Results 71 to 80 of about 26,046,637 (294)

Biogenesis, Regulation, and Targeting of the Type III Secretion System [PDF]

Structure, 2011
The type III secretion system (T3SS) is employed by a number of Gram-negative bacterial pathogens to inject toxins into eukaryotic cells. The biogenesis of this complex machinery requires the regulated interaction between over 20 cytosolic, periplasmic, and membrane-imbedded proteins, many of which undergo processes such as polymerization, partner ...
Izoré, Thierry, Job, Viviana, Dessen, Andréa   +2 more
openaire   +2 more sources

Decoding the dynamic extracellular matrix in cancer—3D models and bioscaffolds rewire the rules of tumor progression

FEBS Letters, EarlyView.
Cancer progression is regulated by the dynamic matrix code of the tumor microenvironment, which influences cellular behavior and disease development. Importantly, matrix remodeling in three‐dimensional cancer models more accurately reflects in vivo conditions compared to conventional two‐dimensional systems.
Sylvia Mangani, Konstantinos Spanopoulos, Zoi Piperigkou, Nikos K. Karamanos   +3 more
wiley   +1 more source

The central metabolism regulator EIIAGlc switches Salmonella from growth arrest to acute virulence through activation of virulence factor secretion [PDF]

, 2014
The ability of Salmonella to cause disease depends on metabolic activities and virulence factors. Here, we show that a key metabolic protein, EIIAGlc, is absolutely essential for acute infection, but not for Salmonella survival, in a mouse typhoid fever ...
Timo Glatter, Dirk Bumann,, Glatter, Timo, Glatter, T. ; https://orcid.org/, Mazé, Alain, Bumann, Dirk, Timo Glatter,, Dirk Bumann, Alain Mazé, Maze, A., Alain Mazé,, Bumann, D.   +11 more
core   +1 more source

Cryo-EM of the injectisome and type III secretion systems

Current Opinion in Structural Biology, 2022
Double-membrane-spanning protein complexes, such as the T3SS, had long presented an intractable challenge for structural biology. As a consequence, until a few years ago, our molecular understanding of this fascinating complex was limited to composite models, consisting of structures of isolated domains, positioned within the overall complex.
Julien R.C. Bergeron, Thomas C. Marlovits   +1 more
openaire   +4 more sources

Discerning protein pools by selective staining with self‐labeling tags

FEBS Letters, EarlyView.
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley   +1 more source

Structural analysis of Salmonella enterica effector protein SopD [PDF]

, 2004
Salmonella outer protein D (SopD) is a type III secreted virulence effector protein from Salmonella enterica. Full-length SopD and SopD lacking 16 amino acids at the N-terminus (SopDDeltaN) have been expressed as fusions with GST in Escherichia coli ...
Williams, C, Gill, A C, Galyov, E E, van den Elsen, J M H, Upadhyay, A, Bagby, S, Wood, M W, Philippe, D L   +7 more
core   +1 more source

Functional regulation and cross-talk of type III and type VI secretion systems in Salmonella

Virulence
In Salmonella, the type III and type VI secretion systems (T3SS and T6SS) play critical roles in pathogenicity. The bacterium harbors two distinct T3SSs: T3SS-1, encoded by SPI-1, promotes host cell invasion, whereas T3SS-2 (located within SPI-2 ...
Xingyi Li, Ying Ding, Yu Sun, Yiting Gu, Wanli Feng, Yanyan Jia, Huimin Chen, Songbiao Chen   +7 more
doaj   +1 more source

Five Plant Natural Products Are Potential Type III Secretion System Inhibitors to Effectively Control Soft-Rot Disease Caused by Dickeya

Frontiers in Microbiology, 2022
Dickeya zeae, a plant soft-rot pathogen, possesses a type III secretion system (T3SS) as one of the major virulence factors, infecting a wide variety of monocotyledonous and dicotyledonous plants and causing serious losses to the production of economic ...
Anqun Hu, Ming Hu, Shanshan Chen, Yang Xue, Xu Tan, Jianuan Zhou   +5 more
doaj   +1 more source

Type III secretion systems: the bacterial flagellum and the injectisome [PDF]

Philosophical Transactions of the Royal Society B: Biological Sciences, 2015
The flagellum and the injectisome are two of the most complex and fascinating bacterial nanomachines. At their core, they share a type III secretion system (T3SS), a transmembrane export complex that forms the extracellular appendages, the flagellar filament and the injectisome needle. Recent advances, combining structural biology,
Andreas Diepold, Judith P. Armitage
openaire   +3 more sources

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source