Antimicrobial activity of clofazimine is not dependent on mycobacterial C-type phospholipases [PDF]
Journal of Antimicrobial Chemotherapy, 2004 We have used a phospholipase C (PLC)-deletion mutant (plcABC) of the H37Rv strain of Mycobacterium tuberculosis (MTB), as well as a plcA-insertion mutant of Mycobacterium smegmatis, to investigate the possible involvement of PLCs in clofazimine-mediated inhibition of mycobacterial K(+) transport and growth. Inactivation of the PLCs of MTB and insertion
M C Bopape
core +4 more sources
Toxins, 2016 Venoms of most Russian viper species are poorly characterized. Here, by quantitative chromato-mass-spectrometry, we analyzed protein and peptide compositions of venoms from four Vipera species (V. kaznakovi, V. renardi, V. orlovi and V.
Sergey I. Kovalchuk +4 more
doaj +2 more sources
Selective Blockade of P/Q-Type Calcium Channels by the Metabotropic Glutamate Receptor Type 7 Involves a Phospholipase C Pathway in Neurons [PDF]
The Journal of Neuroscience, 2000 Although presynaptic localization of mGluR7 is well established, the mechanism by which the receptor may control Ca2+channels in neurons is still unknown. We show here that cultured cerebellar granule cells express native metabotropic glutamate receptor type 7 (mGluR7) in neuritic processes, whereas transfected mGluR7 was also expressed in cell bodies.
Julie Perroy +5 more
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Purification of two distinct types of phosphoinositide-specific phospholipase C from rat liver. Enzymological and structural studies [PDF]
Biochemical Journal, 1988 Two kinds of phosphoinositide-specific phospholipase C (PLC) were purified from rat liver by acid precipitation and several steps of column chromatography. About 50% of the activity could be precipitated when the pH of the liver homogenate was lowered to pH 4.7. The redissolved precipitate yielded two peaks, PLC I and PLC II, in an Affi-gel Blue column,
Osamu Nakanishi +5 more
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Cloning of the Human Phospholipase C-γ1 Promoter and Identification of a DR6-type Vitamin D-responsive Element [PDF]
Journal of Biological Chemistry, 1997 The 5'-flanking region of the human phospholipase C-gamma1 gene was isolated from a human P1 genomic DNA library. The S1-nuclease mapping and primer extension analysis revealed that there is a single transcriptional start site located at 135 bases upstream from the translation start codon in the human phospholipase C-gamma1 gene.
Zhongjian Xie, Daniel D. Bikle
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A Novel C. elegans Memory Type Mediated by an Insulin/Phospholipase C Pathway [PDF]
, 2023 Abstract Memories are often categorized into types, reflecting their behavioral, anatomical and molecular diversity: these classifications both aid understanding of the differences among varieties of memory and help delineate the unifying cross-species principles underlying them. In the nematode worm
Daniel M. Merritt +6 more
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G Protein β Subunit Types Differentially Interact with a Muscarinic Receptor but Not Adenylyl Cyclase Type II or Phospholipase C-β2/3 [PDF]
Journal of Biological Chemistry, 2001 In comparison with the alpha subunit of G proteins, the role of the beta subunit in signaling is less well understood. During the regulation of effectors by the betagamma complex, it is known that the beta subunit contacts effectors directly, whereas the role of the beta subunit is undefined in receptor-G protein interaction.
Yongmin Hou +4 more
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Phylogenetic analysis of phospholipase C genes from Clostridium perfringens types A to E and Clostridium novyi [PDF]
Journal of Bacteriology, 1995 The phylogenetic interrelationships between strains of 5 toxin types (A to E) of Clostridium perfringens were examined by analysis of differences in the nucleotide sequences of phospholipase C genes (plc genes) among 10 strains, including 3 strains for which the plc gene sequences have been previously reported.
Ken‐Ichiro Tsutsui +7 more
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Mechanisms of Phospholipase C Activation by the Vasoactive Intestinal Polypeptide/Pituitary Adenylate Cyclase-Activating Polypeptide Type 2 Receptor [PDF]
Endocrinology, 2001 The vasoactive intestinal polypeptide/pituitary adenylate cyclase-activating polypeptide type 2 (VPAC(2)) receptor was shown to induce both [(3)H]inositol phosphate ([(3)H]InsP)and cAMP production in transfected COS7 cells and in GH(3) cells where it is natively expressed.
Chris Mackenzie
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Tools and mechanisms of vacuolar escape leading to host egress in Legionella pneumophila infection: Emphasis on bacterial phospholipases [PDF]
, 2023 The phenomenon of host cell escape exhibited by intracellular pathogens is a remarkably versatile occurrence, capable of unfolding through lytic or non-lytic pathways. Among these pathogens, the bacterium Legionella pneumophila stands out, having adopted
Aurass, Philipp +3 more
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