Dissociation and reassembly of Escherichia coli type 1 pili [PDF]
Escherichia coli type 1 pili, which mediate the mannose-sensitive adherence of the bacterium to eucaryotic cells, are comprised of very stable arrays of pilin protein subunits (molecular weight, approximately 17,000). Previous methods for the dissociation of pili caused their irreversible denaturation. We have found that incubation of pili in saturated
Y, Eshdat, F J, Silverblatt, N, Sharon
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Characterization of type 1 pili of Salmonella typhimurium LT2 [PDF]
Type 1 pili from Salmonella typhimurium LT2 were purified and characterized. The pilus filaments were 6 nm in diameter and over 1 microns long. Estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the molecular weight of the pilin was 21,000. The isoelectric point of the filament was 4.1.
T K, Korhonen +3 more
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Molecular mechanism of bacterial type 1 and P pili assembly [PDF]
The formation of adhesive surface structures called pili or fimbriae (‘bacterial hair’) is an important contributor towards bacterial pathogenicity and persistence. To fight often chronic or recurrent bacterial infections such as urinary tract infections, it is necessary to understand the molecular mechanism of the nanomachines assembling such pili ...
Andreas, Busch +2 more
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Streptococcus sanguinis is a common cause of infective endocarditis (IE). Efforts by research groups are aimed at identifying and characterizing virulence factors that contribute to the ability of this organism to cause IE.
Anthony M. Martini +3 more
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A Small Non-Coding RNA Modulates Expression of Pilus-1 Type in Streptococcus pneumoniae
Streptococcus pneumoniae is a major cause of morbidity and mortality worldwide, and about 30% of the pneumococcal clinical isolates show type I pili-like structures.
Paloma Acebo +6 more
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Association of type 1 pili with the ability of livers to clear Salmonella typhimurium [PDF]
The role of type 1 pili in the adherence of Salmonella typhimurium strain SR-11 to hepatic sinusoidal cells was investigated. An average of 66.7% of piliated organisms was cleared by perfused livers on a single pass. Mannose and alpha-methyl-D-mannoside inhibited such trapping in a dose-dependent manner. Preincubation of the bacteria, but not the liver,
R D, Leunk, R J, Moon
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A host receptor enables type 1 pilus-mediated pathogenesis of Escherichia coli pyelonephritis.
Type 1 pili have long been considered the major virulence factor enabling colonization of the urinary bladder by uropathogenic Escherichia coli (UPEC). The molecular pathogenesis of pyelonephritis is less well characterized, due to previous limitations ...
Lisa K McLellan +12 more
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Frequency among Enterobacteriaceae of the DNA sequences encoding type 1 pili [PDF]
Type 1 pili, characterized by mannose-inhibitable agglutination of fowl or guinea pig erythrocytes, have been found throughout the family Enterobacteriaceae. A radiolabeled probe was prepared from a restriction endonuclease-digested fragment of the Escherichia coli pil operon and used to detect homologous DNA sequences in 236 bacteria representing 11 ...
K, Buchanan +3 more
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Distribution of type 1 and P pili on uropathogenic Escherichia coli O6 [PDF]
The distribution of type 1 and P pili on individual cells of an O6 uropathogenic Escherichia coli strain, 6260, was determined immunologically with pilus-specific monoclonal antibodies by indirect immunofluorescence and immunogold electron microscopy.
R M, Gander, V L, Thomas
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Human Neutrophil’S Chemotaxis and Intracellular Killing in Response to Type 1 Piliated Uropathogenic Escherichia Coli [PDF]
Introduction: Uropathogenic Escherichia coli (UPEC), the commonest cause of urinary tract infections, bind to target cells and phagocytes via several distinct pairs of adhesins and receptors.
N Nooritalab, SH Najar Peerayeh
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