Results 41 to 50 of about 851 (145)

The functional landscape of the human ubiquitinome

Abstract Protein ubiquitination regulates cell biology through diverse avenues, from quality control-linked protein degradation to signaling functions such as modulating protein-protein interactions and enzyme activation. Mass spectrometry-based proteomics has allowed proteome-scale quantification of hundreds of thousands of ubiquitination ...
van Gerwen J, Fottner M, Wang S, Busby B, Boswell E, Schnacke P, Carrano AC, Bakowski MA, Troemel ER, Studer R, Strumillo M, Martin M, Harper JW, Lang K, Jones AR, Bennett EJ, Vizcaíno JA, Barrio-Hernandez I, Beltrao P.   +18 more
europepmc   +2 more sources

BRCA1 mutations in high-grade serous ovarian cancer are associated with proteomic changes in DNA repair, splicing, transcription regulation and signaling [PDF]

, 2022
Ovarian cancer; ProteomicsCáncer de ovarios: ProteómicaCàncer d'ovaris; ProteòmicaDespite recent advances in the management of BRCA1 mutated high-grade serous ovarian cancer (HGSC), the physiology of these tumors remains poorly understood.
Borras, Eva, Bradbury Lobato, Melissa, Castellví Vives, Josep, Gil Moreno, Antonio, Mendez Fernández, Olga, Perez Benavente, Maria Asuncion, Sabidó, Eduard, Santamaria Margalef, Anna, Sánchez Iglesias, José Luis   +8 more
core   +4 more sources

Integrative Proteomic Analysis of Multiple Posttranslational Modifications in Inflammatory Response

Genomics, Proteomics & Bioinformatics, 2022
Posttranslational modifications (PTMs) of proteins, particularly acetylation, phosphorylation, and ubiquitination, play critical roles in the host innate immune response. PTMs’ dynamic changes and the crosstalk among them are complicated.
Feiyang Ji, Menghao Zhou, Huihui Zhu, Zhengyi Jiang, Qirui Li, Xiaoxi Ouyang, Yiming Lv, Sainan Zhang, Tian Wu, Lanjuan Li   +9 more
doaj   +1 more source

Improvement of ubiquitylation site detection by Orbitrap mass spectrometry [PDF]

, 2017
Ubiquitylation is an important posttranslational protein modification that is involved in many cellular events. Immunopurification of peptides containing a K-ε-diglycine (diGly) remnant as a mark of ubiquitylation combined with mass spectrometric ...
Bezstarosti, K. (Karel), Dekkers, D.H. (Dick), Demmers, J.A.A. (Jeroen), Elgersma, Y. (Ype), Mientjes, E.J. (Edwin), Rijkers, E.J., Sap, K.A. (Karen), van der Wal, L. (Lennart)   +7 more
core   +6 more sources

Siah2 control of T-regulatory cells limits anti-tumor immunity. [PDF]

, 2020
Understanding the mechanisms underlying anti-tumor immunity is pivotal for improving immune-based cancer therapies. Here, we report that growth of BRAF-mutant melanoma cells is inhibited, up to complete rejection, in Siah2-/- mice.
Aifantis, Ioannis, Bosenberg, Marcus, Bradley, Linda M, Brown, Kevin, Dolgalev, Igor, Feng, Yongmei, Hockemeyer, Kathryn, Li, Yan, Marine, Jean-Christophe, Otero, Dennis C, Poźniak, Joanna, Rambow, Florian, Ronai, Ze'ev A, Scortegagna, Marzia, Tinoco, Roberto, Zhang, Tongwu   +15 more
core   +2 more sources

Site-Specific K63 Ubiquitinomics Provides Insights into Translation Regulation under Stress [PDF]

Journal of Proteome Research, 2018
During oxidative stress, K63-linked polyubiquitin chains modify a variety of proteins including ribosomes. Knowledge of the precise sites of K63 ubiquitin is key to understand its function during the response to stress. To identify the sites of K63 ubiquitin, we developed a new mass spectrometry based method that quantified >1100 K63 ubiquitination ...
Songhee Back, Andrew W. Gorman, Christine Vogel, Gustavo M. Silva   +3 more
openaire   +2 more sources

Super Enhanced Purification of Denatured-Refolded Ubiquitinated Proteins by ThUBD Revealed Ubiquitinome Dysfunction in Liver Fibrosis. [PDF]

Mol Cell Proteomics
Ubiquitination is crucial for maintaining protein homeostasis and plays a vital role in diverse biological processes. Ubiquitinome profiling and quantification are of great scientific significance. Artificial ubiquitin-binding domains (UBDs) have been widely employed to capture ubiquitinated proteins.
Cheng X, Wang Y, Liu J, Wu Y, Zhang Z, Liu H, Tian L, Zhang L, Chang L, Xu P, Zhang L, Li Y.   +11 more
europepmc   +3 more sources

Dynamic Changes to the Skeletal Muscle Proteome and Ubiquitinome Induced by the E3 Ligase, ASB2β [PDF]

Molecular & Cellular Proteomics, 2021
Ubiquitination is a posttranslational protein modification that has been shown to have a range of effects, including regulation of protein function, interaction, localization, and degradation. We have previously shown that the muscle-specific ubiquitin E3 ligase, ASB2β, is downregulated in models of muscle growth and that overexpression ASB2β is ...
Craig A. Goodman, Jonathan R. Davey, Adam Hagg, Benjamin L. Parker, Paul Gregorevic   +4 more
openaire   +3 more sources

Ubiquitin proteomics identifies RNA polymerase I as a target of the Smc5/6 complex

Cell Reports, 2023
Summary: Ubiquitination controls numerous cellular processes, and its deregulation is associated with many pathologies. The Nse1 subunit in the Smc5/6 complex contains a RING domain with ubiquitin E3 ligase activity and essential functions in genome ...
Eva Ibars, Joan Codina-Fabra, Gemma Bellí, Celia Casas, Marc Tarrés, Roger Solé-Soler, Neus P. Lorite, Pilar Ximénez-Embún, Javier Muñoz, Neus Colomina, Jordi Torres-Rosell   +10 more
doaj   +1 more source

Advances in data‐independent acquisition mass spectrometry towards comprehensive digital proteome landscape

Mass Spectrometry Reviews, Volume 42, Issue 6, Page 2324-2348, November/December 2023., 2023
Abstract The data‐independent acquisition mass spectrometry (DIA‐MS) has rapidly evolved as a powerful alternative for highly reproducible proteome profiling with a unique strength of generating permanent digital maps for retrospective analysis of biological systems.
Reta Birhanu Kitata, Jhih‐Ci Yang, Yu‐Ju Chen   +2 more
wiley   +1 more source