Results 21 to 30 of about 1,064 (75)

Targeting uracil-DNA glycosylases for therapeutic outcomes using insights from virus evolution [PDF]

, 2019
Ung-type uracil-DNA glycosylases are frontline defenders of DNA sequence fidelity in bacteria, plants, and animals; Ungs also directly assist both innate and humoral immunity.
Bouhamdan M, Chamberlin M, Friedberg EC, Katz GE, Le Rouzic E, Lindahl T, Lovett PS, Mahler I, Maisch WF, Price AR, Pyles RB, Renos Savva, Rhoades M, Stuart DT, Tomita F, Tomita F, Wang Z, Warner HR, Willetts KE   +18 more
core   +1 more source

Analysis of the impact of a uracil DNA glycosylase attenuated in AP-DNA binding in maintenance of the genomic integrity in Escherichia coli [PDF]

, 2010
Uracil DNA glycosylase (Ung) initiates the uracil excision repair pathway. We have earlier characterized the Y66W and Y66H mutants of Ung and shown that they are compromised by ∼7- and ∼170-fold, respectively in their uracil excision activities.
Acharya, Acharya, Aravind, Barrett, Blaisdell, Blattner, Caradonna, Cone, Datsenko, Demple, Dianov, Dianov, Domena, Emsley, Fels, Friedberg, Graves, Guillet, Handa, Handa, Handa, Imlay, Kavli, Krokan, Krusong, Lancaster, Lin, Lindahl, Lindahl, Lindahl, Lindahl, Loeb, Lu, Miller, Otterlei, Parikh, Payne, Pope, Putnam, Ravishankar, Reid, Roy, Sambrook, Sanjay Kumar Bharti, Snowden, Sohail, Spek, Srinath, Talpaert-Borle, Umesh Varshney, Van Der Spoel, Venkatesh, Vieira, Warner, Waters, Williams, Yu, Yu   +57 more
core   +2 more sources

A Versatile System for USER Cloning-Based Assembly of Expression Vectors for Mammalian Cell Engineering [PDF]

, 2014
A new versatile mammalian vector system for protein production, cell biology analyses, and cell factory engineering was developed. The vector system applies the ligation-free uracil-excision based technique--USER cloning--to rapidly construct mammalian ...
A Aronheim, Anne Mathilde Lund, BG Hansen, Bjarne Gram Hansen, C Aslanidis, D Kalderon, DG Gibson, F Geu-Flores, FM Wurm, G Walsh, G Walsh, Helene Faustrup Kildegaard, HH Nour-Eldin, HH Nour-Eldin, J Bitinaite, J Burke, J Roth, JF Hancock, Julie Rank, L Fliegel, Maja Borup Kjær Petersen, Mark Isalan, MH Nørholm, Mikael Rørdam Andersen, MY Zhou, NE Lewis, PW Gray, PW Gray, R Rizzuto, S Munro, S Munro, SJ Gould, SS Tate, T Ellis, T Smith, Uffe Hasbro Mortensen, X Xu, Y Durocher   +37 more
core   +6 more sources

Removal of Misincorporated Ribonucleotides from Prokaryotic Genomes: An Unexpected Role for Nucleotide Excision Repair [PDF]

, 2013
Stringent steric exclusion mechanisms limit the misincorporation of ribonucleotides by high-fidelity DNA polymerases into genomic DNA. In contrast, low-fidelity Escherichia coli DNA polymerase V (pol V) has relatively poor sugar discrimination and ...
A Vaisman, AL Lu, Alexandra Vaisman, AR Fernández de Henestrosa, B Hiller, B Rydberg, B Van Houten, BD Davis, Bennett Van Houten, D Mu, DJ Crowley, DL Croteau, DL Croteau, DM Maron, Donald Huston, E Curti, E Wolff, EA Kouzminova, EF DeRose, EG Frank, EG Frank, EW Hou, F Lazzaro, G Churchward, G Jiang, GF Moolenaar, H Krokan, H Ohmori, H Shinagawa, H Wang, IJ Fijalkowska, J Yang, JA Brown, JB Sweasy, JC Huang, JE Sale, JG Moggs, JJ Lin, JJ Truglio, JL Sparks, John P. McDonald, JP McDonald, K Sugasawa, L Garibyan, L Zheng, LA Christensen, Lili Liu, M Sandigursky, M Skorvaga, M Tang, M Watanabe-Akanuma, M Yamaguchi, MA Reijns, MC Wahl, ME Branum, MM Ghodgaonkar, MR Albertella, N Kim, Q Jiang, Q Ruan, R Cone, R Woodgate, RJ Sanderson, Roger Woodgate, RP Cunningham, SA Lujan, SA Nick McElhinny, SA Nick McElhinny, Sue Jinks-Robertson, T Baba, T Lindahl, T Lindahl, T Nakano, W Kuban, W Yang, Wojciech Kuban, Y Shen, YW Kow   +77 more
core   +3 more sources

