Results 31 to 40 of about 760 (147)

Aspirin inhibits proteasomal degradation and promotes α-synuclein aggregate clearance through K63 ubiquitination [PDF]

Nature Communications
Aspirin is a potent lysine acetylation inducer, but its impact on lysine ubiquitination and ubiquitination-directed protein degradation is unclear. Herein, we develop the reversed-pulsed-SILAC strategy to systematically profile protein degradome in ...
Jing Gao, Yang Liu, Chenfang Si, Rui Guo, Shouqiao Hou, Xiaosong Liu, Houfang Long, Di Liu, Daichao Xu, Zai-Rong Zhang, Cong Liu, Bing Shan, Christoph W. Turck, Kaiwen He, Yaoyang Zhang   +14 more
doaj   +2 more sources

USP5 Promotes Ripretinib Resistance in Gastrointestinal Stromal Tumors by MDH2 Deubiquition

Advanced Science
Ripretinib, a broad‐spectrum inhibitor of the KIT and PDGFRA receptor tyrosine kinases, is designated as a fourth‐line treatment for gastrointestinal stromal tumor (GIST). It is tailored for patients resistant to imatinib, sunitinib, and regorafenib.
Haoyu Sun, Zhiwei Cui, Chao Li, Zhishuang Gao, Jun Xu, Yibo Bian, Tianhao Gu, Jianan Zhang, Tengyun Li, Qianzheng Zhou, Dinghua Yang, Zhongyuan He, Bowen Li, Fengyuan Li, Zekuan Xu, Hao Xu   +15 more
doaj   +2 more sources

Use of a High-Affinity Ubiquitin-Binding Domain to Detect and Purify Ubiquitinated Substrates and Their Interacting Proteins

Bio-Protocol
OtUBD is a high-affinity ubiquitin-binding domain (UBD) derived from a large protein produced by the microorganism Orientia tsutsugamushi. The following protocol describes a step-by-step process for the enrichment of ubiquitinated proteins from baker'
Nitu Saha, Mengwen Zhang, Mark Hochstrasser   +2 more
doaj   +2 more sources

Global ubiquitinome profiling identifies NEDD4 as a regulator of Profilin 1 and actin remodelling in neural crest cells [PDF]

Nature Communications, 2022
Here the authors combine multi-omics approaches to uncover a role for ubiquitination and the ubiquitin ligase NEDD4 in targeting the actin binding protein Profilin 1 to regulate actin polymerisation in neural crest cells.
Iman Lohraseb, Peter McCarthy, Genevieve Secker, Ceilidh Marchant, Jianmin Wu, Naveid Ali, Sharad Kumar, Roger J. Daly, Natasha L. Harvey, Hiroshi Kawabe, Oded Kleifeld, Sophie Wiszniak, Quenten Schwarz   +12 more
doaj   +2 more sources

A computational tool to infer enzyme activity using post-translational modification profiling data [PDF]

Communications Biology
Enzymes play a pivotal role in orchestrating complex cellular responses to external stimuli and environmental changes through signal transduction pathways.
Dehui Kong, Aijun Zhang, Ling Li, Zuo-Fei Yuan, Yingxue Fu, Long Wu, Ashutosh Mishra, Anthony A. High, Junmin Peng, Xusheng Wang   +9 more
doaj   +2 more sources

HSP27 Provides Stress-Adaptive Proteostasis Buffering in Glioblastoma and Is Therapeutically Vulnerable [PDF]

Cells
Glioblastoma, isocitrate dehydrogenase (IDH)-wildtype (GBM), remains one of the most aggressive primary brain tumors, and its proteostasis machinery represents a potential therapeutic target.
Yuexia Jiao, Qi Liu, Yuchun Zhang, Xiuyuan Wang, Xinjun Liang, Shaozhong Wei, Zijian Tang   +6 more
doaj   +2 more sources

Proteogenomic reprogramming to a functional human blastomere-like stem cell state via a PARP-DUX4 regulatory axis [PDF]

Cell Reports
Summary: Here, we show that conventional human pluripotent stem cells cultured with non-specific tankyrase-PARP1-inhibited conditions underwent proteogenomic reprogramming to functional blastomere-like tankyrase/PARP inhibitor-regulated naive stem cells (
Ludovic Zimmerlin, Ariana Angarita, Tea Soon Park, Rebecca Evans-Moses, Justin Thomas, Sirui Yan, Isabel Uribe, Isabella Vegas, Clara Kochendoerfer, Willem Buys, Anthony K.L. Leung, Elias T. Zambidis   +11 more
doaj   +2 more sources

System-wide identification of novel de-ubiquitination targets for USP10 in gastric cancer metastasis through multi-omics screening [PDF]

BMC Cancer
Objective Ubiquitin-specific peptidase 10 (USP10), a typical de-ubiquitinase, has been found to play a double-edged role in human cancers. Previously, we reported that the expression of USP10 was negatively correlated with the depth of gastric wall ...
Zhi Zeng, Yina Li, Heng Zhou, Mingyang Li, Juan Ye, Dan Li, Yuxi Zhu, Yonggang Zhang, Xu Zhang, Yunchao Deng, Juan Li, Lijuan Gu, Jie Wu   +12 more
doaj   +2 more sources

Proteome-wide Ubiquitinome Profiling Reveals Substrate-specific Dynamics Within the USP7 Network. [PDF]

Mol Cell Proteomics
ABSTRACT USP7 is a pleiotropic deubiquitylating enzyme that is involved in tumor suppression, (neuro)development, chromatin regulation and the DNA damage response. How USP7 regulates these diverse pathways is still unclear. Here, we report data-independent acquisition and label free quantitation mass spectrometry (DIA-LFQ-MS) to profile
Wolf van der Meer J, van der Knaap JA, Sijm A, Bezstarosti K, Dekkers DHW, Doff WAS, Demmers JAA, Verrijzer CP.   +7 more
europepmc   +4 more sources

Interactome and Ubiquitinome Analyses Identify Functional Targets of Herpes Simplex Virus 1 Infected Cell Protein 0

Frontiers in Microbiology, 2022
Herpes simplex virus 1 (HSV-1) can productively infect multiple cell types and establish latent infection in neurons. Infected cell protein 0 (ICP0) is an HSV-1 E3 ubiquitin ligase crucial for productive infection and reactivation from latency.
Fujun Hou, Fujun Hou, Zeyu Sun, Zeyu Sun, Yue Deng, Yue Deng, Siyu Chen, Siyu Chen, Xiyuan Yang, Xiyuan Yang, Feiyang Ji, Menghao Zhou, Keyi Ren, Dongli Pan, Dongli Pan   +14 more
doaj   +1 more source