Results 61 to 70 of about 22,593 (200)

Bacteriophage-related epigenetic natural and non-natural pyrimidine nucleotides and their influence on transcription with T7 RNA polymerase

Communications Chemistry
DNA modifications on pyrimidine nucleobases play diverse roles in biology such as protection of bacteriophage DNA from enzymatic cleavage, however, their role in the regulation of transcription is underexplored.
Filip Gracias, Radek Pohl, Veronika Sýkorová, Michal Hocek   +3 more
doaj   +1 more source

Multiple Residues in the Second Extracellular Loop Are Critical for M3 Muscarinic Acetylcholine Receptor Activation [PDF]

, 2007
Recent studies suggest that the second extracellular loop (o2 loop) of bovine rhodopsin and other class I G protein-coupled receptors (GPCRs) targeted by biogenic amine ligands folds deeply into the transmembrane receptor core where the binding of cis ...
Kim, Soo-Kyung, Li, Bo, Scarselli, Marco, Wess, Jürgen   +3 more
core   +1 more source

Regioselective and Anisotropic Multi‐Patching of Small Microparticles via a Polymer Brush‐Assisted Microcontact Printing (µCP)

Advanced Functional Materials, EarlyView.
A method to fabricate anisotropic SiO2 patchy microspheres is presented using a modified microcontact printing technique. This involves a PDMS elastomer stamp with grooved surface topography, creating a confined environment for the microspheres.
Martin Reifarth, Marcel Sperling, Richard Grobe, Pinar Akarsu, Florian Schmitt, Maurice Schmette, Steffen Tank, Katja M. Arndt, Salvatore Chiantia, Matthias Hartlieb, Alexander Böker   +10 more
wiley   +1 more source

Interactions of slow electrons with biomolecules [PDF]

, 2007
We report on results of computational studies of the interaction of slow electrons with the purine and pyrimidine bases of DNA, as well as with their associated nucleosides and nucleotides.
McKoy, Vincent, Winstead, Carl
core   +1 more source

The Hyperthermophilic Euryarchaeon Archaeoglobus fulgidus Repairs Uracil by Single-Nucleotide Replacement [PDF]

Journal of Bacteriology, 2010
ABSTRACT Hydrolytic deamination of cytosine to uracil in cellular DNA is a major source of C-to-T transition mutations if uracil is not repaired by the DNA base excision repair (BER) pathway. Since deamination increases rapidly with temperature, hyperthermophiles, in particular, are expected to succumb to such damage.
Marivi N. Moen, Marivi N. Moen, Ingeborg Knævelsrud, Ingeborg Knævelsrud, Svein Bjelland, Arne Klungland, Gyri Teien Haugland, Nils-Kåre Birkeland, Kristin Grøsvik, Ingar Leiros   +9 more
openaire   +3 more sources

Microbiome Single Cell Atlases Generated with a Commercial Instrument

Advanced Science, EarlyView.
Single cell sequencing is valuable for dissecting complex systems and uncovering unique features in individual cell types. However, tools for single cell microbial analysis are limited. Easily Accessible Single microbe sequencing (EASi‐seq), which adapts a commercially available single cell platform for efficient sequencing of single microbial genomes ...
Xiangpeng Li, Linfeng Xu, Benjamin Demaree, Cecilia Noecker, Jordan E. Bisanz, Daniel W. Weisgerber, Cyrus Modavi, Peter J. Turnbaugh, Adam R. Abate   +8 more
wiley   +1 more source

Control of Nucleotide Metabolism and Ribosomal Ribonucleic Acid Synthesis During Nitrogen Starvation of \u3cem\u3eEscherichia coli\u3c/em\u3e [PDF]

, 1972
Ribosomal ribonucleic acid (RNA) synthesis and ribonucleoside triphosphate metabolism were studied in cultures of Escherichia coli subjected to starvation for inorganic nitrogen.
Irr, Joseph D.
core   +1 more source

Uracil in duplex DNA is a substrate for the nucleotide incision repair pathway in human cells [PDF]

Proceedings of the National Academy of Sciences, 2013
Significance Hydrolytic deamination of cytosine to uracil generates a highly mutagenic DNA base lesion and is considered one of the major sources of spontaneous mutation in living organisms. We report that the major human apurinic/apyrimidinic (AP) endonuclease, APE1, is a deoxyuridine endonuclease and can remove uracil residues in the DNA ...
Alexander A. Ishchenko, Doria Alili, Paulina Prorok, Didier Gasparutto, Murat Saparbaev, Barbara Tudek, Barbara Tudek, Christine Saint-Pierre, Dmitry O. Zharkov, Dmitry O. Zharkov   +9 more
openaire   +4 more sources

Engineered Cas12j‐8 is a Versatile Platform for Multiplexed Genome Modulation in Mammalian Cells

Advanced Science, EarlyView.
Engineered through structure‐guided protein engineering, enCas12j‐8 significantly enhances the editing efficiency of compact Cas12j‐8 while maintaining high specificity. It enables efficient multiplexed genome editing and base editing using a single crRNA array, demonstrating broad applicability and therapeutic potential in genome engineering ...
Ru Meng, Jiayao Li, Wuke Wang, Dong Liang, Zhanwei Li, Cong Mao, Qingyang Li, Yu Zhang, Hao Chen, Jin Tang, Ping Hu, Qi Niu, Xingxu Huang, Bin Shen, Jun Zhang   +14 more
wiley   +1 more source

Plasmonic Molecular Entrapment for Label‐Free Methylated DNA Detection and Machine‐Learning Assisted Quantification

Advanced Science, EarlyView.
Plasmonic molecular entrapment (PME) is label‐free detection and quantification method for DNA methylation. This technique provides direct hotspot site generation around target analytes, overcoming mismatch geometrical properties. The PME demonstrates superior SERS performance and quantification capability, offering promising potential for epigenetic ...
Muhammad Shalahuddin Al Ja'farawy, Vo Thi Nhat Linh, Chaewon Mun, Jun‐Yeong Yang, Jun Young Kim, Rowoon Park, Sung‐Gyu Park, Dong‐Ho Kim, Min‐Young Lee, Ho Sang Jung   +9 more
wiley   +1 more source