Light‐Activatable Ubiquitin for Studying Linkage‐Specific Ubiquitin Chain Formation Kinetics [PDF]
Advanced ScienceUbiquitination is a dynamic post‐translational modification governing protein abundance, function, and localization in eukaryotes. The Ubiquitin protein is conjugated to lysine residues of target proteins, but can also repeatedly be ubiquitinated itself,
Sudakshina Banerjee +7 more
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BOULE is Essential for the Dynamic Disassembly of Heat Shock Granules in Male Germ Cells [PDF]
Advanced ScienceHeat shock triggers stress granule (SG) formation, yet their dynamics in mammalian germ cells remain unclear. Through systematic analysis, we uncovered distinct heat shock granule (HSG) composition and stage‐specific assembly/disassembly in the mouse ...
Xin Li +8 more
doaj +4 more sources
Proteome and Ubiquitinome Analyses Reveal the Involvement of Ubiquitination in Resistance to Maize Lethal Necrosis [PDF]
Molecular Plant PathologyThis study analysed the proteome and ubiquitinome of SCMV‐ and/or MCMV‐infected maize plants and demonstrated the role of ZmGOX1 in the maize response to viral infections. ABSTRACT The co‐infection of maize chlorotic mottle virus (MCMV) and sugarcane mosaic virus (SCMV) causes maize lethal necrosis (MLN), which seriously affects the yield and quality ...
Zihao Xia, Mengnan An, Kaiqiang Hao
exaly +5 more sources
Data-independent acquisition method for ubiquitinome analysis reveals regulation of circadian biology [PDF]
Nature Communications, 2021 Protein ubiquitylation is often studied by proteomics but how data independent acquisition (DIA) may advance these studies remains to be explored. Here, the authors show that DIA improves ubiquitylation site identification and quantification, enabling ...
Fynn M. Hansen +8 more
doaj +5 more sources
Insights into ubiquitinome dynamics in the host‒pathogen interplay during Francisella novicida infection [PDF]
Cell Communication and SignalingUbiquitination functions as an important posttranslational modification for orchestrating inflammatory immune responses and cell death during pathogenic infection.
Luyu Yang +4 more
doaj +4 more sources
A multilevel investigation to reveal the regulatory mechanism of lignin accumulation in juice sac granulation of pomelo [PDF]
BMC Plant BiologyGranulation of juice sacs is a physiological disorder, which affects pomelo fruit quality. Here, the transcriptome and ubiquitinome of the granulated juice sacs were analyzed in Guanxi pomelo.
Luning Liu +10 more
doaj +3 more sources
FOXM1 Protects Against Myocardial Ischemia‐Reperfusion Injury in Rodent and Porcine Models by Suppressing MKRN1‐Dependent LKB1 Ubiquitination [PDF]
Advanced ScienceMitochondrial dysfunction is related to etiopathogenesis and progression of heart failure (HF). The underlying molecular mechanisms are not fully understood. Transcription factor FOXM1 plays an essential role in cardiovascular development.
Shuai Song +17 more
doaj +3 more sources
Root Membrane Ubiquitinome under Short-Term Osmotic Stress [PDF]
International Journal of Molecular Sciences, 2021 Osmotic stress can be detrimental to plants, whose survival relies heavily on proteomic plasticity. Protein ubiquitination is a central post-translational modification in osmotic-mediated stress. In this study, we used the K-Ɛ-GG antibody enrichment method integrated with high-resolution mass spectrometry to compile a list of 719 ubiquitinated lysine ...
Berger, Nathalie +9 more
openaire +6 more sources
Unveiling ubiquitinome rearrangements induced by Salmonella infection [PDF]
Autophagy, 2016 Ubiquitination plays a critical role in the activation of host immune responses to infection and serves as a signal for pathogen delivery to phagophores along the xenophagy pathway. We recently performed systematic ubiquitination site profiling of epithelial cells infected with Salmonella Typhimurium.
Bionda, Tihana, Behrends, Christian
openaire +4 more sources
Dynamic Changes to the Skeletal Muscle Proteome and Ubiquitinome Induced by the E3 Ligase, ASB2β [PDF]
Molecular & Cellular Proteomics, 2021 Ubiquitination is a posttranslational protein modification that has been shown to have a range of effects, including regulation of protein function, interaction, localization, and degradation. We have previously shown that the muscle-specific ubiquitin E3 ligase, ASB2β, is downregulated in models of muscle growth and that overexpression ASB2β is ...
Craig A. Goodman +4 more
openaire +7 more sources