Results 11 to 20 of about 851 (145)

Light‐Activatable Ubiquitin for Studying Linkage‐Specific Ubiquitin Chain Formation Kinetics [PDF]

Advanced Science
Ubiquitination is a dynamic post‐translational modification governing protein abundance, function, and localization in eukaryotes. The Ubiquitin protein is conjugated to lysine residues of target proteins, but can also repeatedly be ubiquitinated itself,
Sudakshina Banerjee, Zeyneb Vildan Cakil, Kai Gallant, Johannes van den Boom, Shubhendu Palei, Hemmo Meyer, Malte Gersch, Daniel Summerer   +7 more
doaj   +5 more sources

Time-resolved in vivo ubiquitinome profiling by DIA-MS reveals USP7 targets on a proteome-wide scale [PDF]

Nature Communications, 2021
Combining improved sample preparation, data-independent acquisition mass spectrometry and deep learning, the authors develop a workflow for more robust and precise quantitative ubiquitinome profiling.
Martin Steger, Vadim Demichev, Mattias Backman, Uli Ohmayer, Phillip Ihmor, Stefan Müller, Markus Ralser, Henrik Daub   +7 more
doaj   +6 more sources

A targeted multi-proteomics approach generates a blueprint of the ciliary ubiquitinome [PDF]

Frontiers in Cell and Developmental Biology, 2023
Establishment and maintenance of the primary cilium as a signaling-competent organelle requires a high degree of fine tuning, which is at least in part achieved by a variety of post-translational modifications.
Mariam G. Aslanyan, Cenna Doornbos, Gaurav D. Diwan, Gaurav D. Diwan, Zeinab Anvarian, Tina Beyer, Katrin Junger, Sylvia E. C. van Beersum, Robert B. Russell, Robert B. Russell, Marius Ueffing, Alexander Ludwig, Karsten Boldt, Lotte B. Pedersen, Ronald Roepman   +14 more
doaj   +10 more sources

FOXM1 Protects Against Myocardial Ischemia‐Reperfusion Injury in Rodent and Porcine Models by Suppressing MKRN1‐Dependent LKB1 Ubiquitination [PDF]

Advanced Science
Mitochondrial dysfunction is related to etiopathogenesis and progression of heart failure (HF). The underlying molecular mechanisms are not fully understood. Transcription factor FOXM1 plays an essential role in cardiovascular development.
Shuai Song, Xiaokai Zhang, Zihang Huang, Zhiqiang Pei, Linqi Zeng, Fengze Cai, Tongyao Wang, Mohan Li, Chenyan Liu, Yining Song, Jiahao Guo, Hao Lu, Xinyu Weng, Li Shen, Xiaochun Zhang, Xingxing Cai, Aijun Sun, Junbo Ge   +17 more
doaj   +3 more sources

Ubiquitinome Profiling Reveals in Vivo UBE2D3 Targets and Implicates UBE2D3 in Protein Quality Control. [PDF]

Mol Cell Proteomics, 2023
Ubiquitination has crucial roles in many cellular processes, and dysregulation of ubiquitin machinery enzymes can result in various forms of pathogenesis.
Yalçin Z, Koot D, Bezstarosti K, Salas-Lloret D, Bleijerveld OB, Boersma V, Falcone M, González-Prieto R, Altelaar M, Demmers JAA, Jacobs JJL.   +10 more
europepmc   +15 more sources

Proteome and Ubiquitinome Analyses Reveal the Involvement of Ubiquitination in Resistance to Maize Lethal Necrosis. [PDF]

Mol Plant Pathol
This study analysed the proteome and ubiquitinome of SCMV‐ and/or MCMV‐infected maize plants and demonstrated the role of ZmGOX1 in the maize response to viral infections. ABSTRACT The co‐infection of maize chlorotic mottle virus (MCMV) and sugarcane mosaic virus (SCMV) causes maize lethal necrosis (MLN), which seriously affects the yield and quality ...
Guo H, Dong X, Hao K, Gao X, Guo J, Li J, Zhao S, Sang L, Wang Z, An M, Xia Z, Wu Y.   +11 more
europepmc   +3 more sources

The Proteasomal Deubiquitinating Enzyme PSMD14 Regulates Macroautophagy by Controlling Golgi-to-ER Retrograde Transport [PDF]

Cells, 2020
Ubiquitination regulates several biological processes, however the role of specific members of the ubiquitinome on intracellular membrane trafficking is not yet fully understood.
Hianara A Bustamante, Karina Cereceda, Alexis E González, Guillermo E Valenzuela, Yorka Cheuquemilla, Sergio Hernández, Eloisa Arias-Muñoz, Cristóbal Cerda-Troncoso, Susanne Bandau, Andrea Soza, Gudrun Kausel, Bredford Kerr, Gonzalo A Mardones, Jorge Cancino, Ronald T Hay, Alejandro Rojas-Fernandez, Patricia V Burgos   +16 more
doaj   +4 more sources

Ubiquitinome Profiling Reveals the Landscape of Ubiquitination Regulation in Rice Young Panicles

Genomics, Proteomics & Bioinformatics, 2020
Ubiquitination, an essential post-transcriptional modification (PTM), plays a vital role in nearly every biological process, including development and growth.
Liya Zhu, Han Cheng, Guoqing Peng, Shuansuo Wang, Zhiguo Zhang, Erdong Ni, Xiangdong Fu, Chuxiong Zhuang, Zexian Liu, Hai Zhou   +9 more
doaj   +3 more sources

Data-independent acquisition method for ubiquitinome analysis reveals regulation of circadian biology [PDF]

Nature Communications, 2021
Protein ubiquitylation is often studied by proteomics but how data independent acquisition (DIA) may advance these studies remains to be explored. Here, the authors show that DIA improves ubiquitylation site identification and quantification, enabling ...
Fynn M. Hansen, Maria C. Tanzer, Franziska Brüning, Isabell Bludau, Che Stafford, Brenda A. Schulman, Maria S. Robles, Ozge Karayel, Matthias Mann   +8 more
doaj   +4 more sources

Ubiquitinomics: History, methods, and applications in basic research and drug discovery

PROTEOMICS, Volume 22, Issue 15-16, August 2022., 2022
Abstract The ubiquitin‐proteasome system (UPS) was discovered about 40 years ago and is known to regulate a multitude of cellular processes including protein homeostasis. Ubiquitylated proteins are recognized by downstream effectors, resulting in alterations of protein abundance, activity, or localization. Not surprisingly, the ubiquitylation machinery
Martin Steger, Özge Karayel, Vadim Demichev   +2 more
wiley   +4 more sources