Results 81 to 90 of about 97,609 (305)
IMACulate(DE3), an E. coli Strain for High Purity His‐Tagged Protein Purifications
Biotechnology and Bioengineering, EarlyView.ABSTRACT Immobilised Metal Affinity Chromatography (IMAC) is widely used to purify his‐tagged recombinant proteins from Escherichia coli. However, endogenous contaminants with histidine clusters, such as GFAT and PDH E1 proteins, are often co‐purified with the target protein.
Jia Q. Truong +5 more
wiley +1 more source
SPES3 Facility RELAP5 Sensitivity Analyses on the Containment System for Design Review
Science and Technology of Nuclear Installations, 2012 An Italian MSE R&D programme on Nuclear Fission is funding, through ENEA, the design and testing of SPES3 facility at SIET, for IRIS reactor simulation.
Andrea Achilli +6 more
doaj +1 more source
A Modular Nucleoside Kinase Cascade for the Synthesis of Ribonucleoside Triphosphates
Biotechnology and Bioengineering, EarlyView.Damm et al. report standardized enzymatic cascades for production of all canonical nucleoside triphosphates from the corresponding nucleosides, using kinase‐catalyzed phosphorylation from acetyl phosphate. Reactions afforded ∼ 100 mM product in near‐quantitative conversion.
Oliver T. Damm +3 more
wiley +1 more source
, 2008 Xanthomonas albilineans produces a UDP-glucose dehydrogenase growing on sucrose. The enzyme oxidizes UDP-glucose to UDP-glucuronic acid by using molecular oxygen and NADPH.
Legaz González, María Estrella +2 more
core +1 more source
Characterization and downstream mannose phosphorylation of human recombinant α-L-iduronidase produced in Arabidopsis complex glycan-deficient (cgl) seeds [PDF]
, 2013 This work was supported by a Wellcome Trust award to TMG.Mucopolysaccharidosis (MPS) I is a lysosomal storage disease caused by a deficiency of α-L-iduronidase (IDUA) (EC 3.2.1.76); enzyme replacement therapy is the conventional treatment for this ...
Brooks, Doug +26 more
core +1 more source
β‐1,4‐galactosyltransferase 1 inhibitors modulate cellular N‐glycan profiles
Biotechnology Progress, EarlyView.Graphical representation of the study workflow used to investigate the effects of known in vitro galactosyltransferase inhibitors on cell culture performance and recombinant IgG galactosylation. Abstract N‐glycosylation is a key post‐translational modification of proteins influencing their physicochemical properties as well as biological activity. This
Jaka Kranjc +4 more
wiley +1 more source
Litz-Lab/Scarab-infra: Scarab release for UDP ISCA artifact evaluation
<p>Release of ISCA2024-UDP branch for ISCA2024 UDP Artifact</p> <p><strong>Full Changelog</strong>: https://github.com/Litz-Lab/Scarab-infra/compare/v.1.0.1...v.1.0.1-udp</p ...
cchen258, anlhu, Surim Oh
core +1 more source
PharmVar GeneFocus: CYP1A2—Clinical Impact, Genetic Variation, and Updated Nomenclature
Clinical Pharmacology &Therapeutics, EarlyView.The Pharmacogene Variation Consortium (PharmVar) provides nomenclature for the highly polymorphic human CYP1A2 gene. CYP1A2 plays a crucial role in the biotransformation of several commonly used drugs, including antipsychotics, antidepressants, anxiolytics, and methylxanthines.
Katalin Monostory +13 more
wiley +1 more source
PLoS Neglected Tropical Diseases, 2015 Interconversion of UDP-glucose (UDP-Glc) and UDP-galactose (UDP-Gal) by the UDP-Glc 4´-epimerase intimately connects the biosynthesis of these two nucleotide sugars. Their de novo biosynthesis involves transformation of glucose-6-phosphate into glucose-1-
Sebastian Damerow +7 more
doaj +1 more source
Frontiers in Psychology, 2014 Argumentation is a crucial component of our lives. Although in the absence of rational debate our legal, political, and scientific systems would not be possible, there is still no integrated area of research on the psychology of argumentation ...
Marco eLillo-Unglaube +3 more
doaj +1 more source