Results 1 to 10 of about 22,593 (200)

An uracil-linked hydroxyflavone probe for the recognition of ATP [PDF]

Beilstein Journal of Organic Chemistry, 2018
Background: Nucleotides are essential molecules in living systems due to their paramount importance in various physiological processes. In the past years, numerous attempts were made to selectively recognize and detect these analytes, especially ATP ...
Márton Bojtár, Péter Zoltán Janzsó-Berend, Dávid Mester, Dóra Hessz, Mihály Kállay, Miklós Kubinyi, István Bitter   +6 more
doaj   +2 more sources

UdgX-Mediated Uracil Sequencing at Single-Nucleotide Resolution [PDF]

Journal of the American Chemical Society, 2022
As an aberrant base in DNA, uracil is generated by either deoxyuridine (dU) misincorporation or cytosine deamination, and involved in multiple physiological and pathological processes. Genome-wide profiles of uracil are important for study of these processes.
Liudan Jiang, Jiayong Yin, Maoxiang Qian, Shaoqin Rong, Shengqi Zhang, Kejing Chen, Chengchen Zhao, Yuanqing Tan, Jiayin Guo, Hao Chen, Siyun Gao, Tingting Liu, Yi Liu, Bin Shen, Jian Yang, Yong Zhang, Fei-Long Meng, Jinchuan Hu, Honghui Ma, Yi-Han Chen   +19 more
openaire   +2 more sources

Real-time PCR method for detection of short DNA using a deoxyuridine probe and application for detection of fomivirsen

BioTechniques, 2022
This study sought to develop a short DNA detection method using a deoxyuridine probe and polymerase chain reaction. The probe was hybridized to the target short DNA, which was then extended by DNA polymerase.
Naoki Harikai, Yuko Tanaka, Satoshi Miyashita, Kazumasa Zaima, Kazufusa Shinomiya   +4 more
doaj   +1 more source

Sequencing uracil in DNA at single-nucleotide resolution [PDF]

, 2021
ABSTRACTAs an aberrant base in DNA, uracil is generated by dUMP misincorporation or cytosine deamination, and involved in multiple physiological and pathological processes. Current methods for whole-genome mapping of uracil all rely on uracil-DNA N-glycosylase (UNG) and are limited in resolution or specificity. Here, we present a UNG-independent Single-
Yong Liu, Yiqing Chen, Fei-Long Meng, Jiayin Guo, Yin J, Tan Y, Bin Shen, Rong S, Chengchen Zhao, Ma H, Ma H, Siyuan Gao, Jinchuan Hu, Kui Chen, Liu T, Jinxuan Yang, Maoxiang Qian, Jiang L, Jiang L, Yan Zhang, Huaisheng Chen   +20 more
openaire   +2 more sources

Biochemical characterization of uracil phosphoribosyltransferase from Mycobacterium tuberculosis. [PDF]

PLoS ONE, 2013
Uracil phosphoribosyltransferase (UPRT) catalyzes the conversion of uracil and 5-phosphoribosyl-α-1-pyrophosphate (PRPP) to uridine 5'-monophosphate (UMP) and pyrophosphate (PP(i)). UPRT plays an important role in the pyrimidine salvage pathway since UMP
Anne Drumond Villela, Rodrigo Gay Ducati, Leonardo Astolfi Rosado, Carlos Junior Bloch, Maura Vianna Prates, Danieli Cristina Gonçalves, Carlos Henrique Inacio Ramos, Luiz Augusto Basso, Diogenes Santiago Santos   +8 more
doaj   +1 more source

Prior metabolite extraction fully preserves RNAseq quality and enables integrative multi-‘omics analysis of the liver metabolic response to viral infection

RNA Biology, 2023
Here, we provide an in-depth analysis of the usefulness of single-sample metabolite/RNA extraction for multi-‘omics readout. Using pulverized frozen livers of mice injected with lymphocytic choriomeningitis virus (LCMV) or vehicle (Veh), we isolated RNA ...
Zachary B Madaj, Michael S. Dahabieh, Vijayvardhan Kamalumpundi, Brejnev Muhire, J. Pettinga, Rebecca A. Siwicki, Abigail E. Ellis, Christine Isaguirre, Martha L. Escobar Galvis, Lisa DeCamp, Russell G. Jones, Scott A. Givan, Marie Adams, Ryan D. Sheldon   +13 more
doaj   +1 more source

Silencing NTPDase3 activity rehabilitates the osteogenic commitment of post-menopausal stem cell bone progenitors

Stem Cell Research & Therapy, 2023
Background Endogenously released adenine and uracil nucleotides favour the osteogenic commitment of bone marrow-derived mesenchymal stromal cells (BM-MSCs) through the activation of ATP-sensitive P2X7 and UDP-sensitive P2Y6 receptors.
José Bernardo Noronha-Matos, Rui Pinto-Cardoso, Catarina Bessa-Andrês, Maria Teresa Magalhães-Cardoso, Fátima Ferreirinha, Maria Adelina Costa, José Marinhas, Rolando Freitas, Rui Lemos, Adélio Vilaça, António Oliveira, Julie Pelletier, Jean Sévigny, Paulo Correia-de-Sá   +13 more
doaj   +1 more source

Statistical analysis of readthrough levels for nonsense mutations in mammalian cells reveals a major determinant of response to gentamicin. [PDF]

PLoS Genetics, 2012
The efficiency of translation termination depends on the nature of the stop codon and the surrounding nucleotides. Some molecules, such as aminoglycoside antibiotics (gentamicin), decrease termination efficiency and are currently being evaluated for ...
Célia Floquet, Isabelle Hatin, Jean-Pierre Rousset, Laure Bidou   +3 more
doaj   +1 more source

Condensation properties of stress granules and processing bodies are compromised in myotonic dystrophy type 1

Disease Models & Mechanisms, 2022
RNA regulation in mammalian cells requires complex physical compartmentalisation, using structures thought to be formed by liquid-liquid phase separation. Disruption of these structures is implicated in numerous degenerative diseases.
Selma Gulyurtlu, Monika S. Magon, Patrick Guest, Panagiotis P. Papavasiliou, Kim D. Morrison, Alan R. Prescott, Judith E. Sleeman   +6 more
doaj   +1 more source

The Importance of the Fifth Nucleotide in DNA: Uracil

, 2023
Uracil is a ribonucleotide found in both DNA and RNA, with the main difference between the two being the presence of thymine in DNA and uracil in RNA. Although thymine and uracil are similar in function and can form the same base pairs with adenine, the presence of uracil in DNA can affect DNA stability and modulate cell-specific functions.
Roberts, Jamie Z., LaBonte , Melissa J.
openaire   +3 more sources