Inducible and constitutive promoters for genetic systems in Sulfolobus acidocaldarius [PDF]

, 2010
Central to genetic work in any organism are the availability of a range of inducible and constitutive promoters. In this work we studied several promoters for use in the hyperthermophilic archaeon Sulfolobus acidocaldarius. The promoters were tested with
AF Andersson, Alexander Wlodkowski, B Ehresmann, B Wiedenheft, C Haseltine, C Haseltine, D Barthelme, D Gotz, DW Grogan, G Lipps, Georg Lipps, IG Duggin, JM Lubelska, L Chen, L Deng, M Jonuscheit, M Wagner, MG Elferink, MS Reilly, N Kurosawa, O Wurtzel, Q She, RY Samson, S Berkner, S Berkner, S Berkner, Silvia Berkner, Sonja-Verena Albers, SV Albers, SV Albers, TD Brock, V Hoang, WS Maaty   +32 more
core   +2 more sources

A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering [PDF]

, 2010
Background The combined use of restriction enzymes with PCR has revolutionized molecular cloning, but is inherently restricted by the content of the manipulated DNA sequences.
Nørholm, Morten HH
core   +3 more sources

Persistent increase in ventral hippocampal long‐term potentiation by juvenile stress: A role for astrocytic glutamine synthetase

Glia, Volume 67, Issue 12, Page 2279-2293, December 2019., 2019
Main Points Juvenile stress (JS) increases ventral CA1‐LTP lastingly Blocking glutamine synthetase mimics LTP after JS, providing its product glutamine normalizes it Glutamine–glutamate cycle in astrocytes regionally mediates allostatic plasticity after JS Abstract A traumatic childhood is among the most important risk factors for developing stress ...
Sebastian Ivens, Gürsel Çalışkan, Ismini Papageorgiou, Tiziana Cesetti, Ansgar Malich, Oliver Kann, Uwe Heinemann, Oliver Stork, Anne Albrecht   +8 more
wiley   +1 more source

Expression, Purification and Characterization of Ricin vectors used for exogenous antigen delivery into the MHC Class I presentation pathway [PDF]

, 2003
Disarmed versions of the cytotoxin ricin can deliver fused peptides into target cells leading to MHC class I-restricted antigen presentation [Smith et al. J Immunol 2002; 169:99-107].
Lord, J. Michael, Marsden, Catherine J., Roberts, Lynne M., Smith, Daniel C.   +3 more
core   +3 more sources

Mimicking damaged DNA with a small molecule inhibitor of human UNG2 [PDF]

, 2006
Human nuclear uracil DNA glycosylase (UNG2) is a cellular DNA repair enzyme that is essential for a number of diverse biological phenomena ranging from antibody diversification to B-cell lymphomas and type-1 human immunodeficiency virus infectivity ...
Ccp4, Daniel J. Krosky, Di Noia, Drohat, Drohat, Drohat, Erlanson, Halgunset, Heberling, James T. Stivers, Jiang, Kavli, Krokan, Kurian, L. Mario Amzel, Lauren Seiple, Lindahl, Lindahl, Malet-Martino, Maly, Mario A. Bianchet, Nilsen, Nilsen, Nilsen, Otwinoski, Parikh, Petersen-Mahrt, Priet, Rada, Seiple, Slupphaug, Stivers, Stivers, Suhman Chung, Werner   +34 more
core   +5 more sources

Development of the Multiple Gene Knockout System with One‐Step PCR in Thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius

Archaea, Volume 2017, Issue 1, 2017., 2017
Multiple gene knockout systems developed in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius are powerful genetic tools. However, plasmid construction typically requires several steps. Alternatively, PCR tailing for high‐throughput gene disruption was also developed in S.
Shoji Suzuki, Norio Kurosawa, Sonja-Verena Albers   +2 more
wiley   +1 more source