Human 2'-deoxynucleoside 5'-phosphate N-hydrolase 1 : mechanism of 2'-deoxyuridine 5'-monophosphate hydrolysis [PDF]
, 2023 Funding: Industrial Biotechnology Innovation Centre - 2021-01-01.The enzyme 2′-deoxynucleoside 5′-phosphate N-hydrolase 1 (DNPH1) catalyzes the N-ribosidic bond cleavage of 5-hydroxymethyl-2′-deoxyuridine 5′-monophosphate to generate 2-deoxyribose 5 ...
Carberry, Anna Ellen +5 more
core +1 more source
Viruses with U-DNA : New Avenues for Biotechnology [PDF]
, 2021 Deoxyuridine in DNA has recently been in the focus of research due to its intriguing roles in several physiological and pathophysiological situations. Although not an orthodox DNA base, uracil may appear in DNA via either cytosine deamination or thymine ...
Nagy, Kinga K. +2 more
core +3 more sources
The Verrucomicrobia LexA-binding Motif: Insights into the Evolutionary Dynamics of the SOS Response
Frontiers in Molecular Biosciences, 2016 The SOS response is the primary bacterial mechanism to address DNA damage, coordinating multiple cellular processes that include DNA repair, cell division and translesion synthesis.
Ivan Erill +3 more
doaj +1 more source
Structure and stereochemistry of the base excision repair glycosylase MutY reveal a mechanism similar to retaining glycosidases. [PDF]
, 2015 MutY adenine glycosylases prevent DNA mutations by excising adenine from promutagenic 8-oxo-7,8-dihydroguanine (OG):A mismatches. Here, we describe structural features of the MutY active site bound to an azaribose transition state analog which indicate a
Cao, Sheng +6 more
core +2 more sources
The FEBS Journal, Volume 287, Issue 20, Page 4401-4414, October 2020., 2020 We performed alanine‐scanning mutagenesis of the extracellular loops of the yeast lysine transporter Lyp1. We find positions that affect either the localization or activity of Lyp1. Half of the affected mutants are located in the extension of extracellular loop 3 or in a predicted α‐helix in extracellular loop 4. We conclude that extracellular loops of
Joury S. van‘t Klooster +6 more
wiley +1 more source
Small multicopy, non-integrative shuttle vectors based on the plasmid pRN1 for Sulfolobus acidocaldarius and Sulfolobus solfataricus, model organisms of the (cren-)archaea [PDF]
, 2007 The extreme thermoacidophiles of the genus Sulfolobus are among the best-studied archaea but have lacked small, reliable plasmid vectors, which have proven extremely useful for manipulating and analyzing genes in other microorganisms.
Albers, Sonja-Verena +3 more
core +4 more sources
C-Nucleoside Formation in the Biosynthesis of the Antifungal Malayamycin A. [PDF]
, 2019 Malayamycin A is an unusual bicyclic C-nucleoside, with interesting antiviral, antifungal, and anticancer bioactivity. We report here the discovery and characterization of the biosynthetic pathway to malayamycin by using genome mining of near-identical ...
core +2 more sources
On the Sensitivity of Protein Data Bank Normal Mode Analysis: An Application to GH10 Xylanases [PDF]
Phys. Biol. 12 (2015) 066013, 2015 Protein data bank entries obtain distinct, reproducible flexibility characteristics determined by normal mode analyses of their three dimensional coordinate files. We study the effectiveness and sensitivity of this technique by analyzing the results on one class of glycosidases: family 10 xylanases.
arxiv +1 more source
USER fusion: a rapid and efficient method for simultaneous fusion and cloning of multiple PCR products [PDF]
, 2007 We present a method that allows simultaneous fusion and cloning of multiple PCR products in a rapid and efficient manner. The procedure is based on the use of PCR primers that contain a single deoxyuridine residue near their 5′ end.
Geu-Flores, Fernando +3 more
core +2 more sources
New DNA Repair and Demethylation Functions in Uracil DNA Glycosylase Superfamily [PDF]
, 2023 Uracil-DNA glycosylase (UDG) superfamily, which consists of several groups of enzymes that recognize the damaged DNA bases and initiate the base excision repair (BER) pathway, is most important in dealing with DNA deamination and other base modifications.
Chang, Chenyan
core +1 more source