2 more
doaj +2 more sources
Two-dimensional gel electrophoresis data for proteomic profiling of Sporothrix yeast cells [PDF]
Sporotrichosis is a chronic infection of the skin and subcutaneous tissues of human and other mammals caused by a complex of cryptic dimorphic fungi in the plant-associated order Ophiostomatales.
Anderson Messias Rodrigues +5 more
doaj +2 more sources
Two-dimensional gel electrophoresis in bacterial proteomics [PDF]
Two-dimensional gel electrophoresis (2-DE) is a gel-based technique widely used for analyzing the protein composition of biological samples. It is capable of resolving complex mixtures containing more than a thousand protein components into individual protein spots through the coupling of two orthogonal biophysical separation techniques: isoelectric ...
Watt, RM +3 more
openaire +5 more sources
Ocular Proteomics with Emphasis on Two-Dimensional Gel Electrophoresis and Mass Spectrometry [PDF]
The intention of this review is to provide an overview of current methodologies employed in the rapidly developing field of ocular proteomics with emphasis on sample preparation, two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass ...
Mandal Nakul +4 more
doaj +2 more sources
Two-dimensional gel electrophoresis data in support of leaf comparative proteomics of two citrus species differing in boron-tolerance [PDF]
Here, we provide the data from a comparative proteomics approach used to investigate the response of boron (B)-tolerant ‘Xuegan’ (Citrus sinensis) and B-intolerant ‘Sour pummelo’ (Citrus grandis) leaves to B-toxicity.
Wen Sang +5 more
doaj +2 more sources
Comparative Two-Dimensional Fluorescence Gel Electrophoresis [PDF]
Abstract Comparative two-dimensional gel electrophoresis (CoFGE) is a special version of two-dimensional polyacrylamide GE (2D-PAGE) and related to difference GE (2D-DIGE). It provides reproducibility and standardisation for 2D-PAGE by introducing a reference to the experiment.
Simone König, Doreen Ackermann
openaire +4 more sources
A robust high-sensitivity algorithm for automated detection of proteins in two-dimensional electrophoresis gels [PDF]
The automated interpretation of two-dimensional gel electrophoresis images used in protein separation and analysis presents a formidable problem in the detection and characterization of ill-defined spatial objects.
Harrington, Michael G. +1 more
core +1 more source
En este estudio demostramos el potencial de la electroforesis en dos dimensiones (2DE) como herramienta para la caracterización del proteoma de Leishmania (expresión proteica complementaria del genoma).
Rafael Góngora +5 more
doaj +1 more source
Comparative two-dimensional gel analysis and microsequencing identifies gelsolin as one of the most prominent downregulated markers of transformed human fibroblast and epithelial cells [PDF]
A systematic comparison of the protein synthesis patterns of cultured normal and transformed human fibroblasts and epithelial cells, using two-dimensional gel protein analysis combined with computerized imaging and data acquisition, identified a 90-kD ...
Bauw, Guy +4 more
core +1 more source

