Super Enhanced Purification of Denatured-Refolded Ubiquitinated Proteins by ThUBD Revealed Ubiquitinome Dysfunction in Liver Fibrosis [PDF]
Molecular and Cellular ProteomicsYanchang Li, Jinfang Liu
exaly +2 more sources
Integrative Omics Reveals Glutamine Catabolism-Driven Apoptotic Suppression in Monocytes upon Mechanical Unloading. [PDF]
Adv Sci (Weinh)This research integrated multi‐omics analysis of bone tissue from HLU and control mice revealed that mechanical unloading suppresses intrinsic apoptosis and augments glutamine (Gln) catabolism in osteoclast lineage cells. The findings highlight pivotal roles for SLC1A5‐mediated Gln metabolism and XIAP/Diablo axis‐mediated apoptosis suppression.
Ding Y +14 more
europepmc +2 more sources
Ubiquitinomics: History, methods, and applications in basic research and drug discovery
PROTEOMICS, 2022 Abstract The ubiquitin‐proteasome system (UPS) was discovered about 40 years ago and is known to regulate a multitude of cellular processes including protein homeostasis. Ubiquitylated proteins are recognized by downstream effectors, resulting in alterations of protein abundance, activity, or localization.
Martin Steger +2 more
openaire +3 more sources
Integrative Proteomic Analysis of Multiple Posttranslational Modifications in Inflammatory Response
Genomics, Proteomics & Bioinformatics, 2022 Posttranslational modifications (PTMs) of proteins, particularly acetylation, phosphorylation, and ubiquitination, play critical roles in the host innate immune response. PTMs’ dynamic changes and the crosstalk among them are complicated.
Feiyang Ji +9 more
doaj +1 more source
Ubiquitin proteomics identifies RNA polymerase I as a target of the Smc5/6 complex
Cell Reports, 2023 Summary: Ubiquitination controls numerous cellular processes, and its deregulation is associated with many pathologies. The Nse1 subunit in the Smc5/6 complex contains a RING domain with ubiquitin E3 ligase activity and essential functions in genome ...
Eva Ibars +10 more
doaj +1 more source
Comprehensive analysis of the ubiquitinome during oncogene-induced senescence in human fibroblasts [PDF]
Cell Cycle, 2015 Oncogene-induced senescence (OIS) is an important tumor suppression mechanism preventing uncontrolled proliferation in response to aberrant oncogenic signaling. The profound functional and morphological remodelling of the senescent cell involves extensive changes.
Fee Bengsch +5 more
openaire +2 more sources
Engineering, 2020 Primary hepatocytes (PHCs) are widely used in various fields, but the progressive deterioration of liver-specific features in vitro significantly limits their application.
Zhengyi Jiang +9 more
doaj +1 more source
Mapping the functional importance of site-specific ubiquitination across the human proteome
Abstract Protein ubiquitination regulates cell biology through diverse avenues, from quality control-linked protein degradation to signaling functions such as modulating protein-protein interactions and enzyme activation. To date, hundreds of thousands of ubiquitination sites (ubi-sites) have been identified, however fewer than 1% have ...
van Gerwen J +18 more
europepmc +4 more sources
Detection of the Ubiquitinome in Cells Undergoing Oncogene-Induced Senescence [PDF]
, 2016 Senescent cells exhibit dramatic changes in protein post-translational modifications. Here, we describe a method, stable isotope labeling with amino acids in cell culture (SILAC) coupled to liquid chromatography tandem mass spectrometry (LC-MS/MS), to identify changes in the ubiquitinome in cells that have undergone oncogene-induced senescence.
Hengrui, Zhu +4 more
openaire +2 more sources
Ubiquitous Ubiquitin: The K63 Ubiquitinome [PDF]
The Plant Cell, 2019 Polyubiquitination, the sequential attachment of the small 8 kDa globular protein ubiquitin (Ub) to target proteins, represents a major posttranslational modification that ultimately determines the substrate’s cellular fate. Ub conjugation is an extremely versatile form of protein regulation,
openaire +3 more sources