Results 21 to 30 of about 8,610 (171)

Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells [PDF]

, 2015
Chinese hamster ovary (CHO) cells are widely used as cell factories for the production of biopharmaceuticals. In contrast to the highly optimized production processes for monoclonal antibody (mAb)-based biopharmaceuticals, improving productivity of non ...
Andersen, Mikael Rørdam, Grav, Lise Marie, Hansen, Henning Gram, Kildegaard, Helene Faustrup, Kol, Stefan, Lund, Anne Mathilde, Lundqvist, Magnus, Min Lee, Gyun, Nilsson, Claes Nymand, Rockberg, Johan   +9 more
core   +1 more source

Human 2'-deoxynucleoside 5'-phosphate N-hydrolase 1 : mechanism of 2'-deoxyuridine 5'-monophosphate hydrolysis [PDF]

, 2023
Funding: Industrial Biotechnology Innovation Centre - 2021-01-01.The enzyme 2′-deoxynucleoside 5′-phosphate N-hydrolase 1 (DNPH1) catalyzes the N-ribosidic bond cleavage of 5-hydroxymethyl-2′-deoxyuridine 5′-monophosphate to generate 2-deoxyribose 5 ...
Carberry, Anna Ellen, da Silva, R.G., Devi, Suneeta, Dickson, Alison, Harrison, David James, Zickuhr, Greice Michele   +5 more
core   +1 more source

Extracellular loops matter – subcellular location and function of the lysine transporter Lyp1 from Saccharomyces cerevisiae

The FEBS Journal, Volume 287, Issue 20, Page 4401-4414, October 2020., 2020
We performed alanine‐scanning mutagenesis of the extracellular loops of the yeast lysine transporter Lyp1. We find positions that affect either the localization or activity of Lyp1. Half of the affected mutants are located in the extension of extracellular loop 3 or in a predicted α‐helix in extracellular loop 4. We conclude that extracellular loops of
Joury S. van‘t Klooster, Frans Bianchi, Ruben B. Doorn, Mirco Lorenzon, Jarnick H. Lusseveld, Christiaan M. Punter, Bert Poolman   +6 more
wiley   +1 more source

A Versatile System for USER Cloning-Based Assembly of Expression Vectors for Mammalian Cell Engineering [PDF]

, 2014
A new versatile mammalian vector system for protein production, cell biology analyses, and cell factory engineering was developed. The vector system applies the ligation-free uracil-excision based technique--USER cloning--to rapidly construct mammalian ...
A Aronheim, Anne Mathilde Lund, BG Hansen, Bjarne Gram Hansen, C Aslanidis, D Kalderon, DG Gibson, F Geu-Flores, FM Wurm, G Walsh, G Walsh, Helene Faustrup Kildegaard, HH Nour-Eldin, HH Nour-Eldin, J Bitinaite, J Burke, J Roth, JF Hancock, Julie Rank, L Fliegel, Maja Borup Kjær Petersen, Mark Isalan, MH Nørholm, Mikael Rørdam Andersen, MY Zhou, NE Lewis, PW Gray, PW Gray, R Rizzuto, S Munro, S Munro, SJ Gould, SS Tate, T Ellis, T Smith, Uffe Hasbro Mortensen, X Xu, Y Durocher   +37 more
core   +3 more sources

The glycine-rich motif of Pyrococcus abyssi DNA polymerase D is critical for protein stability. [PDF]

, 2010
En libre-accès sur Archimer : http://archimer.ifremer.fr/doc/00002/11293/7879.pdfInternational audienceA glycine-rich motif described as being involved in human polymerase delta proliferating cell nuclear antigen (PCNA) binding has also been identified ...
Castrec, Benoît, Flament, Didier, Henneke, Ghislaine, Laurent, Sébastien, Raffin, Jean-Paul   +4 more
core   +2 more sources

Toward high-resolution population genomics using archaeological samples [PDF]

, 2016
The term ‘ancient DNA’ (aDNA) is coming of age, with over 1,200 hits in the PubMed database, beginning in the early 1980s with the studies of ‘molecular paleontology’.
Alexander S. Mikheyev, Ancha Baranova, Cano, Egor Prokhortchouk, Elhaik, Eran Elhaik, Evgeny Rogaev, Falush, GaneshPrasad ArunKumar, Hosseinali Asgharian, Irina Morozova, Li, Lindahl, Meyer, Nerlich, Olalde, Pavel Flegontov, Petr Ponomarenko, Sergey Bruskin, Tatiana V. Tatarinova, Taubenberger, Veeramah, Vladimir Klyuchnikov, Yuri Nikolsky, Yuriy Gankin   +24 more
core   +2 more sources

A New Class of Uracil–DNA Glycosylase Inhibitors Active against Human and Vaccinia Virus Enzyme

Molecules, 2021
Uracil–DNA glycosylases are enzymes that excise uracil bases appearing in DNA as a result of cytosine deamination or accidental dUMP incorporation from the dUTP pool. The activity of Family 1 uracil–DNA glycosylase (UNG) activity limits the efficiency of
I. Grin, G. V. Mechetin, Rustem D. Kasymov, Evgeniia A. Diatlova, A. Yudkina, S. Shchelkunov, I. Gileva, A. A. Denisova, G. Stepanov, G. Chilov, D. Zharkov   +10 more
semanticscholar   +1 more source

A structurally conserved motif in γ-herpesvirus uracil-DNA glycosylases elicits duplex nucleotide-flipping [PDF]

, 2018
Efficient γ-herpesvirus lytic phase replication requires a virally encoded UNG-type uracil-DNA glycosylase as a structural element of the viral replisome.
Bagneris, Claire, Barrett, Tracey, Cole, Ambrose, Earl, Chris, Savva, Renos, Zeman, K.   +5 more
core   +2 more sources

Repair of APOBEC3G-Mutated Retroviral DNA In Vivo Is Facilitated by the Host Enzyme Uracil DNA Glycosylase 2

bioRxiv, 2021
While APOBEC3-mediated mutation of retroviruses is well-established, what role the host base excision repair enzymes play in correcting these mutations is not clear. This question is especially difficult to address in vivo. ABSTRACT Apolipoprotein B mRNA
Karen Salas-Briceno, S. Ross
semanticscholar   +1 more source

Restriction-modification system with methyl-inhibited base excision and abasic-site cleavage activities [PDF]

, 2015
The restriction-modification systems use epigenetic modification to distinguish between self and nonself DNA. A modification enzyme transfers a methyl group to a base in a specific DNA sequence while its cognate restriction enzyme introduces breaks in ...
Fukuyo, Masaki, Furuta, Yoshikazu, Hamakawa, Takeshi, Ide, Hiroshi, Ishikawa, Ken, Kobayashi, Ichizo, Nakano, Toshiaki, Watanabe-Matsui, Miki, Yano, Hirokazu, Zhang, Yingbiao   +9 more
core   +2 more sources