Use of a High-Affinity Ubiquitin-Binding Domain to Detect and Purify Ubiquitinated Substrates and Their Interacting Proteins [PDF]
Bio-ProtocolOtUBD is a high-affinity ubiquitin-binding domain (UBD) derived from a large protein produced by the microorganism Orientia tsutsugamushi. The following protocol describes a step-by-step process for the enrichment of ubiquitinated proteins from baker'
Nitu Saha +2 more
doaj +2 more sources
Nature Communications, 2022 Here the authors combine multi-omics approaches to uncover a role for ubiquitination and the ubiquitin ligase NEDD4 in targeting the actin binding protein Profilin 1 to regulate actin polymerisation in neural crest cells.
Iman Lohraseb +12 more
doaj +5 more sources
Integrative Omics Reveals Glutamine Catabolism-Driven Apoptotic Suppression in Monocytes upon Mechanical Unloading. [PDF]
Adv Sci (Weinh)This research integrated multi‐omics analysis of bone tissue from HLU and control mice revealed that mechanical unloading suppresses intrinsic apoptosis and augments glutamine (Gln) catabolism in osteoclast lineage cells. The findings highlight pivotal roles for SLC1A5‐mediated Gln metabolism and XIAP/Diablo axis‐mediated apoptosis suppression.
Ding Y +14 more
europepmc +2 more sources
System-wide identification of novel de-ubiquitination targets for USP10 in gastric cancer metastasis through multi-omics screening [PDF]
BMC CancerObjective Ubiquitin-specific peptidase 10 (USP10), a typical de-ubiquitinase, has been found to play a double-edged role in human cancers. Previously, we reported that the expression of USP10 was negatively correlated with the depth of gastric wall ...
Zhi Zeng +12 more
doaj +2 more sources
Root Membrane Ubiquitinome under Short-Term Osmotic Stress [PDF]
International Journal of Molecular Sciences, 2021 Osmotic stress can be detrimental to plants, whose survival relies heavily on proteomic plasticity. Protein ubiquitination is a central post-translational modification in osmotic-mediated stress. In this study, we used the K-Ɛ-GG antibody enrichment method integrated with high-resolution mass spectrometry to compile a list of 719 ubiquitinated lysine ...
Berger, Nathalie +9 more
openaire +5 more sources
Super Enhanced Purification of Denatured-Refolded Ubiquitinated Proteins by ThUBD Revealed Ubiquitinome Dysfunction in Liver Fibrosis [PDF]
Molecular and Cellular ProteomicsXinyu Cheng, Yanchang Li
exaly +2 more sources
Dynamic Changes to the Skeletal Muscle Proteome and Ubiquitinome Induced by the E3 Ligase, ASB2β [PDF]
Molecular & Cellular Proteomics, 2021 Ubiquitination is a posttranslational protein modification that has been shown to have a range of effects, including regulation of protein function, interaction, localization, and degradation. We have previously shown that the muscle-specific ubiquitin E3 ligase, ASB2β, is downregulated in models of muscle growth and that overexpression ASB2β is ...
Craig A. Goodman +4 more
openaire +5 more sources
Unveiling ubiquitinome rearrangements induced by Salmonella infection [PDF]
Autophagy, 2016 Ubiquitination plays a critical role in the activation of host immune responses to infection and serves as a signal for pathogen delivery to phagophores along the xenophagy pathway. We recently performed systematic ubiquitination site profiling of epithelial cells infected with Salmonella Typhimurium.
Bionda, Tihana, Behrends, Christian
openaire +3 more sources
Integrative Proteomic Analysis of Multiple Posttranslational Modifications in Inflammatory Response
Genomics, Proteomics & Bioinformatics, 2022 Posttranslational modifications (PTMs) of proteins, particularly acetylation, phosphorylation, and ubiquitination, play critical roles in the host innate immune response. PTMs’ dynamic changes and the crosstalk among them are complicated.
Feiyang Ji +9 more
doaj +1 more source
Comprehensive analysis of the ubiquitinome during oncogene-induced senescence in human fibroblasts [PDF]
Cell Cycle, 2015 Oncogene-induced senescence (OIS) is an important tumor suppression mechanism preventing uncontrolled proliferation in response to aberrant oncogenic signaling. The profound functional and morphological remodelling of the senescent cell involves extensive changes.
Fee Bengsch +5 more
openaire +2 more sources